S. aureus strains were grown at 37°C in tryptic soy broth (TSB, Difco) with agitation or on plates of tryptic soy agar (Difco). When necessary, culture media were supplemented with appropriate antibiotics chloramphenicol 10 µg/mL, erythromycin 10 µg/mL (Sigma-Aldrich, during intermediate step in the construction of deletion mutants) or erythromycin 25 µg/mL (NTML mutants), kanamycin 150 µg/mL (Apollo Scientific, overnight cultures) or 50 µg/mL (cultures for microscopy), or a combination of kanamycin (50 µg/mL) with neomycin (50 µg/mL, Apollo Scientific), with 100 µg/mL 5- bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal, VWR), or with 0.1 µM CdCl2 (Sigma-Aldrich). E. coli strains were grown at 37°C in Luria-Bertani broth (LB, Difco) or on LB agar (Difco) supplemented with ampicillin 100 µg/mL (Sigma-Aldrich) when required.
Kanamycin
Manufactured by Apollo Scientific
Sourced in Germany, United Kingdom
Kanamycin is a broad-spectrum antibiotic used in laboratory settings. It functions as an aminoglycoside antibiotic, inhibiting protein synthesis in bacterial cells.
Automatically generated - may contain errors
Lab products found in correlation
Ampicillin, by Apollo Scientific (2 mentions)
Neomycin, by Apollo Scientific (2 mentions)
Erythromycin, by Apollo Scientific (2 mentions)
Ampicillin, by Merck Group (2 mentions)
Ciprofloxacin, by Thermo Fisher Scientific (1 mentions)
Nalidixic acid, by Thermo Fisher Scientific (1 mentions)
Imidazole, by Merck Group (1 mentions)
Trizma base and acid, by Merck Group (1 mentions)
Ultra pure dioxane, by Merck Group (1 mentions)
Amicon centrifugal device, by Merck Group (1 mentions)
Sigmafast protease tablets, by Merck Group (1 mentions)
Ni2 resin, by Merck Group (1 mentions)
Isopropyl β d 1 thiogalactopyranoside (iptg), by Apollo Scientific (1 mentions)
Pagemark tricolor, by Avantor (1 mentions)
Dialysis tubing, by Avantor (1 mentions)
Triton x 100, by Avantor (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Dnase, by Merck Group (1 mentions)
Paraformaldehyde pfa, by Merck Group (1 mentions)
Dapi 4 6 diamidino 2 phenylindole, by Merck Group (1 mentions)
4 protocols using kanamycin
1
Staphylococcus aureus and Escherichia coli Culture
2
Synthesis and Purification of Escherichia coli Peptides
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Escherichia coli strain was purchased from the Coli Genetic Stock Center (BW25113). All peptides except PolB (Thermo Fisher, Hampshire, UK) were synthesized by Fmoc solid-phase synthesis, as previously described [15 (link)]. Ampicillin was purchased from Merck (Darmstadt, Germany), kanamycin from Apollo Scientific (Stockport, UK), ciprofloxacin and nalidixic acid from Thermo Fisher (Hampshire, UK).
3
Immunofluorescence Protein Purification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Imidazole, Trizma base and acid, DNase, SIGMAFAST protease tablets, NaCl, Ni2+-resin, anti-PKP1 antibody, DAPI (4′,6-diamidino-2-phenylindole), paraformaldehyde (PFA), and ultra-pure dioxane were obtained from Sigma (Madrid, Spain). Ampicillin, kanamycin, and isopropyl-β-D-1-thiogalactopyranoside were obtained from Apollo Scientific (Stockport, UK). Dialysis tubing with a molecular weight cut-off of 3500 Da, Triton X-100, TCEP (tris(2-carboxyethyl)phosphine) and the SDS protein marker (PAGEmark Tricolor) were obtained from VWR (Barcelona, Spain). Amicon centrifugal devices with a molecular weight cut-off of 30 kDa were obtained from Millipore (Barcelona, Spain). The rest of the materials were of analytical grade. Water was deionized and purified on a Millipore system.
4
Staphylococcus and Escherichia coli Cultivation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The bacterial strains and plasmids used in this study are listed in Appendix Tables S4 and S5 , respectively. Staphylococcus aureus strains were grown in tryptic soy broth (TSB, Difco) or on tryptic soy agar (TSA, VWR), at 37°C or 30°C with aeration. Escherichia coli strains were cultured in Luria–Bertani broth (VWR) with aeration, or Luria–Bertani agar (VWR) at 37°C or 30°C. The growth medium was supplemented, when required, with 5‐bromo‐4‐chloro‐3‐indolyl β‐D‐galactopyranoside (X‐gal, 100 μg ml−1, Apollo Scientific), isopropyl β‐D‐1‐thiogalactopyranoside (IPTG, 0.1 or 0.5 mM, Apollo Scientific), ampicillin (100 μg ml−1, Apollo Scientific), erythromycin (10 μg ml−1, Apollo Scientific), and/or a combination of kanamycin (50 or 25 μg ml−1, Apollo Scientific) with neomycin (50 or 25 μg ml−1, Apollo Scientific).Plasmids were cloned and propagated in E. coli strains DC10B or DH5α.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!