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Krebs buffer

Manufactured by Cayman Chemical
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Krebs buffer is a balanced salt solution commonly used in biological research. It is designed to maintain physiological pH and ionic concentrations to support the function of cells and tissues in vitro.

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Lab products found in correlation

Lucigenin, by Cayman Chemical (2 mentions) Fibrin coated 6 well plates, by Greiner (2 mentions) Nadph substrate, by Merck Group (2 mentions)

2 protocols using krebs buffer

1

Assaying NADPH Oxidase Activity in BMDMs

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BMDMs were plated on 25 μg/ml fibrin-coated 6-well plates (Greiner Bio-One). 20 μg/ml 5B8 or IgG2b were added to the fibrin plates 2 h before plating cells. NADPH oxidase activity was assayed by the lucigenin-enhanced chemiluminescence method61 (link). Cells were collected by a cell scraper and homogenized in ice-cold Krebs buffer pH 7.4 (119 mM NaCl, 2.5 mM KCl, 1mM NaH2PO4, 1.3 mM MgCl2, 2.5 mM CaCl2, 11 mM glucose, and 20 mM HEPES, pH 7.4). The cell homogenate was centrifuged at 1000g, and the pellet was resuspended with luminescence buffer (Krebs buffer containing 10 μM lucigenin, Cayman Chemicals), before adding 100 μM NADPH substrate (Sigma-Aldrich). Luminescence was detected by an EnSpire microplate reader.
Ryu J.K., Rafalski V.A., Meyer-Franke A., Adams R.A., Poda S.B., Coronado P.E., Pedersen L.Ø., Menon V., Baeten K.M., Sikorski S.L., Bedard C., Hanspers K., Bardehle S., Mendiola A.S., Davalos D., Machado M.R., Chan J.P., Plastira I., Petersen M.A., Pfaff S.J., Ang K.K., Hallenbeck K.K., Syme C., Hakozaki H., Ellisman M.H., Swanson R.A., Zamvil S.S., Arkin M.R., Zorn S.H., Pico A.R., Mucke L., Freedman S.B., Stavenhagen J.B., Nelson R.B, & Akassoglou K. (2018). Fibrin-targeting immunotherapy protects against neuroinflammation and neurodegeneration. Nature immunology, 19(11), 1212-1223.
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2

Assaying NADPH Oxidase Activity in BMDMs

Check if the same lab product or an alternative is used in the 5 most similar protocols
BMDMs were plated on 25 μg/ml fibrin-coated 6-well plates (Greiner Bio-One). 20 μg/ml 5B8 or IgG2b were added to the fibrin plates 2 h before plating cells. NADPH oxidase activity was assayed by the lucigenin-enhanced chemiluminescence method61 (link). Cells were collected by a cell scraper and homogenized in ice-cold Krebs buffer pH 7.4 (119 mM NaCl, 2.5 mM KCl, 1mM NaH2PO4, 1.3 mM MgCl2, 2.5 mM CaCl2, 11 mM glucose, and 20 mM HEPES, pH 7.4). The cell homogenate was centrifuged at 1000g, and the pellet was resuspended with luminescence buffer (Krebs buffer containing 10 μM lucigenin, Cayman Chemicals), before adding 100 μM NADPH substrate (Sigma-Aldrich). Luminescence was detected by an EnSpire microplate reader.
Ryu J.K., Rafalski V.A., Meyer-Franke A., Adams R.A., Poda S.B., Coronado P.E., Pedersen L.Ø., Menon V., Baeten K.M., Sikorski S.L., Bedard C., Hanspers K., Bardehle S., Mendiola A.S., Davalos D., Machado M.R., Chan J.P., Plastira I., Petersen M.A., Pfaff S.J., Ang K.K., Hallenbeck K.K., Syme C., Hakozaki H., Ellisman M.H., Swanson R.A., Zamvil S.S., Arkin M.R., Zorn S.H., Pico A.R., Mucke L., Freedman S.B., Stavenhagen J.B., Nelson R.B, & Akassoglou K. (2018). Fibrin-targeting immunotherapy protects against neuroinflammation and neurodegeneration. Nature immunology, 19(11), 1212-1223.
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