BCP concentrations in serum and organ samples were analyzed using a GC–MS (Agilent 7890B-5977B MSD; Agilent Technologies, Santa Clara, CA, USA), a Thermal Desorption Unit (TDU2), Programmable Temperature Vaporization inlet (CIS4), and Multipurpose Sampler (MPS) with Dynamic Head Space (DHS) option (Gerstel GmbH & Co.KG, Mülheim an der Ruhr, Germany)39 (link). An MSD ChemStation vs. F. 01.03. 2357 (Agilent) and Mass Hunter v.B.07.05.2479 (Agilent) were used for data analysis.
Tenax TA cartridges were released from Tenax TA traps using a thermal desorption cold-trap setup (thermal desorption spectrometer (TDS); Markes International, Ltd., Llantrisant, RCT, UK). An InertCap WAX column (length: 60 m, df: 0.25 μm, I.D.: 0.25 mm, GL Sciences, Inc., Tokyo, Japan) was used for component separation. The oven temperature was programmed as follows: initial temperature, 40 °C; ramp rate, 3 °C/min (40 to 145 °C) and 10 °C/min (145 to 240 °C); and final temperature, 240 °C for 5 min. The He inlet pressure was controlled using an electronic pressure control system to achieve a constant column flow of 1 mL/min. MS analysis was performed in the ionization mode at a voltage of 70 eV.
Tenax TA cartridges were released from Tenax TA traps using a thermal desorption cold-trap setup (thermal desorption spectrometer (TDS); Markes International, Ltd., Llantrisant, RCT, UK). An InertCap WAX column (length: 60 m, df: 0.25 μm, I.D.: 0.25 mm, GL Sciences, Inc., Tokyo, Japan) was used for component separation. The oven temperature was programmed as follows: initial temperature, 40 °C; ramp rate, 3 °C/min (40 to 145 °C) and 10 °C/min (145 to 240 °C); and final temperature, 240 °C for 5 min. The He inlet pressure was controlled using an electronic pressure control system to achieve a constant column flow of 1 mL/min. MS analysis was performed in the ionization mode at a voltage of 70 eV.