Mircury lna mirna inhibitor

Manufactured by Qiagen

Sourced in Germany

The MiRCURY LNA miRNA inhibitor is a laboratory equipment product designed for the detection and analysis of microRNA (miRNA) expression. It utilizes Locked Nucleic Acid (LNA) technology to provide specific and efficient inhibition of miRNA targets.

Automatically generated - may contain errors

Lab products found in correlation

Hiperfect transfection reagent, by Qiagen (3 mentions) Mircury lna mirna mimic, by Qiagen (3 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (3 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Purefection transfection reagent, by System Biosciences (1 mentions) Cli 095, by InvivoGen (1 mentions) Bay 11 7085, by Cayman Chemical (1 mentions) Ym00479902, by Qiagen (1 mentions) Yi00199006, by Qiagen (1 mentions) Novex tbe running buffer, by Thermo Fisher Scientific (1 mentions) Novex tbe gel, by Thermo Fisher Scientific (1 mentions) Hank s balanced salt solution, by Thermo Fisher Scientific (1 mentions) Dulbecco s phosphate buffered saline, by Thermo Fisher Scientific (1 mentions) Has mir 27b, by Qiagen (1 mentions) Opti mem reduced serum media, by Thermo Fisher Scientific (1 mentions) Pre mir mirna precursor, by Thermo Fisher Scientific (1 mentions) Lipofectamine rnaimax, by Qiagen (1 mentions) X tremegene sirna transfection reagent, by Roche (1 mentions) Tdtomato c1 vector, by Addgene (1 mentions) Nebuilder hifi dna assembly technology, by New England Biolabs (1 mentions)

15 protocols using mircury lna mirna inhibitor

Immortalized Human Fibroblast Culture Protocol

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immortalized human fibroblasts (huFib, Inscreenex, Braunschweig, Germany) were cultured at 37 °C with 5% CO₂ in huFib medium (Inscreenex) [17 (link)]. Twenty-four hours before adding the stimulation reagent for a specific duration, cells were maintained under serum-free conditions (Serum-Free huFib Medium, Inscreenex). Individual experiments were performed at different passages (p21–p35) of the cell line under serum-free conditions on collagen coated cell culture dishes. Each experiment (n) was performed independently if not otherwise stated.
TLR-4 inhibitor (1 µM) (CLI-095, Invivogen, San Diego, CA, USA) or NFκB inhibitor (10 nM) (Bay11-7085, Cayman Chemical, Ann Arbor, MI, USA) were added to the serum-free medium 18 h prior to and concurrently together with the stimulation reagent, as mentioned below.
Transfection of fibroblasts with miRNA-146 mimics (Qiagen, Hilden, Germany) or antimiRNA-146 (miRCURY LNA miRNA Inhibitors, Qiagen) was conducted using Purefection transfection reagent (System Biosciences, Palo Alto, CA, USA) according to the manufacturer’s instructions.

Yogeswaran A., Troidl C., McNamara J.W., Wilhelm J., Truschel T., Widmann L., Aslam M., Hamm C.W., Sadayappan S, & Lipps C. (2021). The C0-C1f Region of Cardiac Myosin Binding Protein-C Induces Pro-Inflammatory Responses in Fibroblasts via TLR4 Signaling. Cells, 10(6), 1326.

+ Open protocol

+ Expand

Knockdown of miR-144-5p and miR-21-5p in Diabetic Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

To knockdown miR-144-5p and miR-21-5p, we injected antagomir-144-5p (Ag-144-5p) and antagomir-21-5p (Ag-21-5p) re-suspended with PBS solution into the tail vein of diabetic mice. We started the treatment 1 wk after inducing diabetes in the mice using STZ injections. At this time, all mice had developed hyperglycemia. The dosage used was 10 mg/kg of body weight, and the antagomirs treatments were administered once a week. The injections were continued until the mice reached 14 wk of age, which marked the end of the experiment. As a comparison, the control groups of diabetic and non-diabetic mice received a scrambled antagomir treatment, as a negative control (NC). A NC is defined to have no hits of >70% homology to any sequence in any organism in the National Center for Biotechnology Information (NCBI) and miRbase databases. It serves as a crucial control to account for any non-specific effects in our experimental setup. All antagomirs (miRCURY LNA miRNA Inhibitors) were obtained from Qiagen and prepared following the manufacturer’s instructions. Notably, upon the administration of antagomiR-21-5p, we observed an immediate and transient lethargic response in mice, lasting for approximately 10 minutes, although they subsequently recovered.

