Glaucoma specialist (Y.H.Z.) performed all the limbus-based bleb trabeculectomy surgeries. Briefly, a 3 × 4 mm2 rectangular scleral flap was made and dissected to two-thirds of the scleral depth. MMC (0.4 mg/mL, SunRise, Shanghai, China) was applied for 2 min with balanced salt solution. Then, a piece of trabecular meshwork was excised, and peripheral iridectomy was performed. The scleral flap was sutured with two releasable sutures under mild tension, and the Tenon’s tissue was closed with 10-0 Ethilon (Ethicon, Johnson & Johnson Medical, San Angelo, TX, USA). Then, the conjunctiva was closed with a watertight suture using 8-0 Vicryl sutures (Ethicon, Johnson & Johnson Medical, San Angelo, TX, USA). If IOP was not well-controlled, the releasable sutures were removed. In addition, nd-doped yttrium aluminum garnet (ND-YAG) laser sutures lysis would be applied if the releasable sutures were eliminated.
10 0 ethilon
Manufactured by Johnson & Johnson
Sourced in United States, Japan, Belgium
10-0 Ethilon is a sterile, monofilament, non-absorbable surgical suture made of nylon. It is designed for use in ophthalmic and microsurgical procedures.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using 10 0 ethilon
1
Limbus-Based Bleb Trabeculectomy Protocol
2
Pudendal Nerve Transection and Anastomosis
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Following a circumanal skin incision, the anal canal and the sphincter muscles were circumferentially dissected from the surrounding tissue. The posterior wall of the anal segment was separated until identification of the anterior surface of the coccygeal muscle. The levator ani muscle was identified at the lateral and posterior wall, and the anterior wall was then dissected free, behind the prostate. The neurovascular bundles of the pudendal arteries, veins, and nerves run bilaterally inside the ischial tuberosity and reach the external anal sphincter muscle at the 2 o’clock and 10 o’clock positions (Fig. S1A). We then dissected the pudendal nerves to avoid damage (Fig. S1B). In the transection group (N = 3), we transected bilateral pudendal nerves and resected a 1-cm width (Fig. S1C). In the anastomosis group (N = 3), we transected bilateral pudendal nerves and then anastomosed the nerves end-to-end with epineural sutures (10-0 Ethilon; Johnson & Johnson, Tokyo, Japan) using a surgical microscope (OME-9000; Olympus Medical Systems Corp., Tokyo, Japan) (Fig. S1D). The sham operation was performed similarly but without neurotomy. All wounds were closed using skin staples (3 M Precise Vista Skin Staplers; Medema T/A Omega Medical Supplies Ltd., London, UK).
3
Median Nerve Transection and Autograft in Rats
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Ten male Lewis rats (Janvier Labs, Le Genest-Saint-Isle, France), weighing 280–350 g, were kept in groups of two or three in appropriate cages according to internal standard operating procedures. The animals had access to food and water ad libitum. After the rats were allowed to get accustomed to their new surrounding for 7 days prior to any experimental handling and after completing a 7-day training period on the CW device, they underwent bilateral surgery of the median nerve under an operation microscope (Leica M651, Leica Microsystems, Vienna, Austria). A 7-mm segment of the left and right nerve was removed microsurgically by performing a transection about 1.5 mm proximal to the position where it is crossed over by the brachial artery and vein and another transection 7 mm proximal to the first one. On the right side, the gap was bridged with the original nerve segment in reverse fashion as a homotopic nerve autograft with two sutures per coaptation site (Ethilon® 10-0, Ethicon-Johnson & Johnson, Brussels, Belgium). On the left side, the nerve defect remained unreconstructed to serve as an internal control group. To prevent spontaneous regeneration, the distal nerve stump was sutured into the short head of the biceps muscle. The post-operative observation period lasted 12 weeks (n = 10).
4
Sciatic Nerve Transection and Repair
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Sprague Dawley rats (200–250 g) were deeply anesthetized by inhalation of isoflurane, and anesthesia was maintained throughout the surgery through a mask. Analgesia (25 mg/kg Carprofen; Rimadyl®, Pfizer, UK) was administered subcutaneously. The sciatic nerve was exposed at mid-thigh level and transected to create an 8 mm gap. In both EngNT groups, the conduit was implanted by insertion of the proximal and distal nerve stumps into the 10 mm tube and coaptated to the conduit by two epineurial sutures (Ethilon 10/0; Ethicon-Johnson & Johnson, Brussels, Belgium) at each stump. Subsequently the wound was closed in layers, and animals were maintained for 4 weeks. After this period, animals were culled using CO2 asphyxiation, and their nerves were harvested under the dissecting microscope and fixed using 4% paraformaldehyde.
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