CD4-Percp (eBioscience, 46-0041), IL-4-APC (MACS, 130-103-002), IL-5-PE (eBioscience, 12-7052), and IL-13-PE Cy7 (eBioscience, 25-7133) were used for flow cytometry. Anti-IL-33 (Abcam, Ab54385) was used for immunofluorescence of mice lung tissues. Anti-PTRF (CST, 46379), anti-Flag (Sigma-Aldrich, M2), anti-HA (Santa Cruz, sc-7392), anti-GFP (Santa Cruz, sc-9996), anti-pTyr (Santa Cruz, sc-7020), anti-β-Actin (Sungene biotech, KM9001T), anti-α-Tubulin (Sungen biotech, KM9003T), and anti-Lamin B (CST, 13435) were used for immunoblotting.
Il 5 pe
Manufactured by Thermo Fisher Scientific
The IL-5-PE is a laboratory instrument used for the detection and analysis of interleukin-5 (IL-5) in biological samples. It is a flow cytometry-based detection system that utilizes a PE (phycoerythrin) fluorescent label to identify and quantify IL-5-positive cells.
Automatically generated - may contain errors
Lab products found in correlation
Cd4 percp, by Thermo Fisher Scientific (1 mentions)
Anti flag, by Merck Group (1 mentions)
Anti il 33, by Abcam (1 mentions)
Il 13 pe cy7, by Thermo Fisher Scientific (1 mentions)
Anti ha, by Santa Cruz Biotechnology (1 mentions)
Anti gfp, by Santa Cruz Biotechnology (1 mentions)
Anti p tyr, by Santa Cruz Biotechnology (1 mentions)
Anti β actin, by Sungene Biotech (1 mentions)
Anti ifn γ fitc, by BioLegend (1 mentions)
Ionomycin, by Merck Group (1 mentions)
Brefeldin a, by BD (1 mentions)
Brefeldin a, by Thermo Fisher Scientific (1 mentions)
Il 4 apc, by Thermo Fisher Scientific (1 mentions)
Ova peptide, by GenScript (1 mentions)
3 protocols using il 5 pe
1
Immunological and Biochemical Profiling
2
Cytokine Production in T Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cells were incubated with culture media containing 200 μg ml−1 brefeldin A (BD Biosciences) with or without stimulation with 1 × phorbol 12-myristate 13-acetate (Calbiochem, EMD Chemicals, Inc., Gibbstown, NJ, USA) and ionomycin (1 μg ml−1; Sigma, St Louis, MO, USA) for 4 h at 37 °C. The plate was then centrifuged at 1000 r.p.m. for 3 min at 4 °C. After surface staining with anti-human anti-CD3, CD4 and CD8, the cells were fixed and permeabilized for intracellular staining. Cells were incubated with anti-TNF-α-allophycocyanin (Biolegend), anti-IFN-γ-FITC (Biolegend) and IL-5-PE (eBioscience). Briefly, cells were washed in wash buffer and resuspended in 200 μl of wash buffer and transferred into fluorescence-activated cell sorting tubes to be analyzed with flow cytometry as described above.
3
T-cell Cytokine Response Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
DO11.10+/CD4+ T-cells and BMDCs were co-cultured as described in the section “Stimulation of carboxyfluorescein succinimidyl ester (CFSE)-labeled OVA-specific T-cells” with or without 20 μg/mL OVA peptide (sequence 323–339; GenScript) for 72 hours. On day 3, Brefeldin A (20 μg/mL; eBioscience) was added to the cultures for 2 hours. At the end of the culture period, cells were incubated with antibodies against CD4 and the DO11.10 T-cell receptor (KJ-126) for 20 minutes on ice prior to fixation in 1% formalin solution for 20 minutes. Cells were incubated in permeabilization buffer (PBS + 0.1% saponin +10% FCS) for 5 minutes at room temperature before the following anti-cytokine antibodies were added for 30 minutes: tumor necrosis factor (TNF)α-eF450, interferon (IFN)γ-eF450, interleukin (IL)10-PE, IL5-PE, IL17-PE-Cy7, and IL4-APC (all eBioscience).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!