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Morphometric system

Manufactured by Nikon
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The Nikon Morphometric System is a comprehensive solution for the analysis and quantification of biological samples. It provides advanced imaging and measurement capabilities for researchers and scientists working in various fields, such as cell biology, developmental biology, and plant science. The system combines high-resolution microscopy with powerful software tools for the accurate measurement and analysis of morphological features of cells, tissues, and organisms.

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Lab products found in correlation

400 fluorescent microscope, by Nikon (6 mentions) Ubiquitin, by Merck Group (5 mentions) Ubiquitin, by Jackson ImmunoResearch (5 mentions) Dapi nuclear stain, by Thermo Fisher Scientific (5 mentions) Alexa fluor 594 donkey anti mouse, by Jackson ImmunoResearch (3 mentions) Alexa fluor 488, by Jackson ImmunoResearch (3 mentions) Alexa fluor 594, by Jackson ImmunoResearch (2 mentions) Rabbit anti 5 hydroxymethylcytosine, by Active Motif (1 mentions) Donkey anti mouse alexa fluor 488, by Jackson ImmunoResearch (1 mentions) Alexa fluor 488 donkey anti rabbit, by Jackson ImmunoResearch (1 mentions) Bcl2 associated x (bax), by Jackson ImmunoResearch (1 mentions) Bcl2 associated x (bax), by Enzo Life Sciences (1 mentions) Bcl2 associated x (bax), by Abcam (1 mentions) Ubiquitin, by Thermo Fisher Scientific (1 mentions)

7 protocols using morphometric system

1

Quantitative Immunofluorescence Analysis of Complement Proteins in FFPE Tissue

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FFPE tissue slides were triple stained for various complements (Rabbit anti-C1q, Bioss, Woburn, MA; Rabbit anti-C3 and Rabbit anti-C5, Abcam, Cambridge, MA; Rabbit anti-C5aR, Novus Biologicals, San Diego, CA) and ubiquitin (Millipore, Temecula CA).
The complements were detected using the second antibody donkey anti-rabbit Alexa Fluor 488 (Jackson Lab, West Grove, PA). ubiquitin was detected using the second antibody donkey anti-mouse Alexa Fluor 594 (Jackson Lab, West Grove, PA). All slides were stained with the nuclear stain DAPI (Molecular Probes, Eugene, OR). The slides were examined with a Nikon 400 fluorescent microscope. The fluorescence intensity of staining of the protein of interest was measured quantitatively using 40× objectives and a standard exposure time of 800 ms using a Nikon 400 fluorescent microscope with three filters (FITC-green, Texas Red, and Tri-Color), and the Nikon morphometric system. The results were displayed as a graph attached to the immunofluorescent photography using a screen snip.
Shen H., French B.A., Liu H., Tillman B.C, & French S.W. (2014). Increased activity of the complement system in the liver of patients with alcoholic hepatitis. Experimental and molecular pathology, 97(3), 338-344.
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2

Quantitative Analysis of DNA Methylation

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FFPE tissue slides were triple stained for 5-mC (mouce anti-5’-methylcytosine (Millipore, Temecula CA); Rabbit anti-5’-hydroxymethylcytosine (Active Motif, Carlsbad, CA).
The slides were examined with a Nikon 400 fluorescent microscope. The fluorescence intensity of staining of the protein of interest was measured quantitatively using 40× objective and a standard exposure time of 800 ms using a Nikon 400 fluorescent microscope with three filters (FITC-green, Texas Red, and Tri-Color), and the Nikon morphometric system. The results were displayed as a graph attached to the immunofluorescent photography using a screen snip.
Shen H., French B.A., Tillman B.C., Li J, & French S.W. (2015). Increased DNA methylation in the livers of patients with alcoholic hepatitis. Experimental and molecular pathology, 99(2), 326-329.
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3

Quantifying Protein Fluorescence Intensity

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The fluorescence staining intensity of the proteins were then measured in 3 different areas on each slide. Using a Nikon 400 fluorescent microscope and the Nikon morphometric system, quantitative measurements were taken with 40× objective magnification and a standard exposure time of 800ms. All photos and measurements were made at the same level of UV light intensity. The results were displayed as a graph attached to the immunofluorescent photography using a screen snip.
MENDOZA A.S., Dorce J., Peng Y., French B.A., Tillman B., Li J, & French S.W. (2014). Levels of Metacaspase1 and Chaperones Related to Protein Quality Control in Alcoholic and Nonalcoholic Steatohepatitis. Experimental and molecular pathology, 98(1), 65-72.
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4

Quantitative Immunofluorescence Analysis of Protein Localization in Liver Biopsy Samples

