P s6 ribosomal protein s240 244

Manufactured by Cell Signaling Technology

P-S6 ribosomal protein S240/244 is a laboratory reagent used to detect and quantify the phosphorylation of the S6 ribosomal protein at serine residues 240 and 244. It is a tool for researchers to study cell signaling pathways and protein phosphorylation events.

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Lab products found in correlation

P egfr y1068, by Abcam (1 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions) Insulin, by Merck Group (1 mentions) Gentamycin, by Merck Group (1 mentions) Egf 236 eg 200, by R&D Systems (1 mentions) P met y1234 1235, by Cell Signaling Technology (1 mentions) P 4e bp1 t37 46, by Cell Signaling Technology (1 mentions) Byl719, by Selleck Chemicals (1 mentions) Gdc 0941, by Selleck Chemicals (1 mentions) Shp099, by Selleck Chemicals (1 mentions) P her3y1289, by Cell Signaling Technology (1 mentions) P fgfr y653 654, by Cell Signaling Technology (1 mentions) P erk y204, by Santa Cruz Biotechnology (1 mentions) Hsp90, by Santa Cruz Biotechnology (1 mentions) Pakt s473, by Cell Signaling Technology (1 mentions) α tubulin, by Santa Cruz Biotechnology (1 mentions) S6 ribosomal protein, by Cell Signaling Technology (1 mentions) Pierce ecl western blotting substrate, by Thermo Fisher Scientific (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Fibronectin, by Merck Group (1 mentions)

Spelling variants of this product

S6 ribosomal protein (94 citations) P-S6 ribosomal protein (20 citations)

2 protocols using p s6 ribosomal protein s240 244

Investigating Receptor Tyrosine Kinase Signaling

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Reagents were: Insulin (Sigma, I0516), NEAA (Gibco, 11140050), gentamycin (Sigma, G1272), G418 (OZ Biosciences, GS21000).
Inhibitors were: BYL719 (Selleckchem, S2814), SHP099 (Selleckchem, S8278), GDC-0941 (Selleckchem, S1065), GS-493 (Millipore, 538099). All inhibitors were solved in DMSO.
Growth factors were: NRG1 (396-HB-050), EGF (236-EG-200) and FGF10 (345-FG-025), purchased from R&D Systems.
Antibodies: AKT (Cell Signaling, 9272), p-AKT S473 (Cell Signaling, 4060), p-4E-BP1 T37/46 (Cell Signaling, 2855), ERK (Cell Signaling, 9102), p-HER3 Y1289 (Cell Signaling, 4791), p-MET Y1234/1235 (Cell Signaling, 3077), p-FGFR Y653/654 (Cell Signaling 3471), p-S6 ribosomal protein S240/244 (Cell Signaling, 5364), p-ERK Y204 (Santa Cruz, sc-7383), HSP90 (Santa Cruz, sc-13119), SHP2 (Santa Cruz, sc-280), p-SHP2 Y542 antibody (AbCam, 62322), p-EGFR Y1068 (AbCam, 5644). Secondary rabbit (#111-035-144) and mouse (#115-035-062) antibodies were from Dianova.

Heynen G.J., Lisek K., Vogel R., Wulf-Goldenberg A., Alcaniz J., Montaudon E., Marangoni E, & Birchmeier W. (2022). Targeting SHP2 phosphatase in breast cancer overcomes RTK-mediated resistance to PI3K inhibitors. Breast Cancer Research : BCR, 24, 23.

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Western Blot Analysis of Cellular Proteins

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Cells were harvested in SDS-Triton lysis buffer with protease inhibitors. Protein concentration was measured using Bio-Rad DC Protein assay. Equal amounts of protein were loaded and run on SDS-PAGE and transferred to a PVDF membrane (Millipore). Western blot analyses with the following antibodies were performed: PHD3 (NB100-139, Novus Biologicals), CD70 (CD27 Ligand) (MAB2738, R&D Systems), Integrin β1 (610468, BD Transduction Laboratories), LDHA (#3582, Cell Signaling Technologies), MDH2 (ab181873, Abcam), STAT1 (#9176, Cell Signaling Technologies), Fibronectin (F3648, Sigma), alpha-actinin 4 (ALX-210-356-C050, Enzo Life Sciences), GLUT1 (ab14683, Abcam), glyceraldehyde phosphate dehydrogenase (GAPDH) (5G4-6C5, HyTest), S6 ribosomal protein (#2217, Cell Signaling Technologies), pS6 ribosomal protein S235/236 (#2211, Cell Signaling Technologies), pS6 ribosomal protein S240/244 (#3564, Cell Signaling Technologies), p70 S6 kinase (#2708, Cell Signaling Technologies), p-p70 S6 kinase T389 (#9234, Cell Signaling Technologies), α-tubulin (sc-23948, Santa Cruz Biotechnology), β-actin (AC-74, Sigma-Aldrich), anti-mouse-HRP (DAKO), and anti-rabbit HRP (DAKO). Protein detection was performed using Pierce ECL Western blotting substrate (Thermo Fisher Scientific). β-actin, α-tubulin and GAPDH were used as loading controls.

Miikkulainen P., Högel H., Rantanen K., Suomi T., Kouvonen P., Elo L.L, & Jaakkola P.M. (2017). HIF prolyl hydroxylase PHD3 regulates translational machinery and glucose metabolism in clear cell renal cell carcinoma. Cancer & Metabolism, 5, 5.

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