The short-chain volatile fatty acids (acetate, butyrate, propionate, iso-butyrate, valerate, and isovalerate) were measured from colonic content samples using the Agilent 6890 gas chromatography (Agilent Technologies, Inc, Palo Alto, CA) according to the previous study [23 ]. To ensure the uniformity of colonic chyme samples, freeze-dried samples were prepared by Vacuum freeze-drying machine (Hrist ALPHA 2–4/LSC, Germany) at −80 °C. Simply, the freeze-dried sample (about 1 g) was weighed into a 10 mL centrifuge tube, mixed evenly with 8 mL ddH2O, centrifuge 7000 g in a sealed tube at 4 °C for 10 min. The mixture of supernatant and 25% metaphosphoric acid solution (0.9 mL and 0.1 mL, respectively) was centrifuged at 20 000×g and 4 °C for 10 min after standing for more than 2 h in a 2 mL sealed tube at 4 °C. The supernatant was filtered by 0.45 μm polysulfone filter and analyzed by gas chromatography with Agilent 6890 (Agilent Technologies, Inc, Palo Alto, CA, USA) with a flame ionization detector and a 1.82 m × 0.2 mm I.D. glass column that was packed with 10% SP-1200/1% H3PO4 on the 80/100 Chromosorb W AW (HP, Inc., Boise, ID, USA).
Agilent 6890
Manufactured by Agilent Technologies
Sourced in United States, Germany, Switzerland
The Agilent 6890 is a gas chromatograph (GC) system designed for analytical and research applications. It features high performance, reliability, and flexibility. The Agilent 6890 is capable of separating and analyzing complex mixtures of chemicals in a variety of industries, including environmental, food, and pharmaceutical.
Automatically generated - may contain errors
Lab products found in correlation
Agilent 5973, by Agilent Technologies (9 mentions)
Agilent 5975, by Agilent Technologies (6 mentions)
Agilent 5973n, by Agilent Technologies (4 mentions)
Chemstation software, by Agilent Technologies (3 mentions)
Hp 5973 quadrupole mass selective detector, by Hewlett-Packard (3 mentions)
Hp 5ms capillary column, by Hewlett-Packard (3 mentions)
Fused silica capillary column, by Merck Group (2 mentions)
Db 5 column, by Agilent Technologies (2 mentions)
Agilent 50 m 0.2 mm 0 5 μm 95 5 methyl, by Agilent Technologies (2 mentions)
Silica gel, by Merck Group (2 mentions)
Agilent 5973n mass spectrometer, by Agilent Technologies (2 mentions)
Hp 5973, by Agilent Technologies (2 mentions)
Agilent 5975c msd, by Agilent Technologies (2 mentions)
Agilent chemstation software, by Agilent Technologies (2 mentions)
Combi pal, by CTC Analytics (2 mentions)
10 m cp sil 19 capillary column, by Agilent Technologies (2 mentions)
Electronic tissue disruptor, by Qiagen (2 mentions)
Agilent 7890, by Agilent Technologies (2 mentions)
Speedvac, by Thermo Fisher Scientific (2 mentions)
5975c mass selective detector, by Agilent Technologies (2 mentions)
189 protocols using agilent 6890
1
Measuring Volatile Fatty Acids in Colonic Content
2
GC-FID Analysis of Short-Chain Fatty Acids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Gas chromatography (GC) is most used for SCFA analysis due to compatibility with the chemical properties of SCFA, such as volatility. Flame ionization detection (FID) is commonly used to detect the SCFA due to its inexpensive cost for operation, as well as its ability to detect a wide range of organic compounds. Therefore, GC was used for the determination of SCFA profile in this study. The SCFA profile of overnight cultured supernatant was analyzed by using GC (GC, Agilent 6890; Agilent Technologies, Palo Alto, CA, USA). The composition of acetic, propionic, iso-butyric, butyric, iso-valeric, valeric and caproic acids were determined for SCFA of cultured supernatant. To determine the SCFA concentration, 0.5 mL of 4-methyl-n-valeric acid (Sigma, Missouri, USA) was added to 0.5 mL of each cultured supernatant as an internal standard before being analysed by GC. The SCFA analysis was performed using a fused-silica capillary column (30 m × 0.25 µm × 0.25 µm) (Agilent 6890; Agilent Technologies, Palo Alto, California, USA) in a GC that equipped with FID detector. The flow rate of nitrogen as the carrier gas was 1.0 mL/ min. The running conditions for SCFA analyses were maintained at 160 °C with FID detector at 250 °C and injector temperature at 230 °C. The GC analyses for SCFA were conducted in triplicate for each metabolite.
