Chemicals were from Sigma-Aldrich unless otherwise specified. SERVAGel™ TG PRiME™ 8–16 % precast gels were from Serva. Primers were synthetized by Eurofins and Integrated DNA Technologies. Monoclonal anti-HA antibody was from Sigma-Aldrich. Secondary antibodies goat anti-mouse IgG DyLight 800 conjugate and goat anti-rabbit IgG DyLight 680 conjugate were from Thermo-Fisher (SA5-35571, 35568, respectively).
Servagel tg prime 8 16 precast gels
Manufactured by Serva Electrophoresis
SERVAGel™ TG PRiME™ 8–16 % precast gels are electrophoresis gels designed for protein separation. They have a gradient concentration of 8-16% polyacrylamide, which allows for the separation of a wide range of protein molecular weights.
Automatically generated - may contain errors
Lab products found in correlation
Goat anti rabbit igg dylight 680 conjugate, by Thermo Fisher Scientific (3 mentions)
Goat anti mouse igg dylight 800 conjugate, by Thermo Fisher Scientific (3 mentions)
Anti flag m2 monoclonal antibody, by Merck Group (2 mentions)
Para benzophenylalanine, by Bachem (2 mentions)
Anti ha monoclonal antibody, by Merck Group (1 mentions)
Serva blue g, by Serva Electrophoresis (1 mentions)
3xflag peptide, by Merck Group (1 mentions)
Streptavidin dylight 800, by Thermo Fisher Scientific (1 mentions)
M2 anti flag agarose beads, by Merck Group (1 mentions)
3 protocols using servagel tg prime 8 16 precast gels
1
Immunoblotting of HA-tagged proteins
2
Protein Expression and Detection
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Chemicals were from Sigma-Aldrich unless otherwise specified, para-benzophenylalanine was from Bachem. The radioactive [S35]-methionine was obtained from Hartman Analytics. SERVAGel™ TG PRiME™ 8–16% precast gels were from Serva. Primers are listed in Supplementary Data 3 and were synthetized by Eurofins and Integrated DNA Technologies. Monoclonal M2 anti-FLAG antibody was from Sigma-Aldrich (F3165). Secondary antibodies goat anti-mouse IgG DyLight 800 conjugate and goat anti-rabbit IgG DyLight 680 conjugate were from Thermo-Fisher (SA5-35571, 35568, respectively).
3
Affinity Purification of Protein Complexes
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Chemicals were from Sigma-Aldrich unless otherwise specified. Detergent n-dodecyl-maltoside (DDM) was from Affimetrix-Anatrace. para-benzophenylalanine was from Bachem. SERVA Blue G and SERVAGel TG PRiME 8–16% precast gels were from Serva. NativePAGE Novex Bis-Tris 3–12% gels were from Life Technologies. Primers are listed in S5 Table and were synthetized by Eurofins and Integrated DNA Technologies. Polyclonal rabbit anti-MBP antibody were from New England Biolabs. Monoclonal mouse anti-RNApol antibody was from BioLegend. Monoclonal M2 anti-FLAG antibody, M2 anti-FLAG agarose beads, and 3xFLAG peptide were from Sigma-Aldrich. CaptureSelect-biotin, Streptavidin DyLight 800, and secondary antibodies goat anti-mouse IgG DyLight 800 conjugate and goat anti-rabbit IgG DyLight 680 conjugate were from Thermo-Fisher.
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