Media, sera, and antibiotics for cell culture were from Lonza (Lonza Group Ltd, Basel, Switzerland). Antibodies against phospho‐Ser473PKB/Akt1, ERK, and actin were purchased from Santa Cruz Biotechnology (Santa Cruz Biotechnology, Santa Cruz, CA). Phospho‐Thr202/Tyr204ERK, antibodies were obtained from Cell Signaling Technology (Danvers, MA). Anti‐active + pro Caspase 3 antibody was purchased from Abcam (Cambridge, UK), PKB/Akt antibody was from Millipore (Millipore, Billerica, MA). Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS–PAGE) reagents from Bio‐Rad (Bio‐Rad, Hercules, CA). All the other chemicals were from Sigma–Aldrich (Sigma–Aldrich, St. Louis, MO).
Anti active pro caspase 3 antibody
Manufactured by Abcam
Sourced in United Kingdom
The Anti-active + pro Caspase 3 antibody is a lab equipment product that detects both the active and pro forms of Caspase 3 protein. Caspase 3 is a key enzyme involved in the apoptosis (programmed cell death) pathway. This antibody can be used to monitor Caspase 3 activation during various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Antibiotics for cell culture, by Lonza (1 mentions)
Sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page reagents, by Bio-Rad (1 mentions)
Fluorescein labelled phalloidin, by Merck Group (1 mentions)
P53 mouse mab, by Cell Signaling Technology (1 mentions)
In situ cell death detection kit, by Roche (1 mentions)
Pe annexin 5 apoptosis detection kit 1, by BD (1 mentions)
2 protocols using anti active pro caspase 3 antibody
1
Western Blot Analysis of Cell Signaling
2
Antibodies and Reagents for Cell Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The antibodies and reagents used in the present study are listed with their sources in parentheses: mouse monoclonal antibody (mAb) to glyceraldehyde-phosphate dehydrogenase (GAPDH), rabbit polyclonal antibody to Angptl3, integrin alpha 3, total integrin beta 1, phospho-integrin beta 1 (phospho T789) and ILK; anti-active + pro- caspase 3 antibody (Abcam, Cambridge, UK); p53 mouse mAb (Cell Signaling Technology, Beverly, USA); recombinant mouse Angptl3 protein (rm-Angptl3) (R&D Systems, Minneapolis, USA); PE Annexin V Apoptosis Detection Kit I (BD Biosciences, San Diego, USA); In Situ Cell Death Detection kit (Roche, Mannheim, Germany); and fluorescein-labelled phalloidin (Sigma Aldrich, St. Louis, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!