Total protein extracted from HEK 293T cells was, respectively, loaded onto a polyacrylamide gel and analysed by Western blot, with an equal amount (20 μg) per lane. Blots were subsequently detected with the following antibodies: HSP60 (D6F1) XP® Rabbit mAb (Cell Signaling Technology), Anti‐TFAM Rabbit pAb (ZEN BIO), Goat anti‐Rabbit IgG (H&L; ZEN BIO), Mouse Anti‐β actin mAb (ZSGB‐BIO), Goat Anti‐Mouse IgG Secondary Antibody (Sino Biological). Protein bands were detected using an ECL analysis system.
Mouse anti β actin mab
Manufactured by ZSGB-BIO
Sourced in China
Mouse Anti‐β actin mAb is a monoclonal antibody that specifically recognizes the β-actin protein, a highly conserved cytoskeletal protein found in all eukaryotic cells. This antibody can be used to detect and quantify the expression levels of β-actin in various samples, such as cell lysates or tissue extracts, through techniques like Western blotting, immunohistochemistry, or immunocytochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Goat anti rabbit igg h l, by ZenBio (1 mentions)
Dimethyl sulfoxide (dmso), by Solarbio (1 mentions)
Olaparib, by MedChemExpress (1 mentions)
Hrp conjugated secondary antibody, by Dingguo (1 mentions)
Rabbit anti tlr4 antibody, by Abcam (1 mentions)
Ecl plus reagent, by Solarbio (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
4 protocols using mouse anti β actin mab
1
Protein Expression Analysis in HEK 293T Cells
2
Immunofluorescence Analysis of DNA Repair Proteins
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Antibodies we used are as follows: Secondary antibodies: goat anti‐rabbit (ZB-2301) and rabbit anti‐mouse (ZB-2305) secondary antibodies, Alexa Fluor 594-labeled goat anti-mouse (ZF-0513) and Alexa Fluor 488-labeled goat anti-rabbit antibodies (ZF-0511) were purchased from ZSGB-BIO. Anti-RAD51 antibody (Abcam; ab133534), FOXM1 (D12D5) XP® Rabbit mAb (Cell Signaling; #5436), Anti-gammaH2A.X (phospho S139) antibody (Abcam; ab26350), Mouse Anti-βactin mAb (ZSGB-BIO; TA-09).
Pharmacological agents: Olaparib (MedChemExpress; HY-10162), DMSO (Solarbio; D8371).
Plasmid DNA was isolated by plasmid DNA extraction kits (CWBIO; CW2105S) using the alkaline lysis method.
Pharmacological agents: Olaparib (MedChemExpress; HY-10162), DMSO (Solarbio; D8371).
Plasmid DNA was isolated by plasmid DNA extraction kits (CWBIO; CW2105S) using the alkaline lysis method.
3
Western Blot Detection of Anti-p21-Ras scFv
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Western blotting was used to detect the expression of anti-p21-Ras scFv in tumors from nude mice. Briefly, total proteins were extracted from fresh tumor tissues in the CIK + KGHV500 group and the KGHV500 group. The total proteins were electrophoresed on SDS-PAGE gels, transferred to polyvinylidene fluoride (PVDF) membranes at 4°C for 2 hr, and then blocked with 5% fat-free milk. The membrane was incubated with the anti-FLAG tag mouse mAb or the mouse anti-β-actin mAb (ZSGB-Bio, TA-09, China), followed by incubation with goat anti-mouse IgG and horseradish peroxidase (HRP) (ZSGB-Bio, ZB-5305, China) and DAB solution. Finally, the expression of the anti-p21-RAS scFv was estimated by the depth and width of the color band.
4
Quantification and Visualization of IκB-α and TLR4 Proteins
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Total protein was prepared using RIPA lysis buffer (Beyotime, Haimen, China) and quantified by BCA protein assay kit (Beyotime). Equal amounts of proteins were separated by 10% SDS-PAGE, trans-blotted onto an NC membrane, and incubated with rabbit anti-IκB α antibody (1:2000, Abcam, Cambridge, MA, USA), rabbit anti-IκB α antibody (phospho S36, 1:5000, Abcam), rabbit anti-TLR4 antibody (1:2000, Abcam), and mouse anti-β actin mAb (1:5000, ZSGB BIO, Beijing, China) at 4 °C overnight. After probing with HRP-conjugated secondary antibodies (Dingguo, Beijing, China) at room temperature for 1 h, protein bands were visualized using ECL Plus Reagent (Solarbio) on DNR chemiluminescence detection system. The bands were analyzed using ImageJ software.
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