Nucleic acids extracted from purified IBs were treated with RNAse A (Thermo Scientific™, 10 mg/ml), RNase T1 (Thermo Scientific™, 1000 U/ml), DNAse I, restriction endonucleases XhoI and HindIII (New England BioLabs) and Proteinase K (Roche, 10 μg/ml) following the manufacturers’ protocols.
Restriction endonucleases xhoi and hindiii
Manufactured by New England Biolabs
Restriction endonucleases XhoI and HindIII are enzymes that recognize and cleave specific DNA sequences. XhoI recognizes and cleaves the palindromic DNA sequence 5'-CTCGAG-3', while HindIII recognizes and cleaves the palindromic DNA sequence 5'-AAGCTT-3'.
Automatically generated - may contain errors
2 protocols using restriction endonucleases xhoi and hindiii
1
Purified Inclusion Body Nucleic Acid Analysis
2
Nucleic Acids Extraction and Purification
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Nucleic acids extracted from purified IBs were treated with RNAse A (Thermo Scientific™, 10 mg/mL), RNase T1 (Thermo Scientific™, 1000 U/ml), DNAse I, restriction endonucleases XhoI and HindIII (New England BioLabs) and Proteinase K (Roche, 10 μg/ml) following the manufacturers' protocols.
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