K8 18 20r cp004

Manufactured by Biosynth

The K8+18 (20R-CP004) is a laboratory instrument designed for analytical and research applications. It is a multi-channel pipette capable of accurately and precisely dispensing liquids within a specified volume range. The core function of this product is to enable reproducible liquid handling in a laboratory setting.

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Lab products found in correlation

Alexa fluor phalloidin, by Thermo Fisher Scientific (1 mentions) Hvin 1, by Merck Group (1 mentions) Vimentin d21h3, by Cell Signaling Technology (1 mentions) β catenin, by Cell Signaling Technology (1 mentions) Ab151733, by Abcam (1 mentions) Ab97959, by Abcam (1 mentions) Phospho nf κb p65 ser536, by Cell Signaling Technology (1 mentions) β actin, by Merck Group (1 mentions) Apc conjugated anti cd44, by BD (1 mentions) Nb100 122, by Novus Biologicals (1 mentions) Pe conjugated anti cd24, by BD (1 mentions) Apc conjugated anti pd l1, by BD (1 mentions) Fitc conjugated anti cd24, by BD (1 mentions) Cav 1, by BD (1 mentions) P53 sc 126, by Santa Cruz Biotechnology (1 mentions) Hif1a, by BD (1 mentions)

2 protocols using k8 18 20r cp004

Immunofluorescence Analysis of Cellular Adhesions

Cited 1 time

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Cells were cultured on various substrates overnight and then fixed with 4% paraformaldehyde (PFA; 2D: 20 min, room temperature; 3D: overnight, 4°C) and stained as described (Levental et al., 2009 (link)). Primary antibodies against paxillin (5H11, Millipore; 610620, BD; P1093, Sigma-Aldrich; 32084, Abcam), phospho–paxillin-tyrosine 118 (44-722G, Life Technologies; 2541, Cell Signaling), phospho–paxillin-tyrosine 31 (44-720F, Life Technologies), vinculin (hVIN-1, Sigma-Aldrich; 700062, Invitrogen; V284, Santa Cruz Biotechnology), phospho–FAK-tyrosine 397 (44-625G, Invitrogen), vimentin (D21H3, Cell Signaling), β-catenin (8480, Cell Signaling), K14 (PRB-115P, Covance), K8+18 (20R-CP004, Fitzgerald Industries), PINCH (EP1944, Abcam), ILK (611803, BD), and Alexa Fluor–phalloidin (Invitrogen) were used. Secondary antibodies used include Alexa Fluor goat anti-mouse, anti-rabbit, and anti-rat (488, 568, and 633 conjugates). For ILK immunofluorescence (IF), cells were fixed in 100% ice-cold methanol.

Mekhdjian A.H., Kai F., Rubashkin M.G., Prahl L.S., Przybyla L.M., McGregor A.L., Bell E.S., Barnes J.M., DuFort C.C., Ou G., Chang A.C., Cassereau L., Tan S.J., Pickup M.W., Lakins J.N., Ye X., Davidson M.W., Lammerding J., Odde D.J., Dunn A.R, & Weaver V.M. (2017). Integrin-mediated traction force enhances paxillin molecular associations and adhesion dynamics that increase the invasiveness of tumor cells into a three-dimensional extracellular matrix. Molecular Biology of the Cell, 28(11), 1467-1488.

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Comprehensive Stemness and EMT Marker Profiling

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COMMD1 (NBP2-4633, Novusbio), ZEB1 (NBP2-13159, Novusbio), Sox2 (ab97959, Abcam), KLF4 (ab151733, Abcam), Oct-4 (2750, Cell Signaling), MYC (5605, Cell Signaling), NANOG (D73G4)(4903, Cell Signaling), K8/18 (20R-Cp004, Fitzgerald Industries), P53 (SC-126, Santa Cruz Biotechnology), Phospho-NFκB p65 (Ser536) (93H1)(3033, Cell Signaling), HIF1A(610959, BD), HIF2A(NB100-122, Novusbio), MCT4(sc-50329, scbt), CAV1(610407, BD), B-actin (A5441, Sigma), tubulin (T4026, Sigma), FITC-conjugated anti-CD24, PE-conjugated anti-CD24, APC-conjugated anti-CD44, APC-conjugated anti-PD-L1 (BD Biosciences).

Lin Z., Roche M.E., Díaz-Barros V., Domingo-Vidal M., Whitaker-Menezes D., Tuluc M., Uppal G., Caro J., Curry J.M, & Martinez-Outschoorn U. (2024). MiR-200c reprograms fibroblasts to recapitulate the phenotype of CAFs in breast cancer progression. Cell Stress, 8, 1-20.

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