External platinumcounter electrode

Manufactured by Metrohm

Sourced in United Kingdom

The external platinum counter electrode is a lab equipment component used to provide a reference potential in electrochemical measurements. It is made of platinum and is typically used in conjunction with other electrodes to complete an electrochemical cell.

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Lab products found in correlation

Palmsens4 potentiostat, by PalmSens (1 mentions) Pstrace software, by PalmSens (1 mentions)

2 protocols using external platinumcounter electrode

Electrochemical Biosensor for Antibiotic Resistance Gene Detection

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All electrochemical measurements were undertaken using an Autolab
PGSTAT128N potentiostat with the additional FRA32M electrochemical
impedance spectroscopy module, by scripts written in the Nova 2.1
software package (Metrohm Autolab). PGEs of a 2 mm diameter were purchased
from IJ Cambria Scientific Ltd. (Llanelli, UK). An external platinum
counter electrode (Metrohm, Runcorn, UK) and a Ag/AgCl 3 M KCl reference
electrode (Cole-Parmer, UK) completed the electrochemical cell. Here,
immobilized probes, and capture strands are primer sequences for the
amplification of a region of an artificial plasmid attributing to
the blaOXA-1 β-lactamase gene; encoding extended-spectrum
β-lactamases (ESBLs) and resistance to Oxacillin, across a host
of gram-negative species. This blaOXA-1 β-lactamase
gene sequence (115 nt OXA fragment) serves as the complementary target
sequence in this study. All oligonucleotides for sensor construction
were sourced from Sigma Aldrich (Dorset, UK) (given in Supporting
Information Table S1). 3-Mercapto-1-propanol
(MCP) and all other chemicals required in this study were obtained
from Sigma Aldrich (Dorset, UK). Buffers required in this work are
detailed in Supporting Information Table S2. Details of electrode preparation, functionalization, electrochemical
measurement, and target incubation are available in the Supporting Information.

Williamson P., Piskunen P., Ijäs H., Butterworth A., Linko V, & Corrigan D.K. (2023). Signal Amplification in Electrochemical DNA Biosensors Using Target-Capturing DNA Origami Tiles. ACS Sensors, 8(4), 1471-1480.

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Cleaning and Electrochemical Activation of Multichannel PCB Electrodes

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SEP1 BIOTIP multichannel electrode PCB platform (biotip ltd, Bath, UK) were cleaned according to the supplied protocol. This consisted of a 15-minute submersion in a solution of 50 mM KOH in H2O2 30 % (v/v) at room temperature. The PCB was then rinsed with DI water and dried using compressed air. The PCB was then electrochemically cleaned by submerging in 50 mM KOH (DI water as solvent) with an external platinum counter electrode (Metrohm, Runcorn, UK) and 3M NaCl Ag/AgCl reference electrode (IJ Cambria, Llanelli, UK). Cyclic voltammetry was performed on all working electrodes on the PCB using the following parameters: potential window was -1.2 to 0.6 V, scan rate of 0.1 V/s and 15 scans per electrode. The PCB was then rinsed with DI water and dried again using compressed air. All electrochemical measurements were performed using a PalmSens4 potentiostat and the accompanying PSTrace software, both supplied by Palmsens BV (Houten, Netherlands).

Vezza V., Butterworth A., Lasserre P., Blair E.O., MacDonald A., Hannah S., Rinaldi C., Hoskisson P.A., Ward A.C., Longmuir A., Setford S., Murphy M.E., & Corrigan D.K. (2020). An uncomplicated electrochemical sensor combining a perfluorocarbon SAM and ACE2 as the bio-recognition element to sensitively and specifically detect SARS-CoV-2 in complex samples.

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