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Anti ngf

Manufactured by Alomone
Sourced in Israel

Anti-NGF is a laboratory reagent used for research purposes. It is a monoclonal antibody that binds to and neutralizes nerve growth factor (NGF), a protein involved in the growth and survival of certain nerve cells.

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3 protocols using anti ngf

1

Purified Ly6G+ Cell Injection for ONC

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Purified Ly6G+ cells were resuspended in sterile PBS at a concentration of 1 × 105 cells/μl and loaded into a Hamilton syringe with a 30 G needle. In some cases, anti-NGF (Alomone) and/ or anti-IGF1 (Abcam) polyclonal antibodies, or polyclonal control antibodies (Sigma), were included in the cell suspension, at a final concentration of 1 μg/μl. 3 μl of cell suspension was injected into the posterior chamber of each eye of anesthesized mice on days 0 and 3 post ONC injury.
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2

Immunoblotting of NGF and p75NTR

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The following antibodies were used for immunoblotting: rabbit polyclonal anti-NGF and anti-proNGF (Alomone Labs, Israel) and rabbit polyclonal p75NTR (1 : 5000), a kind gift from Dr. Bruce Carter, Vanderbilt University, Nashville, TN. Secondary antibodies were horseradish peroxidase-conjugated goat anti-rabbit (Calbiochem, La Jolla, CA).
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3

Purified Ly6G+ Cell Injection for ONC

Check if the same lab product or an alternative is used in the 5 most similar protocols
Purified Ly6G+ cells were resuspended in sterile PBS at a concentration of 1 × 105 cells/μl and loaded into a Hamilton syringe with a 30 G needle. In some cases, anti-NGF (Alomone) and/ or anti-IGF1 (Abcam) polyclonal antibodies, or polyclonal control antibodies (Sigma), were included in the cell suspension, at a final concentration of 1 μg/μl. 3 μl of cell suspension was injected into the posterior chamber of each eye of anesthesized mice on days 0 and 3 post ONC injury.
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