In animals assigned to the reperfused group (n = 7), a 2.0–2.5 mm angioplasty balloon (Maverick, Boston Scientific, Natick MA, USA) was introduced over a coronary guide wire into the left circumflex (LCX) coronary artery. After determining the proper balloon position for the occlusion, the balloon catheter was inflated and left in position for 90 min to induce MI. After 90 min of ischaemia the balloon was deflated and removed, and coronary angiography was repeated to confirm recanalisation. The femoral artery was decannulated and surgically ligated, and the wound was closed.
Maverick
Manufactured by Boston Scientific
Sourced in United States
The Maverick is a lab equipment product designed for performing various scientific and medical procedures. It serves as a versatile and reliable tool for researchers and medical professionals. The core function of the Maverick is to provide a sturdy and precise platform for conducting laboratory tasks, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
7 protocols using maverick
1
Myocardial Infarction Induction via LCX Occlusion
2
Porcine Model of Myocardial Ischemia
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All procedures were performed with the approval of the local Experimental Animal Care Committee (EK SOI/31/26-11/2014) of the University of Kaposvar, Hungary, conforming to the
Guide for the Care and Use of Laboratory Animals published by the US National Institute of Health (NIH Publication No. 85–23, revised 1996). All animal experiments were conducted at the institute of diagnostic imaging and radiation oncology, University of Kaposvar.
Female domestic pigs received 12mg/kg ketamine, 1mg/kg xylazine and 0.04mg/kg atropine as anaesthesia. The anaesthesia was deepened via mask maintaining 1.5–2.5 vol % isofluran, 1.6–1.8 vol % O
2 and 0.5 vol % N
2O. In total, 200IU/kg of heparin was administrated via the right femoral artery, and selective angiography of LAD arteries was performed prior to induction of myocardial ischemia (MI). MI was induced by 90min balloon occlusion (3.0mm ø, 15mm length, 5atm; Maverick, Boston Scientific, MA, USA) at the mid-part of the LAD artery following balloon deflation. The % O
2 saturation, blood pressure and electrocardiogram were continuously measured during the intervention.
Guide for the Care and Use of Laboratory Animals published by the US National Institute of Health (NIH Publication No. 85–23, revised 1996). All animal experiments were conducted at the institute of diagnostic imaging and radiation oncology, University of Kaposvar.
Female domestic pigs received 12mg/kg ketamine, 1mg/kg xylazine and 0.04mg/kg atropine as anaesthesia. The anaesthesia was deepened via mask maintaining 1.5–2.5 vol % isofluran, 1.6–1.8 vol % O
2 and 0.5 vol % N
2O. In total, 200IU/kg of heparin was administrated via the right femoral artery, and selective angiography of LAD arteries was performed prior to induction of myocardial ischemia (MI). MI was induced by 90min balloon occlusion (3.0mm ø, 15mm length, 5atm; Maverick, Boston Scientific, MA, USA) at the mid-part of the LAD artery following balloon deflation. The % O
2 saturation, blood pressure and electrocardiogram were continuously measured during the intervention.
3
Autologous BM-MSC Delivery for Myocardial Infarction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The injection route of the BM-MSCs has been described elsewhere [16 (link)]. The packed final product of clinical-grade autologous BM-MSC (7.2 ± 0.90 × 107 cells) was gently transferred into the infusion syringe and mixed to minimize cell aggregation before infusion into the LAD via the central lumen of an over-the-wire balloon catheter (Maverick®, Boston Scientific, Natick, MA, USA). To allow the maximum contact time of the BM-MSC with micro-circulation of the LAD infarction territory, the balloon was inflated inside the stent at a low pressure to transiently interrupt antegrade blood flow during the infusions. The entire cell injection was done during three transient occlusions, each lasting 2 to 3 min. Between occlusions, the coronary artery was re-perfused for 3 min. After cell injection, close observation identified clinical changes and/or possible complications. Cardiac enzyme and electrocardiography measurements were repeated to assess periprocedural myocardial infarction (MI). The mean duration of autologous BM-MSC culture from BM aspiration to intracoronary injection was 25.0 ± 2.4 days.
4
Rabbit Iliac Artery Endothelial Denudation and Stent Implantation
5
Transient Hindlimb Hypoperfusion in Rats
6
Percutaneous Closed-Chest Reperfused MI
7
Porcine Model of Acute Myocardial Infarction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All animal studies were approved by the local Experimental Animal Care Committee of University of Kaposvar, Hungary where the experiments were performed (EC 246/002/SOM2006, MAB-28-2005). The experiments conform to the “Position of the American Heart Association on Research.”
Closed-chest, acute reperfused MI was induced in 39 female domestic pigs (23 ± 3 kg) by percutaneous catheter-based balloon occlusion of the left anterior descending coronary artery (LAD), as described previously (Gyongyosi et al., 2011 (link)). Briefly, after direct puncture of the right femoral artery and administration of 200 IU/kg of heparin, a 90-min percutaneous balloon (3.0 mm in diameter × 15 mm long, Maverick, Boston Scientific Corp., Natick, MA, USA) occlusion (5 atm) of the mid LAD, beyond the origin of the second major diagonal branch was performed. The complete occlusion of the artery distal to the balloon was confirmed by angiography. After a 90-min LAD occlusion, the balloon was deflated slowly to allow reperfusion. The puncture site was then closed with Angioseal (St. Jude Medical, St. Paul, MN, USA), and the pigs were allowed to recover.
One-week (8 ± 2 days) post-MI, the pigs were randomized to receive either IC infusion or 3D NOGA-guided percutaneous intramyocardial injections of the GFP-Luc-MSC. The study design with different follow-ups (FUPs) is shown in Figure1 .
Closed-chest, acute reperfused MI was induced in 39 female domestic pigs (23 ± 3 kg) by percutaneous catheter-based balloon occlusion of the left anterior descending coronary artery (LAD), as described previously (Gyongyosi et al., 2011 (link)). Briefly, after direct puncture of the right femoral artery and administration of 200 IU/kg of heparin, a 90-min percutaneous balloon (3.0 mm in diameter × 15 mm long, Maverick, Boston Scientific Corp., Natick, MA, USA) occlusion (5 atm) of the mid LAD, beyond the origin of the second major diagonal branch was performed. The complete occlusion of the artery distal to the balloon was confirmed by angiography. After a 90-min LAD occlusion, the balloon was deflated slowly to allow reperfusion. The puncture site was then closed with Angioseal (St. Jude Medical, St. Paul, MN, USA), and the pigs were allowed to recover.
One-week (8 ± 2 days) post-MI, the pigs were randomized to receive either IC infusion or 3D NOGA-guided percutaneous intramyocardial injections of the GFP-Luc-MSC. The study design with different follow-ups (FUPs) is shown in Figure
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!