Anti rap1a rap1b

Manufactured by Cell Signaling Technology

Anti Rap1A/Rap1B is a laboratory product that targets the Rap1A and Rap1B proteins. Rap1A and Rap1B are small GTPases involved in various cellular processes. This product can be used to detect and study the expression and localization of Rap1A and Rap1B in biological samples.

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2 protocols using anti rap1a rap1b

Antibody panel for cell signaling study

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Anti PI3KC2α (#22028‐1‐AP, Proteintech), anti GFP (gift from Emilia Turco, University of Turin, Italy), anti α‐tubulin (#2125, Cell Signaling), anti GAPDH (sc‐47724, Santa Cruz Biotechnology), anti Myc‐tag (#2276, Cell Signaling), anti FAK (#71 433, Cell Signaling), anti p‐FAK (tyr397) (#8556, Cell Signaling), anti p‐FAK (tyr925) (#3284, Cell Signaling), anti Paxillin (#2542, Cell Signaling), anti p‐Paxillin (tyr118) (#69 363, Cell Signaling), anti HA‐tag (# 26 183, Thermofisher), anti R‐RAS (#8446, Cell Signaling), anti RASA3 (#PA5‐30445,Invitrogen), anti Rap1A/Rap1B (#4938, Cell Signaling), anti RAS (#3339, Cell Signaling), and anti Vinculin (#V9131, Sigma).

Li H., Prever L., Hsu M.Y., Lo W., Margaria J.P., De Santis M.C., Zanini C., Forni M., Novelli F., Pece S., Di Fiore P.P., Porporato P.E., Martini M., Belabed H., Nazare M., Haucke V., Gulluni F, & Hirsch E. (2022). Phosphoinositide Conversion Inactivates R‐RAS and Drives Metastases in Breast Cancer. Advanced Science, 9(9), 2103249.

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Protein Expression Analysis by Western Blot

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Protein lysates from cells were extracted in RIPA buffer, with 1 × protease inhibitors cocktail. Equal number of proteins were resolved bySurePAGE™ Plus, Bis–Tris (GenScript, M00725) and transferred onto PVDF membranes. Blots were blocked and incubated with primary antibodies overnight at 4 °C. Then membranes were incubated with the secondary antibody at 37 °C for 1 h. The blots were probed with anti-DHCR24 (Santa Cruz Biotechnology, sc-398938), anti-Rap1A/Rap1B (Cell Signaling Technology, 2399), anti-AKT1 (Santa Cruz Biotechnology, sc-5298), anti-pAKT1-Thr308/309 (Signalway Antibody, 13311), anti-FLAG (ABCAM, ab205606), anti-CYP27A1 (ABCAM, ab126785), GAPDH (TransGen Biotech, HC301-01) were used as the internal control. Densitometry analysis was performed using ImageJ software.

Wang X., Zhong F., Chen T., Wang H., Wang W., Jin H., Li C., Guo X., Liu Y., Zhang Y, & Li B. (2024). Cholesterol neutralized vemurafenib treatment by promoting melanoma stem-like cells via its metabolite 27-hydroxycholesterol. Cellular and Molecular Life Sciences: CMLS, 81(1), 226.

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