Antibodies were from the following sources: anti-ERK1/2 P-thr 202 and P-tyr 204 #9101, anti-ERK1/2 #9102, anti-MEK1/2 P-ser 217 and P-ser 221 #9121, anti-MEK1/2 #9122 (Cell Signaling, Danvers, MA, USA), and anti MKP-1/DUSP1 (E-6)(Santa Cruz Biotechnology, Dallas, TX, USA). Antibodies to GC-A were generated and used as described (46 (link)). ELISA kits for cGMP and aldosterone were from ENZO Lifesciences, Inc. (Farmingdale, New York, USA). The testosterone ELISA was from Cayman Chemical (Ann Arbor, MI56t). ELISAs for mouse ANP and mouse BNP were from RayBiotech (Peachtree Corners, GA, USA). fsANP was from Anaspec (Fremont, CA, USA) as previously described (47 (link)). The mouse creatinine assay kit was from Crystal Chem (Elk Grove Village, IL, USA).
Testosterone elisa
Manufactured by Cayman Chemical
The Testosterone ELISA kit is a quantitative test used to measure the concentration of testosterone in biological samples. It is a competitive enzyme-linked immunosorbent assay (ELISA) that utilizes a testosterone-specific antibody to detect and quantify the target analyte.
Automatically generated - may contain errors
2 protocols using testosterone elisa
1
Biochemical Signaling Pathway Assays
2
Androstenedione-Induced Testosterone Production
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were seeded in type IV collagen-coated 6-well tissue culture plates and allowed to reach 90% confluence. Cells were treated in keratinocyte growth medium without supplements as indicated in each experiment.
Treatment medium was aspirated, and cells were washed three times in phosphate buffered saline (PBS). Cells were then incubated in keratinocyte growth media containing 1 nmol/L androstenedione for various times as indicated (final volume ¼ 1.2 ml/well). Supernatant and protein were extracted and kept at e80 C until used for analysis. Testosterone formation was assessed by testosterone ELISA (Cayman Chemical, Ann Harbor, MI) according to manufacturer's instructions. All experiments were performed in duplicate, and each duplicate was assessed three times on the ELISA plate. Results were averaged and normalized to total protein as determined by the BCA Protein Assay kit (Thermo Fisher Scientific, Waltham, MA).
Treatment medium was aspirated, and cells were washed three times in phosphate buffered saline (PBS). Cells were then incubated in keratinocyte growth media containing 1 nmol/L androstenedione for various times as indicated (final volume ¼ 1.2 ml/well). Supernatant and protein were extracted and kept at e80 C until used for analysis. Testosterone formation was assessed by testosterone ELISA (Cayman Chemical, Ann Harbor, MI) according to manufacturer's instructions. All experiments were performed in duplicate, and each duplicate was assessed three times on the ELISA plate. Results were averaged and normalized to total protein as determined by the BCA Protein Assay kit (Thermo Fisher Scientific, Waltham, MA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!