Cells were grown on sterile glass slides, xed with 4% paraformaldehyde for 30 min, blocked with 1% BSA for 30 min, incubated with primary antibodies against CDH1 (E-cadherin, AF0131, 1:500; A nity Biosciences LTD.), CDH2 (Ncadherin AF4039, 1:200; A nity Biosciences LTD.), and VIM (Vimentin AF7013, 1:250; A nity Biosciences LTD.) at 4°C overnight, followed by incubation with Alexa Fluor® 594 conjugated A nipure Goat Anti-Rabbit IgG (H + L) secondary antibodies (Jackson ImmunoResearch Laboratories, PA) for 1 hour. Nuclei were counterstained with DAPI. Images were captured with Olympus IX81 inverted uorescence microscope (Olympus, Beijing, China).
Alexa fluor 594 conjugated a nipure goat anti rabbit igg h l secondary antibodies
Manufactured by Jackson ImmunoResearch
Alexa Fluor® 594 conjugated AffiniPure Goat Anti-Rabbit IgG (H + L) secondary antibodies are designed for the detection of rabbit primary antibodies in immunoassays. The antibodies are conjugated with the fluorescent dye Alexa Fluor® 594, which has an excitation wavelength of 590 nm and an emission wavelength of 617 nm.
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Lab products found in correlation
2 protocols using alexa fluor 594 conjugated a nipure goat anti rabbit igg h l secondary antibodies
1
Immunofluorescence Staining of Cell-Cell Adhesion
2
Immunofluorescence Staining of Cell-Cell Adhesion
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Cells were grown on sterile glass slides, xed with 4% paraformaldehyde for 30 min, blocked with 1% BSA for 30 min, incubated with primary antibodies against CDH1 (E-cadherin, AF0131, 1:500; A nity Biosciences LTD.), CDH2 (Ncadherin AF4039, 1:200; A nity Biosciences LTD.), and VIM (Vimentin AF7013, 1:250; A nity Biosciences LTD.) at 4°C overnight, followed by incubation with Alexa Fluor® 594 conjugated A nipure Goat Anti-Rabbit IgG (H + L) secondary antibodies (Jackson ImmunoResearch Laboratories, PA) for 1 hour. Nuclei were counterstained with DAPI. Images were captured with Olympus IX81 inverted uorescence microscope (Olympus, Beijing, China).
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