MOs were isolated from PBMCs based on differential plastic adherence [27 (link)]. Briefly, PBMCs were cultivated in RPMI-1640 supplemented with 10% FCS and 50 μg/mL gentamicin, and seeded at 2 x 106 cell/mL into 24-well plates. Cells were allowed to adhere for 2 h at 37°C before removal of non-adherent cells were and treatment of adherent MOs with MET. Cells were counted microscopically (Zeiss, Germany) using trypan blue staining, and the purity of monocytes was evaluated by fluorescent staining with PhycoErytherin (PE)-anti-human CD14 antibody (BD Biosciences, San Diego, CA, USA) using a Floid Cell Imaging Station (Thermo Fisher Scientific, MA USA) [28 (link),29 (link)] and routinely exceeded over 90% purity (S1 Fig ).
Phycoerytherin pe anti human cd14 antibody
Manufactured by BD
Sourced in United States
PhycoErytherin (PE)-anti-human CD14 antibody is a fluorescently labeled antibody that binds to the CD14 cell surface receptor on human cells. It can be used for the detection and identification of CD14-expressing cells in various applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using phycoerytherin pe anti human cd14 antibody
1
Isolation and Characterization of Monocytes
2
Isolation of Monocytes from PBMCs
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MOs were isolated from PBMCs based on differential plastic adherence (22) (link). Briefly, PBMCs were cultivated in RPMI-1640 supplemented with 10% FCS and 50 μg/mL gentamicin, and seeded at 2 x 10 6 cell/mL into 24-well plates. Cells were allowed to adhere for 2 h at 37 °C before removal of non-adherent cells were and treatment of adherent MOs with MET. Cells were counted microscopically (Zeiss, Germany) using trypan blue staining, and the purity of monocytes was evaluated by fluorescent staining with PhycoErytherin (PE)-anti-human CD14 antibody (BD Biosciences, San Diego, CA, USA) using a Floid Cell Imaging Station (Thermo Fisher Scientific, MA USA) (23, (link)24) (link) and routinely exceeded over 90% purity.
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