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Cholesterol ovine wool

Manufactured by Avanti Polar Lipids
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Sourced in United States

Cholesterol (ovine wool) is a pure chemical compound derived from ovine wool. It is a naturally occurring sterol that serves as a structural component of cell membranes. This product is suitable for use in various laboratory applications that require a consistent, high-purity source of cholesterol.

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Lab products found in correlation

Glutamax, by Thermo Fisher Scientific (4 mentions) Mouse il 34, by R&D Systems (2 mentions) Tgf β, by Thermo Fisher Scientific (2 mentions) Chloroform, by Carl Roth (1 mentions) Sodium hydroxide (naoh), by Thermo Fisher Scientific (1 mentions) Milli q advantage a10, by Merck Group (1 mentions) Topfluor cholesterol, by Avanti Polar Lipids (1 mentions) Porcine brain l α phosphatidylcholine, by Avanti Polar Lipids (1 mentions) Sodium chloride (nacl), by Merck Group (1 mentions) Avanti mini extruder, by Avanti Polar Lipids (1 mentions) L α phosphatidylcholine, by Avanti Polar Lipids (1 mentions) 1.2 dipalmitoyl sn glycero 3 phospho 1 rac glycerol sodium salt dppg, by Avanti Polar Lipids (1 mentions) Skf81297 hydrobromide, by Bio-Techne (1 mentions) Calcein sodium salt, by Thermo Fisher Scientific (1 mentions) Dspe peg2000, by Avanti Polar Lipids (1 mentions) Speedvac, by Labconco (1 mentions)

Close spelling variants of this product

Cholesterol from ovine wool Ovine cholesterol Cholesterol

6 protocols using cholesterol ovine wool

1

Lipid and Protein Preparation for Biophysical Studies

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The lipids porcine brain L-α-phosphatidylcholine (PC), porcine brain L-α-phosphatidylserine (PS), porcine brain L-α-phosphatidylethanolamine (PE), porcine brain sphingomyelin (SM), bovine liver L-α-phosphatidylinositol (PI), and cholesterol (ovine wool) were purchased from Avanti Polar Lipids (Alabaster, USA). Bovine myelin basic protein 18.5 kDa was purchased from Merck KGaA (Darmstadt, Germany). Buffer solution of N-(2-hydroxyethyl)piperazine-N’-ethanesulfonic acid (HEPES) and sodium chloride (both from Merck KGaA) was prepared with ultrapure water from a Milli-Q Advantage A10 (Millipore S.A.S., Molsheim Cédex, France) with a conductivity lower than 0.055 µS/cm, and it was adjusted with sodium hydroxide (Fisher Scientific, Leicestershire, UK) to pH 7.4. The chloroform used had HPLC grade and was purchased from Carl Roth GmbH & Co. KG (Karlsruhe, Germany). The fluorescent dye 1,2-Dihexadecanoyl-sn-glycero-3-phosphoethanol-amine-N-(lissamine rhodamine B sulfonyl) (Rh−DHPE) was obtained from Life Technologies GmbH (Darmstadt, Germany) and TopFluor® Cholesterol was obtained from Avanti Polar Lipids (Alabaster, USA). All chemicals were used as received without further purification.
Träger J., Widder K., Kerth A., Harauz G, & Hinderberger D. (2020). Effect of Cholesterol and Myelin Basic Protein (MBP) Content on Lipid Monolayers Mimicking the Cytoplasmic Membrane of Myelin. Cells, 9(3), 529.
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2

Lipid-based Nanoparticle Formulation

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1.2-dipalmytoil-sn-glycero-3-phosphocholine (DPPC), cholesterol (ovine wool), L-α-phosphatidylcholine (Soy PC), 1.2-dipalmitoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (sodium salt) (DPPG) and Avanti Mini-Extruder were purchased from Avanti Polar Lipids, Alabama. Histamine dihydrochloride, poly (ethylene glycol) (PEG, MW 8000), dextran (from Leuconostoc spp. MW 450000-600000) were obtained from Sigma-Aldrich, Sweden. Whatman nucleopore track-etched polycarbonate membranes, pore size 5 μm and Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (10K MWCO) were purchased from Fisher Scientific, Sweden.
Lovrić J., Keighron J.D., Angerer T.B., Li X., Malmberg P., Fletcher J.S, & Ewing A.G. (2014). Analysis of liposome model systems by time-of-flight secondary ion mass spectrometry. Surface and interface analysis : SIA, 46(Suppl 1), 74-78.
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3

