To determine the amount of tofacitinib citrate delivered into skin, an extraction study was performed. Following IVPT, skin permeation area was cut out and added to a tube along with 3 mL of methanol. The skin tissue was then homogenized using a bead mill homogenizer (Bead Ruptor 24 Elite by Omni International, Kennesaw, GA, U.S.A) at set parameters (number of cycles: 2, speed: 6.00 m/s, time: 30 s, dwell time: 60 s) followed by 4 h of shaking and then filtered through 0.22 μm syringe filters (Cell treat Scientific Products, Shirley, MA, USA) and analyzed using the HPLC method. Results were reported as mean ± SE (n=4) for each group.
0.22 μm syringe filters
Manufactured by Celltreat
Sourced in United States
The 0.22 μm syringe filters are designed for the filtration of liquid samples. They feature a 0.22 micron pore size membrane that can remove particles, microorganisms, and other contaminants from the sample. The filters are compatible with a variety of syringe sizes and materials.
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Lab products found in correlation
4 protocols using 0.22 μm syringe filters
1
Tofacitinib Skin Permeation Assay
2
Solubility Determination of Tofacitinib
3
Tofacitinib Skin Permeation Assay
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To determine the amount of tofacitinib citrate delivered into skin, an extraction study was performed. Following IVPT, skin permeation area was cut out and added to a tube along with 3 mL of methanol. The skin tissue was then homogenized using a bead mill homogenizer (Bead Ruptor 24 Elite by Omni International, Kennesaw, GA, U.S.A) at set parameters (number of cycles: 2, speed: 6.00 m/s, time: 30 s, dwell time: 60 s) followed by 4 h of shaking and then filtered through 0.22 μm syringe filters (Cell treat Scientific Products, Shirley, MA, USA) and analyzed using the HPLC method. Results were reported as mean ± SE (n=4) for each group.
4
Solubility Determination of Tofacitinib
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Saturation solubility of tofacitinib citrate was determined in 1X PBS solution. To test saturation solubility, excess amount of drug was added to 1mL of solvent followed by overnight shaking at room temperature. After that, the solution was centrifuged at 13,400 rpm for 15 min at 25 °C, filtered through 0.22 μm syringe filters (Cell treat Scientific Products, Shirley, MA, USA), diluted appropriately, and analyzed using HPLC.
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