Rabbit pcna abs

Manufactured by Santa Cruz Biotechnology

Sourced in United States, United Kingdom

Rabbit PCNA Abs is a research-grade antibody product developed by Santa Cruz Biotechnology. The antibody targets proliferating cell nuclear antigen (PCNA), a protein involved in DNA replication and repair processes. This product is intended for use in various immunological techniques to detect and study PCNA expression in biological samples.

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Lab products found in correlation

Rabbit β tubulin ab6046, by Abcam (2 mentions) Phospho nf κb p65 ser536 93h1, by Cell Signaling Technology (1 mentions)

2 protocols using rabbit pcna abs

Comprehensive Immunoblot Analysis Protocol

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The following primary antibodies were used: mouse GAPDH (ab9484), rabbit β-tubulin (ab6046) from Abcam (Cambridge, UK); rabbit phospho-Akt Ser473 (D9E XP), phospho-p38 MAPK (T180/Y182), phospho-STAT1 (Tyr701) (58D6), phospho-TAK1 (T184/187) (90C7), phospho-JNK (81E11) (T183/Y185), phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204), IκBα (44D4), IRAK1 (D51G7), MyD88 (D80F5), Histone H3 (3H1), and phospho-NF-κB p65 (Ser536) (93H1) from Cell Signaling Technology (Danvers, MA, USA); rabbit PCNA Abs were from Santa Cruz Biotech (Santa Cruz, CA, USA); sheep IRF5 and IRAK4 were from MRC-PPU Reagents (University of Dundee, Dundee, UK); goat TIRAP polyclonal Abs were from Invitrogen (#PA5-18439, Waltham, MA, USA), and mouse STAT1 antibodies were from BD Biosciences (#610185, Wokingham, UK). Secondary antibodies (HRP-linked) were from DAKO Denmark A/S (Glostrup, Denmark).

Nilsen K.E., Skjesol A., Frengen Kojen J., Espevik T., Stenvik J, & Yurchenko M. (2022). TIRAP/Mal Positively Regulates TLR8-Mediated Signaling via IRF5 in Human Cells. Biomedicines, 10(7), 1476.

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Antibody Characterization and Detection

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The following primary antibodies were used for Western blotting: rabbit anti–TICAM-2/TRAM (GTX112785) from Genetex; rabbit mAb anti–human SLAMF1 (10837-R008-50) from Sino Biological Inc.; rabbit β-tubulin (ab6046) from Abcam; phospho-p38 MAPK (T180/Y182), phospho-STAT1 (Tyr701) (58D6), phospho-TAK1 (T184/187) (90C7), IRAK1 (D51G7), MyD88 (D80F5), phospho–IkB-α (14D4), phospho-TBK1/NAK (Ser172; D52C2), anti-DYKDDDDK tag (D6W5B)/Flag tag from Cell Signaling Technology; Living Colors rabbit anti–Full-Length GFP polyclonal antibodies (632592) from Takara Bio Inc.; rabbit PCNA Abs were from Santa Cruz Biotech (Santa Cruz); sheep IRAK1 (used for IPs) and IRAK4 (Used for WB and IPs) were from MRC-PPU Reagents (University of Dundee, Dundee, UK); rabbit anti-HA tag polyclonal Abs (#71-5500), goat TIRAP polyclonal Abs (#PA5-18439) were from Invitrogen. Secondary antibodies (HRP-linked) were from DAKO Denmark A/S. Antibodies used for confocal microscopy—rabbit anti–TICAM-2 from Genetex, recombinant rabbit anti-RAB11-FIP2 antibody (EPR12294-85) (ab180504) from Abcam. Secondary antibodies for confocal microscopy were Alexa Fluor 647 conjugate (A-21235) from Thermo Fisher Scientific.

Nilsen K.E., Zhang B., Skjesol A., Ryan L., Vagle H., Bøe M.H., Orning P., Kim H., Bakke S.S., Elamurugan K., Mestvedt I.B., Stenvik J., Husebye H., Lien E., Espevik T, & Yurchenko M. (2023). Peptide derived from SLAMF1 prevents TLR4-mediated inflammation in vitro and in vivo. Life Science Alliance, 6(12), e202302164.

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