Standard stock solution (gallic acid, gentisic acid, [1]-catechin, chlorogenic acid, caffeic acid, [2]-epicatechin, p-coumaric acid, sinapic acid, benzoic acid, p-anisic acid, myricetin, 3,4,5-trimethoxycinnamic acid and quercetin) was prepared in methanol at concentrations of 0.2–1 mg/mL, and filtered through a membrane filter (0.45 mm). Phenolic compounds in blueberry extracts were analyzed using an reversed-phase HPLC with a Waters 600 pump controller equipped with an Eclipse XDB-C18 reversed-phase column (25 cm × 4.6 mm ID × 5 μm) (Supelco; Sigma-Aldrich Co.). Compounds were eluted with a mobile phase solvent A (ddH2O, pH 2.5 with trifluoroacetic acid) and solvent B (methanol). Gradient elution step started with 100% solvent A at 0 min until 50% solvent A and 50% solvent B for 40 min. Flow rate was 1.0 mL/min. The detection wavelength was set at four different UV wavelengths, 280, 320, and 370 nm because the compound mixtures have different UV spectra.
Waters 600 pump controller
Manufactured by Waters Corporation
Sourced in United States
The Waters 600 pump controller is a laboratory equipment device designed to provide precise control and management of liquid chromatography pumps. It is a core component used in analytical chemistry and biochemistry workflows to facilitate the accurate delivery of solvents and mobile phases during chromatographic separations.
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2 protocols using waters 600 pump controller
1
HPLC Analysis of Phenolic Compounds in Blueberry Extracts
2
Molecular Weight Analysis of HA-Nimesulide Aqueous Solution
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The molecular weight distribution of HA and HAH–nimesulide aqueous solution was assessed by the GPC system consisting of a Waters 600 pump controller (Waters, Milford, MA, USA), an injection valve with a 200 µL sample loop, ultrahydrogel 2,000 and 250 columns 7.8×300 mm (Waters), a 2,414 refractive index detector (Waters) and a photo diode array (set to 254 nm, the ~λmax of nimesulide; Waters), using the mobile phase 0.02 M NH4H2PO4(aq) and 0.3 M NaNO3(aq) at 40°C at a flow rate of 0.7 mL/min.
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