Daamouch S., Blüher M., Vázquez D.C., Hackl M., Hofbauer L.C, & Rauner M. (2024). MiR-144-5p and miR-21-5p do not drive bone disease in a mouse model of type 1 diabetes mellitus. JBMR Plus, 8(5), ziae036.

+ Open protocol

+ Expand

Transfection of miRNA mimics and inhibitors in hESCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hsa-miR mimics (339173, miRCURY LNA miRNA Mimic) and inhibitors (339121, miRCURY LNA miRNA Inhibitors) were ordered from Qiagen, Hilden, Germany. Additionally, negative control mimics (YM00479902, Negative Control miRCURY LNA miRNA Mimic) and negative control inhibitors (YI00199006, Negative control A), which both have no homology to any known miRNA or mRNA in human, were ordered from Qiagen, Hilden, Germany. The following miRNAs were labeled with a fluorescent 5′-carboxyfluorescein (FAM) tag: hsa-miR-19a mimic + inhibitor, hsa-miR-19b mimic + inhibitor, negative control A mimic, negative control inhibitor. Thirty thousand decidualized or non-decidualized hESCs were cultured in single wells of a 48 wells plate and transfected with 25 nM miRNA mimic or negative control mimic. Alternatively, 30 000 hESCs were transfected with 150 nM miRNA inhibitor or negative control inhibitor. All transfections were performed in D- or C-medium by using HiPerFect Transfection Reagent according to manufacturer’s instructions (301704, Qiagen, Hilden, Germany). After transfection for 24 h, hESCs were washed with PBS and transfection efficiency was determined by calculating the percentage of fluorescently labeled hESCs from the total number of hESCs per image.

Berkhout R.P., Keijser R., Repping S., Lambalk C.B., Afink G.B., Mastenbroek S, & Hamer G. (2020). High-quality human preimplantation embryos stimulate endometrial stromal cell migration via secretion of microRNA hsa-miR-320a. Human Reproduction (Oxford, England), 35(8), 1797-1807.

+ Open protocol

+ Expand

Denaturing Gel Electrophoresis of miRNA

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dulbecco’s phosphate-buffered saline (dPBS, 2.6 mMKCl, 1.47 mM KH₂ PO₄, 137 mMNaCl, and 8.05 mM Na₂HPO₄), Hanks’ Balanced Salt Solution (HBSS--,no calcium, no magnesium, refered as HBSS) were obtained from Thermo Fisher Scientific (Waltham, MA). Invitrogen Novex™ TBE-Urea Gels, 15%, 10 well, and TBE-Urea Sample Buffer (2X), for denaturing conditions, and Novex™ TBE Running Buffer (5X), and Novex TBE Gels, 4–20% (non-denaturing conditions) were obtained from Thermo Fisher Scientific. Gel Loading Dye, purple (6X), without SDS was obtained from New England Biolabs (Ipswich, Massachusetts). Five hundred nanometer streptavidin beads were purchased from Bangs Laboratories (Fishers, IN). MiRCURY LNA miRNA Inhibitors (antimiRs) were obtained from Qiagen (Germantown, MD).

Oliveira GP J.r., Barbosa R.H., Thompson L., Pinckney B., Murphy-Thornley M., Lu S., Jones J., Hansen C.H., Tigges J., Wong W.P, & Ghiran I.C. (2021). Electrophoretic mobility shift as a molecular beacon-based readout for miRNA detection. Biosensors & bioelectronics, 189, 113307.

