MS1 cells were lysed with lysis buffer containing 20 mM Tris·HCl, 2 mM EDTA, 0.5% NP-40, 1 mM NaF and 1 mM Na3VO4, and protease inhibitor cocktail. The samples were heated to 95° for 5 min with loading buffer containing 0.5% 2-mercaptoethanol. Equal amount of samples was loaded and resolved on a 10% SDS-PAGE gel. Proteins were then transferred to PVDF membrane (Millipore, 0.45 μm). The membranes were first incubated with anti-phospho-Src (Tyr416) antibody (CST) or anti-phospho-Src (Tyr529) antibody (Abcam) followed by Goat anti-Rabbit HRP (CWBIO, China). The blot was developed by chemiluminescent HRP substrate (ECL, Millipore). The blot was then stripped and reblotted with anti-β-actin antibody (Zsbio, China) subsequently. The images were captured on a Tanon 5200 chemi-image system (Tanon, China). Gel images were quantified with Lane 1D analysis software (SageCreation, China).
Anti phospho src tyr529 antibody
Manufactured by Abcam
Sourced in China
Anti-phospho-Src (Tyr529) antibody is a primary antibody that recognizes the phosphorylated form of the Src protein at tyrosine residue 529. The antibody can be used to detect and analyze the activation state of Src, a non-receptor tyrosine kinase involved in various cellular processes.
Automatically generated - may contain errors
2 protocols using anti phospho src tyr529 antibody
1
Phospho-Src Immunoblotting Protocol
2
Quantitative Western Blot Analysis
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MS1 cells were lysed with lysis buffer containing 20mM Tris•HCl, 2mM EDTA, 0.5% NP-40, 1mM NaF and 1mM Na3VO4 and protease inhibitor cocktail. The samples were heated to 95℃ for 5 minutes with loading buffer containing 0.5% 2-Mercaptoethanol.
Equal amount of samples was loaded and resolved on a 10% SDS-PAGE gel. Proteins were then transferred to PVDF membrane (Millipore, 0.45μm). The membranes were first incubated with anti-phospho-Src (Tyr416) antibody (CST) or anti-phospho-Src (Tyr529) antibody (Abcam) followed by Goat anti-Rabbit HRP (CWBIO, China). The blot was developed by chemiluminescent HRP substrate (ECL, Millipore). The blot was then stripped and reblotted with anti-β-actin antibody (Zsbio, China) subsequently. The images were captured on a Tanon 5200 chemi-image system (Tanon, China). Gel images were quantified with Lane 1D analysis software (SageCreation, China).
Equal amount of samples was loaded and resolved on a 10% SDS-PAGE gel. Proteins were then transferred to PVDF membrane (Millipore, 0.45μm). The membranes were first incubated with anti-phospho-Src (Tyr416) antibody (CST) or anti-phospho-Src (Tyr529) antibody (Abcam) followed by Goat anti-Rabbit HRP (CWBIO, China). The blot was developed by chemiluminescent HRP substrate (ECL, Millipore). The blot was then stripped and reblotted with anti-β-actin antibody (Zsbio, China) subsequently. The images were captured on a Tanon 5200 chemi-image system (Tanon, China). Gel images were quantified with Lane 1D analysis software (SageCreation, China).
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