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FFPE liver biopsy tissue slides were double stained for ubiquitin (Millipore, Temecula, CA) and the second protein as listed in Table 1. ubiquitin was detected using the second antibody donkey anti-mouse Alexa Fluor 594 (Jackson Labs, West Grove, PA), while the other protein was detected using the second antibody donkey anti-rabbit Alexa Fluor 488 or donkey anti-mouse Alexa Fluor 488 (Jackson Labs, West Grove, PA). All slides were stained with the nuclear stain DAPI (Molecular Probes, Eugene, OR). The fluorescence intensity of staining of the protein of interest was measured quantitatively using 40x objective magnification and a standard exposure time of 800 ms using a Nikon 400 fluorescent microscope with three filters (FITC-green, Texas-red, and tricolor), and the Nikon morphometric system. The results were displayed as a graph attached to the immunofluorescent photography using a screen snip. The staining was compared among MDB forming AH hepatocytes, neighboring non-MDB forming AH hepatocytes, and control normal hepatocytes.
Peng Y., French B.A., Tillman B., Morgan T, & French S.W. (2014). The inflammasome in alcoholic hepatitis: its relationship with Mallory-Denk body formation. Experimental and molecular pathology, 97(2), 305-313.
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5

Quantitative Immunofluorescence Analysis of BRCA, Cell Cycle, and Apoptosis Markers

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FFPE tissue slides were double stained for BRCA1, BRCA2, p15, Bax and Tec (Abcam Inc., Cambridge MA) with Ubiquitin (Millipore, Temecula, CA). A second set of slides were stained for BRCA1, BRCA2, p15, Bax and Tec (Enzo Life Sciences, Farmingdale, NY) with Ubiquitin (Millipore, Temecula, CA). BRCA1, BRCA2, p15, Bax and Tec were detected using donkey anti rabbit Alexa Fluor 488 (Jackson Immuno Research Laboratories Inc. West Grove, PA). Ubiquitin was detected using donkey anti mouse Alexa Fluor 594 (Jackson Labs. West Grove, PA). All slides were stained with the nuclear stain DAPI (Molecular Probes, Eugene, OR). The fluorescence intensity of stained protein of interest was measured quantitatively using a 40× objective and a standard exposure time of 800ms using a Nikon 400 fluorescent microscope with three filters (FITC-green, Texas Red, and Tri-Color), and the Nikon morphometric system. The results were displayed as a graph attached to the immunofluorescent photography using a screen snip.
Liu H., Gong M., French B.A., Liao G., Li J., Tillman B, & French S.W. (2015). Aberrant modulation of the BRCA1 and G1/S cell cycle pathways in alcoholic hepatitis patients with Mallory Denk Bodies revealed by RNA sequencing. Oncotarget, 6(40), 42491-42503.
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6

Double Immunofluorescence Staining of Tissue Slides

Cited 1 time
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Formalin fixed, paraffin embedded tissue slides were double stained for SYK (Abcam Inc., Cambridge MA) and Ubiquitin (Millipore, Temecula, CA). A second set of slides were stained for AKT1 (Invitrogen, Camarillo, CA) and Ubiquitin. SYK and AKT1 were detected using the second antibody donkey anti rabbit Alexa fluora 488 and anti-mouse Alexa fluora 594 (Jackson Labs, West Grove, PA) respectively. Ubiquitin was detected using the second antibody donkey anti mouse Alexa Fluor 594 (Jackson Labs. West Grove, PA). All slides were stained with the nuclear stain DAPI (Molecular Probes, Eugene, OR). The fluorescence intensity of stained protein of interest was measured quantitatively using a 40× objective and a standard exposure time of 800ms using a Nikon 400 fluorescent microscope with three filters (FITC-green, Texas Red, and Tri-Color), and the Nikon morphometric system (Liu et al., 2015 (link)).
Afifiyan N., Tillman B., French B., Masouminia M., Samadzadeh S, & French S. (2017). Over expression of Proteins that Alter the Intracellular Signaling Pathways in the Cytoplasm of the Liver Cells Forming Mallory-Denk Bodies. Experimental and molecular pathology, 102(1), 106-114.
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7

Quantitative Analysis of CXCR2 and IL-8 Expression

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FFPE tissue slides were double stained for CXCR2 and IL-8 (Abcam Inc., Cambridge MA) with Ubiquitin (Millipore, Temecula, CA). A second set of slides was stained for CXCR2 and IL-8 (Enzo Life Sciences, Farmingdale, NY) with Ubiquitin (Millipore, Temecula, CA). CXCR2 and IL-8 were detected using donkey anti rabbit Alexa Fluor 488 (Jackson Immuno Research Laboratories Inc. West Grove, PA). Ubiquitin was detected using donkey anti mouse Alexa Fluor 594 (Jackson Labs. West Grove, PA). All slides were stained with the nuclear stain DAPI (Molecular Probes, Eugene, OR). The fluorescence intensity of stained protein of interest was measured quantitatively using a 40× objective and a standard exposure time of 800ms using a Nikon 400 fluorescent microscope with three filters (FITC-green, Texas Red, and Tri-Color), and the Nikon morphometric system.
Liu H., French B.A., Nelson T.J., Li J., Tillman B, & French S.W. (2015). IL-8 signaling is up regulated in alcoholic hepatitis and DDC fed mice with Mallory Denk Bodies (MDBs) present. Experimental and molecular pathology, 99(2), 320-325.
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