Cocaine (purity >99%) was purchased from Sigma-Aldrich (CAS; St. Louis, MO, USA). Cell culture reagents were purchased from ScienCell (Carlsbad, CA, USA).
Cell culture reagents
Manufactured by ScienCell
Sourced in United States
Cell culture reagents are a collection of solutions and substances used to support and maintain the growth and viability of cells in a laboratory setting. These reagents provide the necessary nutrients, growth factors, and environmental conditions for cells to thrive and proliferate. They are designed to ensure the optimal growth and health of cultured cells, which is essential for various research and experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Electrophoresis reagents, by Bio-Rad (7 mentions)
Piracetam, by Merck Group (4 mentions)
Goat anti rabbit igg, by Santa Cruz Biotechnology (3 mentions)
Nitrocellulose membrane, by Bio-Rad (3 mentions)
Goat anti mouse igg, by Santa Cruz Biotechnology (2 mentions)
Primary antibodies against hdacs 1 7, by Cell Signaling Technology (2 mentions)
Fluorescent secondary antibody, by Thermo Fisher Scientific (2 mentions)
Cdc25c, by Santa Cruz Biotechnology (1 mentions)
Mouse anti cyclin b, by BD (1 mentions)
Dnmt3b, by Epigentek (1 mentions)
Dnmt1, by Epigentek (1 mentions)
Dnmt3a, by Epigentek (1 mentions)
Mitochondrial isolation kit, by Abcam (1 mentions)
H3k56ac, by Epigentek (1 mentions)
H3k14ac, by Epigentek (1 mentions)
Goat anti mouse igg antibody, by Santa Cruz Biotechnology (1 mentions)
Pgc 1α, by Santa Cruz Biotechnology (1 mentions)
Antibodies against acc, by Cell Signaling Technology (1 mentions)
Mct 1, by Cell Signaling Technology (1 mentions)
Mct 4, by Cell Signaling Technology (1 mentions)
11 protocols using cell culture reagents
1
High-Purity Cocaine Cell Culture
2
Antioxidant Enzyme Regulation in Cell Culture
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Cell culture reagents were purchased from Sciencell (Carlsbad, CA, USA). The glutathione synthetase (GSS), super oxide dismutase (SOD), glutathione peroxide (GPx), and CDC25C antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The mouse anti-cyclin B and CDK-1/CDC-2 antibodies were purchased from BD Transduction Laboratories (San Jose, CA, USA). The goat anti-rabbit IgG and goat anti-mouse IgG antibodies were purchased from Santa Cruz Biotechnology. The propidium iodide was purchased from PD bioscience (San Jose, CA, USA). Electrophoresis reagents were purchased from Bio-Rad (Richmond, CA, USA), and nitrocellulose membranes were purchased from Amersham Scientific, Piscataway, NJ, USA. All other reagents were purchased from Sigma–Aldrich (St. Louis, MO, USA).
3
Mitochondrial Isolation and Epigenetic Analysis
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Cell culture reagents were purchased from ScienCell (Carlsbad, CA, USA). DNMT1, DNMT3a, DNMT3b, TET1, TET2, and TET3 antibodies; goat anti-rabbit IgG and goat anti-mouse IgG antibodies; and kits for analysis of global DNA methylation of 5mC were purchased from Epigentek Group Inc. (Farmingdale, NY, USA). Mitochondrial isolation kits were purchased from Abcam (CA). Electrophoresis reagents were purchased from Bio-Rad (Richmond, CA, USA), and nitrocellulose membranes were purchased from Amersham Scientific (Piscataway, NJ).
4
Histone Modulations in Psychostimulant Abuse
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Psychostimulants–cocaine and METH, Opioid- morphine and piracetam (purity > 99%) were purchased from Sigma-Aldrich (CAS- St. Louis, MO, USA). Cell culture reagents were purchased from ScienCell (Carlsbad, CA, USA). Primary antibodies against HDACs (1–7) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). The HATs PCAF and GCN5 were purchased from Proteintech (Rosemont, IL) and p300 was purchased from Epigentek (Farmingdale, NY). Histone H3 Acetylation Antibody Panel Pack I (H3K9ac, H3K14ac, H3K18ac, H3K27ac) and H3K56ac were purchased from Epigentek (Farmingdale, NY, USA). A polyclonal histone-H3 antibody was purchased from Proteintech (Rosemont, IL). Electrophoresis reagents and nitrocellulose membranes were purchased from Bio-Rad (Richmond, CA, USA). All other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).
5
Cell-based HDAC Expression Analysis
6
Piracetam and Cell Culture Reagents Protocol
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Piracetam (purity > 99%) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Cell culture reagents were purchased from ScienCell (Carlsbad, CA, USA). Primary antibodies were purchased from Proteintech (Rosemont, IL, USA) and EpiGentek (Farmingdale, NY, USA). Electrophoresis reagents and nitrocellulose membranes were purchased from Bio-Rad (Richmond, CA, USA). All other reagents were purchased from Sigma–Aldrich (St. Louis, MO, USA). All fluorescent secondary antibodies were purchased from Thermo Fisher Scientific (Waltham, MA, USA).
7
Piracetam Protein Expression Analysis
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Piracetam (purity > 99%) was purchased from Sigma-Aldrich (CAS- St. Louis, MO, USA). Cell culture reagents were purchased from ScienCell (Carlsbad, CA, USA). Primary antibodies against SIRTs (1–7) were purchased from Proteintech (Rosemont, IL, USA). Electrophoresis reagents and nitrocellulose membranes were purchased from Bio-Rad (Richmond, CA, USA). All other reagents were purchased from Sigma–Aldrich (St. Louis, MO, USA). All fluorescent secondary antibodies were purchased from Thermo Fisher Scientific (Grand Island, NY, USA).
8
Protein Expression and Phosphorylation Assay
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Cell culture reagents were purchased from Sciencell (Carlsbad, CA, USA). The dopamine receptor-2 (DRD-2) and phospho-CaMK IV (The 196) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The mouse anti-CaMK IV antibody was purchased from BD Transduction Laboratories (San Jose, CA, USA). The phospho-CaMK II (The 196) antibodies were purchased from Signalway Antibody (Pearland, TX, USA). The anti-phospho CaMK II (The 196) antibodies were purchased from Phospho Solutions (Aurora, CO, USA). The rabbit polyclonal CREB antibodies where purchased from Abcam (Cambridge, MA, USA). The goat anti-rabbit IgG and goat anti-mouse IgG antibodies were purchased from Santa Cruz Biotechnology. Electrophoresis reagents were purchased from Bio-Rad (Richmond, CA, USA), and nitrocellulose membranes were purchased from Amersham Scientific, Piscataway, NJ. All other reagents were purchased from Sigma–Aldrich (St. Louis, MO, USA).
9
Anti-inflammatory Effects of Crataegus pinnatifida
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Human colonic cancer Caco-2 cells were obtained from the Chinese Academy of Sciences (Shanghai, China) and cell culture reagents were purchased from ScienCell Research Laboratories (San Diego, USA). ELISA kits for IL-6, IL-8, MCP-1, and IL-1β were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Other chemical reagents were purchased from Sigma Chemical. The fruits of Crataegus pinnatifida L. were collected in October 2018 from Qinghe (Hebei Province, China).
10
Comprehensive Cellular Metabolism Profiling
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