Anti tomm20 11802 1 ap

Manufactured by Proteintech

Sourced in United States

Anti-TOMM20 (11802-1-AP) is a primary antibody that recognizes the TOMM20 protein. TOMM20 is a component of the translocase of the outer mitochondrial membrane (TOM) complex, which is responsible for the import of nuclear-encoded proteins into the mitochondria.

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Lab products found in correlation

Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Anti rab5, by Cell Signaling Technology (1 mentions) Anti rabbit igg af488, by Thermo Fisher Scientific (1 mentions) Anti mouse igg af568, by Thermo Fisher Scientific (1 mentions) Anti human igg af488, by Thermo Fisher Scientific (1 mentions) Anti tigar sc 166290, by Santa Cruz Biotechnology (1 mentions) Anti parp 9532, by Cell Signaling Technology (1 mentions) Anti β actin sc 8432, by Santa Cruz Biotechnology (1 mentions) Ripa buffer, by Beyotime (1 mentions) Anti nrf2 16396 1 ap, by Proteintech (1 mentions)

2 protocols using anti tomm20 11802 1 ap

Immunofluorescence Staining of SARS-CoV-2 Proteins

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Primary and secondary antibodies were diluted at 1:500 for immunofluorescence as follows: anti-Rab5 (#3547, Cell Signaling, Danvers, MA, USA), anti-Spike S1 antibody (#100715-2, BPS, San Diego, CA, USA), anti-angiotensin converting enzyme 2 (ACE2, SC390851, Santa Cruz Biotechnology, Dallas, TX, USA), anti-TOMM20 (11802-1-AP, Proteintech, Rosemont, IL, USA), anti-rabbit IgG AF488 (#A32731, Invitrogen, Waltham, MA, USA), anti-mouse IgG AF568 (#A11004, Invitrogen), and anti-human IgG AF488 (#A11013, Invitrogen). The receptor binding domain (RBD, S1) of spike protein (BT10569, R&D Systems, Minneapolis, MN, USA) and active trimer of spike protein (10586-CV, R&D Systems) were purchased from R&D Systems.

Kim E.S., Jeon M.T., Kim K.S., Lee S., Kim S, & Kim D.G. (2021). Spike Proteins of SARS-CoV-2 Induce Pathological Changes in Molecular Delivery and Metabolic Function in the Brain Endothelial Cells. Viruses, 13(10), 2021.

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Protein Analysis of Mitochondrial Regulators

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Whole‐cell lysis was prepared with RIPA buffer (Beyotime, P0013B) and centrifuged at 12,000 g for 15 minutes. Protein concentrations were measured using Pierce® BCA protein assay kit (Thermo Scientific, 23225). Immunoblot was performed using specific primary antibodies and fluorescein‐conjugated secondary goat anti‐rabbit/mouse antibody (Gene, 926‐32211) and then detected using Odyssey fluorescence scanner.
Anti‐Parkin (4211), anti‐Ub (3936), anti‐p‐Ub (Ser65) (62802), and anti‐PARP (9532) were purchased from Cell Signaling Technology. Anti‐gankyrin (sc‐101498), anti‐BNIP3 (sc‐56167), anti‐β‐actin (sc‐8432), and anti‐TIGAR (sc‐166290) were purchased from Santa Cruz Biotechnology, Inc. Anti‐G6PD (A‐11234), anti‐PGD (A‐0563), anti‐TKT (A‐13553), anti‐FUNDC1 (A‐16318), anti‐HSP60 (A‐0564), and anti‐TALDO (A‐13551) were purchased from Abclonal. Anti‐Nrf2 (16396‐1‐AP) and anti‐TOMM20 (11802‐1‐AP) were purchased from Proteintech Inc.

Yang C., Cui X., Ding Z., Jiang T., Feng X., Pan Y., Lin Y., Shang T., Wang Q., Pan J., Wang J., Wang H, & Dong L. (2022). Gankyrin and TIGAR cooperatively accelerate glucose metabolism toward the PPP and TCA cycle in hepatocellular carcinoma. Cancer Science, 113(12), 4151-4164.

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