+ Open protocol

+ Expand

miRNA Modulation in Colorectal Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols

miRCURY LNA miRNA mimics (has-miR-23b, Product No. 472291-001; has-miR-27b, Product No. 470553-001) and mimic negative control were obtained from Exiqon (Vedbaek, Denmark). mirVana miRNA mimic (has-miR-24-3p; MC10737) was obtained from Ambion (Austin, TX, USA). miRCURY LNA miRNA inhibitors (has-miR-23b, Product No. 4102299; has-miR-27b, Product No. 4103307; has-miR-24-3p, Product No. 4101706; negative control A) were obtained from Exiqon. HCT116, DLD1, RKO, and HT-29 cells were transfected with miRNA mimics or inhibitors at 10 nM using Lipofectamine RNAiMAX (Thermo Fisher Scientific, Waltham, MA, USA).

Nishida K., Kuwano Y, & Rokutan K. (2020). The MicroRNA-23b/27b/24 Cluster Facilitates Colon Cancer Cell Migration by Targeting FOXP2. Cancers, 12(1), 174.

+ Open protocol

+ Expand

MicroRNA Regulation of Insulin Secretion

Check if the same lab product or an alternative is used in the 5 most similar protocols

INS-1 832/13 cells were seeded (300,000 cells per well) in a 24-well plate with 1 mL/well complete RPMI 1640 medium without antibiotics a day before transfection. Cells were transfected with mature miRNAs called PremiR™ miRNA Precursor from Life Technologies (CA, USA): PremiR-Scramble (AM17110), Premir-130a (PM105106), Premir-130b (PM10777), Premir-152 (PM12269), or miRcury LNA miRNA inhibitors from Exiqon (Denmark): LNA Scramble (#199005-00), LNA130a (#4102212-001), LNA130b (#4102260-001) and LNA152 (#4103524-001) or after reaching ≈60% confluence using Lipofectamine RNAiMAX. A final transfection volume of 600 μL per well contained 50 nM of Pre-miR or LNA in Opti-MEM reduced serum media and 1.5 μL of Lipofectamine RNAiMAX (Life Technologies, CA, USA). In cells transfected with combined Pre-miR-152 and Pre-miR-130a, the concentration of each Pre-miR was reduced to 25 nM. After 6 h transfection, 500 μL of complete RPMI 1640 medium without antibiotics were added to each well. Medium was changed to complete RPM1 1640 with antibiotics after 24 hours of transfection. Cells were assayed for insulin secretion after reaching ≈100% confluence, while protein and RNA samples were extracted from replicate wells at the same time, 72 h post-transfection.

Ofori J.K., Salunkhe V.A., Bagge A., Vishnu N., Nagao M., Mulder H., Wollheim C.B., Eliasson L, & Esguerra J.L. (2017). Elevated miR-130a/miR130b/miR-152 expression reduces intracellular ATP levels in the pancreatic beta cell. Scientific Reports, 7, 44986.

+ Open protocol

+ Expand

miRNA Inhibitor Transfection in C666-1

Check if the same lab product or an alternative is used in the 5 most similar protocols

The miRCURY LNA™ miRNA Inhibitor [consisting of an miR-19b inhibitor and a negative control (NC)] was obtained from Qiagen, Inc. The sequences of the miRNA are proprietary information. The miRNAs were transiently transfected into C666-1 cells at a working concentration of 15 µM using X-tremeGENE siRNA Transfection Reagent (Roche Diagnostics) following the manufacturer's protocol.

Bian L.H., Duan J.L., Zhou C., Shen G.W., Wang X.Y., Yang Y., Zhang X.L, & Xiao S.J. (2020). MicroRNA-19b inhibitors can attenuate the STAT3 signaling pathway in NPC C666-1 cells. Molecular Medicine Reports, 22(1), 51-56.

