Nod cd45

NOD/LtDvs (hereafter NOD) mice are maintained at the Medical College of Wisconsin (MCW) by brother-sister mating. ICR/HaJ (ICR), NOD.Cd45.2, and NOD.Cd1d^−/− mice were purchased from The Jackson Laboratory (TJL) (stock numbers 009122, 014149, and 006330 respectively) and maintained by brother-sister mating at the MCW. Five-week-old male NOD/ShiLtJ, CBA/J, BALB/cJ, 129/SvInJ, A/J, FVB/J, SJL/J, C57BL6/J, and DBA2/J were purchased from TJL and housed in the MCW vivarium for two weeks before the experiments. NOD mice were outcrossed to ICR mice to generate reciprocal F1 females that were subsequently mated to NOD males to generate first backcross (BC1) progeny. A total of 163 male BC1 mice were generated for analysis. Results from all BC1 males derived from both directions of the F1 crosses were combined for statistical analyses. All BC1 mice were analyzed at 7 weeks old. Previous studies showed sex difference in the frequency of thymic iNKT-cells^{10 (link)}. Based on this reason, male mice were used in all analyses. All animal protocols were approved by the MCW Institutional Animal Care and Use Committees.

All mouse strains were purchased from the Jackson Laboratory and bred in our barrier facility: NOD (#001976), NOD.SCID (#001303), NOD.Thy1.1 (#004483), NOD.CD45.2 (#14149) and T-cell receptor transgenic (TCR-Tg) mice: BDC2.5 (#004460), BDC12–4.1 (#006303/006304) and NY8.3 (#005868). TCR-Tg T cells from these mice respectively recognize the p79/2.5 mimotope (2.5mi) [23 ], InsB_9–23 epitopes and mimotopes [24 (link)], and IGRP_206–214 epitope, all encoded by our constructs AI and BS. BDC12–4.1.TCRαKO mice were produced as previously described [18 (link)]. Male and female mice donors and recipients were used at 8–16 weeks of age for studies of T-cell response and female NOD mice were used at 10 weeks of age for preclinical experiment. All studies were approved by Columbia University’s Institutional Animal Care and Use Committee.