Sprague Dawley (SD) rats were obtained from Sankyo Labo Service Corporation (Tokyo, Japan) and housed in temperature-controlled cages (25 °C ± 5 °C) with a 12 h:12 h light-dark cycle. Rats were fed a balanced commercial rat chow (CE-2, Clea Japan, Inc., Tokyo, Japan) and allowed water ad libitum. The Keio University Animal Research Committee approved all animal procedures performed in this study. Rats were euthanized with an overdose of 5% isoflurane inhalation (Wako Pure Chemical Industries Ltd., Osaka, Japan). All of the animals in this work were treated according to the Association for Research in Vision and Ophthalmology (ARVO) Statement for the Use of Animals in Ophthalmic and Vision Research.
Isoflurane inhalation
Manufactured by Fujifilm
Sourced in Japan
Isoflurane inhalation is a medical-grade anesthetic agent used in laboratory settings. It is a volatile liquid that is vaporized and administered through inhalation to induce and maintain general anesthesia in animals. The key function of Isoflurane inhalation is to provide a controlled and reliable method for administering anesthesia during surgical or other procedures.
Automatically generated - may contain errors
3 protocols using isoflurane inhalation
1
Sprague Dawley Rat Housing Protocol
2
Ovalbumin-based Allergic Asthma Model
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The following reagents were purchased: phosphate buffered saline (PBS) solution (Nissui Pharmaceutical Co., Tokyo, Japan); ovalbumin (OA), aluminum hydroxide gel, isoflurane inhalation, May-Grunwald staining solution, and Giemsa’s staining solution (Wako Pure Chemical Industries, Osaka, Japan).
3
Mandibular Condyle Histology in Aging Mice
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The 10-and 50-week-old mice were euthanized by isoflurane inhalation (FUJIFILM Wako Pure Chemical Corporation), and the mandibular condyles were resected and fixed in 4% paraformaldehyde in 0.1-m phosphate buffer (pH 7.4) at 4°C overnight. The fixed specimens obtained from 10-and 50-week-old mice were decalcified in 10% ethylenediamine tetra-acetic acid in 0.01-m phosphate buffer at 4°C for 1.5 and 2 months, respectively. After dehydrating through a graded series of ethanol solutions, the specimens were embedded in paraffin and sectioned coronally to a thickness of 5 μm. Serial sections, including the central part of the mandibular condyle, were selected, some of which were stained with hematoxylin and eosin.
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