+ Open protocol

+ Expand

Cloning of SunTag-FL2 Construct

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

For cloning of the SunTag-FL2 construct, we used the SINAPs plasmid from the Singer lab (Addgene #84561)⁴⁴ and a tdTomato-FL2 (containing the 3’UTR of FL2) plasmid previously cloned in-house using the tdTomato-C1 vector (Addgene #54653), a human FL2 clone in pANT7_cGST (DNASU, Arizona State University, Tempe, AZ, clone: HsCD00403041) and a human FL2 3’UTR construct (Switchgear Genomics #S811553). The Ubc promoter, flag tag and SunTag reporter were cloned from the SINAPs construct to replace the CMV promoter and tdTomato reporter of the tdTomato-FL2 plasmid using NEBuilder HiFi DNA Assembly technology (NEB).
The plasmids used for IMP RBP overexpression, GFP-ZBP1^{33 (link)}, GFP-IGF2BP2 and GFP-IGF2BP2 KH3 mutant^{39 (link)}, were from the Singer laboratory. The Lin28 plasmids were purchased from Addgene; Lin28A (#51371), Lin28B (#51373) and Lin28A-mCCHC (#51372). The miRNA let-7 inhibitor sequence (3’-CUCCAUCAUCCAACAU-5’) was previously validated for broad let-7 family inhibition (Frost & Olson, 2011). The custom miRCURY LNA miRNA inhibitor was purchased from Qiagen (Cat num 339146).

Birnbaum R., Biswas J., Singer R.H, & Sharp D.J. (2023). mRNA Localization and Local Translation of the Microtubule Severing Enzyme, Fidgetin-Like 2, in Polarization, Migration and Outgrowth. bioRxiv.

+ Open protocol

+ Expand

Modulating B Cell Receptor Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cells were electroporated using the Neon Transfection System (Thermo Fisher Scientific) with an artificial miR-29c (MISSION miRNA Mimic, 1000 nM) or control short RNA (MISSION miRNA Mimic Negative Control, 1000 nM; Sigma Aldrich), siRNA against TRAF4 (Silencer Select Pre-Designed siRNA, 500 nM) or control siRNA (Silencer Select Negative Control No.1, 500 nM; Thermo Fisher Scientific), LNA miR-29 inhibitor (miRCURY LNA miRNA inhibitor, 500 nM) or control miRNA inhibitor (Negative Control A, 500 nM, Qiagen). The cells were harvested for viability analyses, qRT-PCR and immunoblotting (supplemental Methods). Transfected cells were stimulated with recombinant soluble CD40 ligand (CD40L, 1 µg/ml; Peprotech) in serum-free media (37°C) for the indicated time period and lysed for immunoblotting (see supplemental Methods). For BCR activation by bead-bound anti-IgM, cells were incubated with Dynabeads M-270-Epoxy (Thermo Fisher Scientific) coated with goat F(ab’)2 anti-human IgM or isotype control (see supplemental Methods). BCR crosslinking for the measurement of intracellular calcium flux was performed by soluble goat F(ab’)2 anti-human IgM (Southern Biotechnology; 10 µg/ml), as described elsewhere^{18 (link),19 (link)} (see supplemental Methods).

Sharma S., Pavlasova G.M., Seda V., Cerna K.A., Vojackova E., Filip D., Ondrisova L., Sandova V., Kostalova L., Zeni P.F., Borsky M., Oppelt J., Liskova K., Kren L., Janikova A., Pospisilova S., Fernandes S.M., Shehata M., Rassenti L.Z., Jaeger U., Doubek M., Davids M.S., Brown J.R., Mayer J., Kipps T.J, & Mraz M. (2021). miR-29 modulates CD40 signaling in chronic lymphocytic leukemia by targeting TRAF4: an axis affected by BCR inhibitors. Blood, 137(18), 2481-2494.

+ Open protocol

+ Expand

miR-500a-5p Silencing and Cytokine Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hsa_miR-500a-5p was silenced through the use of miRCURY LNA™ miRNA inhibitor (Qiagen). 400’000 cells, after o.n. incubation, were silenced with 25 pmol of miRNA inhibitor and 3 µl of HiPerFect Transfection Reagent (Qiagen) for 24 hours. Then, the medium was replaced, and cells treated in the presence or absence of G (0.1 µg/mL) for 1 hour, and then stimulated with PMA and ionomycin for 72 hours. Following treatment, cells were centrifuged at 1300 rpm for 5 minutes and supernatants collected for IFN-γ measurement.

Maddalon A., Iulini M., Galbiati V., Colosio C., Mandić-Rajčević S, & Corsini E. (2022). Direct Effects of Glyphosate on In Vitro T Helper Cell Differentiation and Cytokine Production. Frontiers in Immunology, 13, 854837.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!