Whole cell protein extraction kits

Apigenin (purity >99.5%) was purchased from Shanghai Hitsanns Co., Ltd (Shanghai, China). Kits for alanine aminotransferase (ALT), aspartate aminotransferase (AST), malondialdehyde (MDA), myeloperoxidase (MPO), and glutathione (GSH) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). H2DCFDA, RPMI1640, and fetal bovine serum (FBS) were purchased from Life Technology (Carlsbad, CA, USA). A Pierce BCA Protein Assay Kit was purchased from Thermo Fisher Scientific (Waltham, MA, USA). EX-527 was purchased from Sigma-Aldrich (St. Louis, MO, USA). Whole-cell protein extraction kits and enhanced chemiluminescence kits were obtained from Millipore (Darmstadt, Germany). Antibodies for immunoblotting, including β-actin (#4970), Lamin B (#13435), SIRT1 (#8469), p53 (#2524), ac382-p53 (#2525), NRF2 (#12721), and p65 (#8242) were purchased from Cell Signaling Technology (Danvers, MA, USA; all 1: 1,000 dilutions). Enzyme-linked immunosorbent assay (ELISA) kits were purchased from RapidBio (West Hills, CA, USA). TRIzol reagent was purchased from Life Technology (Carlsbad, CA, USA). PrimeScript RT Master Mix and SYBR Premix Ex Taq were purchased from TaKaRa (Shiga, Japan). APAP, NAPQI, 3-(4,5-dimethyl-thiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT), and other reagents were purchased from Sigma-Aldrich unless otherwise indicated.

FST of 98% purity was purchased from Shanghai Hitsanns Co., Ltd (Shanghai, China). Kits for the analysis of alanine/aspartate aminotransferases (ALT/AST), malondialdehyde (MDA), myeloperoxidase (MPO), and GSH were obtained from Nanjing Jiancheng (Nanjing, China). H2DCFDA, RPMI1640, and fetal bovine serum (FBS) were obtained from Life Technology (Carlsbad, CA, USA). The Pierce^® BCA Protein Assay Kit was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Whole cell protein extraction kits and enhanced chemiluminescence kits were obtained from Millipore (Darmstadt, Germany). Antibodies for immunoblotting including β-actin (#4970), LC3 (#2775), p62 (#88588), ATG5 (#2630), caspase-1 (#3866), IL-1β (#12242) were purchased from Cell Signaling Technology (Danvers, MA, USA) (all 1:1,000 dilutions). Enzyme-linked immunosorbent assay (ELISA) kits were purchased from RapidBio (West Hills, CA, USA). TRIzol reagent was purchased from Life Technology (Carlsbad, CA, USA). PrimeScript^® RT Master Mix and SYBR^® Premix Ex Taq^™ were purchased from Takara (Shiga, Japan). APAP, NAPQI, MTT, and other reagents were obtained from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise indicated.