Lipid-Dye Preparation for Cellular Imaging

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DSPC (> 99%), DSPE- PEG2000 (> 99%) and cholesterol (ovine wool, >98%) were purchased from Avanti Polar lipids, Inc. Calcein sodium salt (644.5 g/mol, 108750-13-6) was purchased from Alfa Aesar. SKF-81297 hydrobromide (370.67 g/mol, ≥ 98%) was purchased from Tocris Bioscience. 1,1’-Dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate (DilC18(3)), ≥ 90%) was purchased from Fisher Scientific. All other chemicals were analytical grade.
Xiong H., Alberto K.A., Youn J., Taura J., Morstein J., Li X., Wang Y., Trauner D., Slesinger P.A., Nielsen S.O, & Qin Z. (2022). Optical control of neuronal activities with photoswitchable nanovesicles. Nano research, 16(1), 1033-1041.
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4

Primary Neuron Tri-Culture Protocol

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Base media (plating medium and co-culture medium) were prepared as previously described (Chapman et al., 2015) (link). Briefly, plating medium consisted of Neurobasal A culture medium supplemented with 2% B27 supplement, 1x Glutamax, 10% heat-inactivated horse serum and 1 M HEPES at pH 7.5, while the co-culture medium consisted of Neurobasal A culture medium supplemented with 2% B27 supplement and 1x Glutamax (all from ThermoFisher). The tri-culture medium consisted of supplementing the co-culture medium with 100 ng/mL mouse IL-34 (R&D Systems), 2 ng/mL TGF-β (Peprotech) and 1.5 µg/mL ovine wool cholesterol (Avanti Polar Lipids). Due to the limited shelf life of the IL-24 and TGF-β, tri-culture medium was made new each week.
Goshi N., Morgan R.K., Lein P.J., & Şeker E. (2020). A Primary Neural Cell Culture Model for Neuroinflammation.
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5

Tri-culture microglia-neuron-astrocyte protocol

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Base media (plating medium and co-culture medium) were prepared as previously described [25 (link)]. Briefly, plating medium consisted of Neurobasal A culture medium supplemented with 2% B27 supplement, 1x Glutamax, 10% heat-inactivated horse serum, and 1 M HEPES at pH 7.5, while the co-culture medium consisted of Neurobasal A culture medium supplemented with 2% B27 supplement and 1x Glutamax (all from ThermoFisher). The tri-culture medium consisted of supplementing the co-culture medium with 100 ng/mL mouse IL-34 (R&D Systems), 2 ng/mL TGF-β (Peprotech), and 1.5 μg/mL ovine wool cholesterol (Avanti Polar Lipids). Due to the limited shelf life of IL-34 and TGF-β, the tri-culture medium was made fresh each week.
Goshi N., Morgan R.K., Lein P.J, & Seker E. (2020). A primary neural cell culture model to study neuron, astrocyte, and microglia interactions in neuroinflammation. Journal of Neuroinflammation, 17, 155.
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6

Aqueous Humor Lipid Extraction Protocol

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Aqueous humor samples were subjected to extraction of lipids using suitable and minimal modification of Bligh and Dyer method [25 (link), 26 (link)]. The lower organic phase containing the extracted lipids was isolated and solvent dried with a Speed-Vac (Model 7810014; Labconco, Kansas City, MO). Samples were subsequently flushed with argon gas to prevent oxidation. Proteins recovered from the corresponding upper aqueous phase were quantified using Bradford’s method [27 (link)]. A subset of protein samples were also subjected to densitometric quantification using bovine serum albumin (BSA) as a standard (amino acid quantified) after electrophoretic separation on a PHAST (GE Healthcare Bio-Sciences AB, Sweden) gel system [28 (link)]. We also repeated protein estimations using an amino acid analyzer after overnight digestion in hydrochloric acid following previously published protocols [29 (link)]. The protein amounts determined using amino acid analyzer was utilized in normalization of lipids per amount of proteins. In order to determine and ensure extraction efficiency, ovine wool cholesterol (molecular mass 386.7; catalog no. 700000; Avanti Polar Lipids, Albaster, AL) [30 (link)] was premixed with AQH prior to extraction. All extractions and subsequent handling was made using glass vials to avoid contaminating impurities.
Edwards G., Aribindi K., Guerra Y., Lee R.K, & Bhattacharya S.K. (2014). Phospholipid Profiles of Control and Glaucomatous Human Aqueous Humor. Biochimie, 101, 232-247.
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