Anti il 8 antibody

Manufactured by R&D Systems

Sourced in United States

The Anti-IL-8 antibody is a laboratory reagent used for the detection and quantification of the interleukin-8 (IL-8) protein. IL-8 is a chemokine involved in inflammatory processes. This antibody can be used in various immunoassay techniques to measure IL-8 levels in biological samples.

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Lab products found in correlation

Rtca system, by Agilent Technologies (1 mentions) Cim plate, by Roche (1 mentions) Control igg, by R&D Systems (1 mentions) Recombinant human rankl, by R&D Systems (1 mentions) Reparixin, by Selleck Chemicals (1 mentions) Gsk 872, by Selleck Chemicals (1 mentions) Nec 1, by Selleck Chemicals (1 mentions) Recombinant human il 8 protein, by R&D Systems (1 mentions) Z vad fmk, by Selleck Chemicals (1 mentions) Sp600125, by Selleck Chemicals (1 mentions) Arpe 19, by American Type Culture Collection (1 mentions) Anti il 8, by Cell Signaling Technology (1 mentions) Hrp linked secondary antibody, by Cell Signaling Technology (1 mentions) Cholesterol crystals, by Merck Group (1 mentions) Recombinant human il 1α, by R&D Systems (1 mentions) Anti il 6, by Cell Signaling Technology (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Goat serum, by Merck Group (1 mentions) Lentiviral particles, by OriGene (1 mentions)

6 protocols using anti il 8 antibody

Assessing Cancer Cell Migration

Cited 2 times

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Cancer cell migration was assessed by using the Oris^TM Cell migration kit (Platypus Technology, Madison, WI), as previously described [38 (link)]. MDA-MB-231- fibroblast or macrophage conditioned media (100 μl) with or without 0.1 uM reparixin (MCE, Monmouth Junction, NJ) and anti-IL-8 antibodies (R&D Systems, 30 μg/ml) was added once the cancer cells had attached. Migration and proliferation assays using CIM-plates (Roche, Indianapolis, IN) and the RTCA system (ACEA Bioscience, San Diego, CA) were performed as previously described [47 (link)].

Jin K., Pandey N.B, & Popel A.S. (2017). Crosstalk between stromal components and tumor cells of TNBC via secreted factors enhances tumor growth and metastasis. Oncotarget, 8(36), 60210-60222.

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Osteoclastogenesis Reagents and Protocols

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Tissue culture plastics were supplied by Falcon (Lincoln Park, NJ). All other analytical grade reagents were purchased from Sigma (St. Louis, MO) or Fisher (Springfield, NJ). All tissue culture media and reagents were supplied by Life Technologies, Inc. (Grand Island, NY). Recombinant human RANKL, recombinant murine macrophage colony stimulating factor (CSF-1), recombinant human IL-8, IL-8(1–77), IL-8(6–77) and control IgG were purchased from R&D Systems (Minneapolis, MN). Anti-IL-8 antibodies used in vivo were obtained from R&D Systems.

Kamalakar A., Bendre M.S., Washam C.L., Fowler T.W., Carver A., Dilley J.D., Bracey J.W., Akel N.S., Margulies A.G., Skinner R.A., Swain F.L., Hogue W.R., Montgomery C.O., Lahiji P., Maher J.J., Leitzel K.E., Ali S.M., Lipton A., Nicholas R.W., Gaddy D, & Suva L.J. (2014). Circulating Interleukin-8 levels explain breast cancer osteolysis in mice and humans. Bone, 61, 176-185.

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Blocking IL-8 in PDAC

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To block the effects of IL-8, 5 μg/ml of anti-IL-8 antibody (R&D Systems) were added to PDAC.

Li C., Jiang J.Y., Wang J.M., Sun J., An M.X., Li S., Yan J, & Wang H.Q. (2018). BAG3 regulates stability of IL-8 mRNA via interplay between HuR and miR-4312 in PDACs. Cell Death & Disease, 9(9), 863.

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Molecular Inhibitors for Cell Signaling

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Nec-1, GSK’872, NSA, SP600125, reparixin, Z-vad-fmk and Liprosxtatin-1 were bought from Selleck Chemicals (Houston, TX, USA). Anti-IL-8 antibody and recombinant human IL-8 Protein were bought from R&D Systems.

Wang Y., Zhao M., He S., Luo Y., Zhao Y., Cheng J., Gong Y., Xie J., Wang Y., Hu B., Tian L., Liu X., Li C, & Huang Q. (2019). Necroptosis regulates tumor repopulation after radiotherapy via RIP1/RIP3/MLKL/JNK/IL8 pathway. Journal of Experimental & Clinical Cancer Research : CR, 38, 461.

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IL-1α Stimulation of ARPE-19 Cells

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The human RPE cell line ARPE-19 was purchased from ATCC (Cat#CRL-2302, Manassas, VA, US). DMEM/F-12 HEPES medium (Cat# 11330–057), fetal bovine serum (FBS; Cat# 10438–026) and penicillin-streptomycin (100 U/mL-100 μg/ml; Cat# 15140) were obtained from Life Technologies (Grand Island, NY, US). Recombinant human IL-1α was obtained from R&D Systems (Cat# 200-LA-010, Minneapolis, MN, US). Cholesterol crystals were purchased from Sigma-Aldrich (Cat# C8667, St. Louis, MO, US). Anti-IL-6 (Cat# 12153) and anti-IL-8 (Cat#4970) antibody was obtained from Cell Signaling Technology (Danvers, MA, US). anti-IL-8 antibody (Cat# MAB208) was purchased from R&D Systems (Minneapolis, MN, US). Anti-rabbit (Cat# 7074) and anti-mouse (Cat# 7076), HRP-linked secondary antibodies were obtained from Cell Signaling Technology (Danvers, MA, US).

Tian B., Al-Moujahed A., Bouzika P., Hu Y., Notomi S., Tsoka P., Miller J.W., Lin H, & Vavvas D.G. (2017). Atorvastatin Promotes Phagocytosis and Attenuates Pro-Inflammatory Response in Human Retinal Pigment Epithelial Cells. Scientific Reports, 7, 2329.

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Inhibition of ALDH1A1 in Cell Lysis

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The ALDH1A1 inhibitor CM037 was from ChemDiv Inc. CM037 was dissolved in DMSO (10 mM). CelLytic MT Cell Lysis Reagent, goat serum and Eukitt quick-hardening mounting medium for microscopy, 3 kDa FITC-Dextran, ARA-C and DAPI were from Merck KGaA. Fluoromount aqueous mounting medium was from Thermo Fisher Scientific, Inc. Lentiviral particles were from OriGene Technologies, Inc. Matrix Matrigel (growth factors and phenol red-free) was from Becton Dickinson. Anti-IL-8 antibody from R&D Systems (cat. no. MAB208). Tissue-Tek O.C.T. was from Sakura. Retinoic acid and pan-RAR antagonist (cat. no. AGN 193109) were from Tocris Bioscience.

Ciccone V., Terzuoli E., Ristori E., Filippelli A., Ziche M., Morbidelli L, & Donnini S. (2022). ALDH1A1 overexpression in melanoma cells promotes tumor angiogenesis by activating the IL-8/Notch signaling cascade. International Journal of Molecular Medicine, 50(1), 99.

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