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Nefa kit hr series nefa hr 2

Manufactured by Fujifilm
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Sourced in Japan

The NEFA-Kit (HR Series NEFA-HR (2)) is a laboratory equipment product manufactured by Fujifilm. It is designed for the quantitative determination of non-esterified fatty acids (NEFA) in biological samples.

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Lab products found in correlation

Microvette tube, by Sarstedt (1 mentions) Precision xtra, by Abbott (1 mentions) Accu chek, by Roche (1 mentions)

Close spelling variants of this product

NEFA-HR(2), (75 citations) NEFA kit (72 citations) NEFA-HR(2) kit (50 citations) NEFA-HR (40 citations) NEFA-HR kit (38 citations) HR Series NEFA-HR(2), (36 citations) HR Series NEFA-HR (24 citations) HR Series NEFA-HR(2) kit (16 citations) HR Series NEFA kit (6 citations) HR series NEFA-HR kit (4 citations)

2 protocols using nefa kit hr series nefa hr 2

1

Blood Analysis of Fasted and Fed Mice

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Blood analysis was carried out in the morning of ad-libitum fed or 24 h fasted animals. Blood glucose and ketone bodies were measured from tail blood with a glucose meter (Accu-Chek, Roche) or a ketone body meter (Precision Xtra, Abbott Laboratories), respectively. Plasma was obtained from whole blood, which was collected in microvette tubes (Sarstedt) and centrifuged at 2000 g for 10 min. Non-esterified fatty acids (NEFA) were measured in plasma using a NEFA-Kit (HR Series NEFA-HR (2), Wako Diagnostics) according to the manufacturer's instructions.
Schmid S., Heim-Kupr B., Pérez-Schindler J., Mansingh S., Beer M., Mittal N., Ehrenfeuchter N, & Handschin C. (2022). PGC-1β modulates catabolism and fiber atrophy in the fasting-response of specific skeletal muscle beds. Molecular Metabolism, 66, 101643.
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2

Measuring Metabolic Biomarkers in Mice

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Plasma NEFA levels were analysed using the NEFA kit HR Series NEFA-HR(2) from WAKO Chemicals, Japan) according to manufactures instructions. Liver TG levels were measured from liver Folch extracts (Folch, Lees, & Sloane Stanley, 1957) using the Triglycerides FS 10 kit (Diasys, Germany, 15760991002) . Samples where dissolved in 600 µl 1% Triton X-100. Plasma insulin levels were determined using the mouse insulin ELISA kit (Chrystal Chem, USA, 90080).
Wieser B.I., Sancha P.P.d.l., Schauer S., Reicher H., Sattler W., Breinbauer R., Schweiger M., Espenshade P.J., Zechner R., Höefler G., & Vesely P. (2020). Adipose Triglyceride Lipase is needed for homeostatic control of Sterol Element-Binding Protein-1c driven hepatic lipogenesis.
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