Mouse monoclonal anti mmp 2

Tenocytes were lysates, and 15 µg of each sample was separated on a 4–20% SDS-PAGE Gel by electrophoresis (ExpressPlus™ 10x8, GenScript Biotech Corporation, Nanjing, China). After running, the samples were transferred to nylon membranes, as already described [8 (link)]. The membranes were incubated in the presence of mouse monoclonal anti-β-actin (1:10,000) (Sigma-Aldrich, St. Louis, MO, USA), mouse monoclonal anti-collagen 1A1 (1:200), collagen 3A1 (1:100), mouse monoclonal anti-iNOS (1:200), mouse monoclonal anti-MMP2 (1:200) (all from Santa Cruz Biotechnology, Santa Cruz, CA, USA), and rabbit monoclonal anti-MMP14 (1:1000) (purchased from Abcam). After an overnight incubation at 4 °C with primary antibodies under gentle shaking, membranes were then probed with specific IgG horseradish peroxidase (HRP)-conjugated secondary antibodies and bands were identified by chemiluminescence as previously described [8 (link)]. At least three independent experiments were performed for each protein. Results are expressed as mean values ± S.D. of normalized densitometric values on β-actin.