3
Fatty Acid Profiling of Omega-3 PUFA Concentrates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fatty acid composition of SFBO and prepared ω-3 PUFAs concentrates were determined using a 6890 Agilent (Agilent Technologies, Wilmington, USA) gas chromatograph facilitated with a fused silica capillary column (100 m length × 0.25 mm internal diameter, 0.2 μm of film) (Supelco, Bellefonte, USA). Fatty acid methyl esters for SFBO, 2-MAG, PUFA enriched acylglycerols, and ω-3 PUFFAs concentrate were prepared according to the methods of American Oil Chemists’ Society (AOCS), Ce 2-66 (2) and Ce 2-66 (3) [34 ], respectively. Oven temperature was programmed to start with a constant temperature of 130 °C for 3 min, then increased to 240 °C at a rate of 4 °C/min, and then held at 240 °C for 10 min. The injector and detector temperature were maintained at 250 °C. Standard fatty acid methyl esters were used for identifying the fatty acid methyl esters and quantification was done by obtained peak area (%).
4
Fatty Acid Composition Analysis by GC-FID
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The fatty acid composition of the samples was analysed by the following steps. In the first step, oil samples for fatty acid analysis (GC-FID) were prepared according to our previous work [4 (link)]. A small amount (0.05 g) of fresh oil was mixed with 0.1% heptadecanoic acid (C17H34O2) mixed with HPLC-grade hexane (2 mL). Afterward, solution was mixed with 3 mL 0.5 N NaOH solution. The mixed solution was mixed thoroughly for about 30 s and heated for nearly 45 min at 75 °C in a sealed bottle. The boiled sample was cooled for 10 min at room temperature and again mixed with 3 mL boron trifluoride (BF3) solution and again subjected to heat at the same temperature (75 °C) for 20 min. After heating, the solution was cooled at room temperature and mixed with 3 m L HPLC-grade hexane and afterward 1 mL 10% NaCl solution. The solution was separated into two parts and the transparent solution from the top parts of the solution was taken using a 3 mL syringe and filtered by a 0.20 µm hydrophobic filter (PTFE-D, Model SD13P020NL, Hyundai micro, Seoul, Korea). The fatty acid composition was analysed by gas chromatography using a 6890 Agilent (Agilent Technologies, Wilmington, NC, USA) gas chromatography following Table S2 .
5
Measuring Ethylene Evolution in Peach Fruit
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ethylene evolution in 'Flatbeauti' peach was measured during fruit development in experiments 2 and 3. Whole-flower and -fruit ethylene production of each experimental plot was determined by enclosing flowers and fruitlets in jars (0.1 to 0.5 L) sealed with a rubber cap and kept in the light at 25 °C. After 2 h, a 1-ml air sample was withdrawn from each jar for ethylene measurement. A gas chromatography 6890 Agilent (Agilent Technologies, Wilmington, Germany) equipped with a flame ionization detector and an alumina column was used for quantifying ethylene concentrations.
6
GC-MS Analysis of Fly Cuticular Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fly body extracts were obtained by washing single flies of the respective sex and mating status in 10 μl of hexane for 30 min. For GC stimulation, 1 μl of the odor sample was injected in a DB5 column (Agilent Technologies; www.agilent.com), fitted in an Agilent 6890 gas chromatograph, and operated as described previously 89 . The inlet temperature was set to 250°C. The temperature of the GC oven was held at 50°C for 2 min, increased gradually (15°C/min) to 250°C, which was held for 3 min, and then to 280°C (20°C/min) and held for 30 min. The MS transfer-line, source and quad were held at 280°C, 230°C and 150°C, respectively.
7
Quantification of Short-Chain Fatty Acids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For measurement of SCFAs in BCS, 200 μl of BCS was acidified by adding 0.1 ml of 50% (v/v) sulfuric acid. After vortexing and standing for 2 min, the organic acids were extracted by adding 0.4 ml of diethyl ether. Then the concentration of SCFAs was measured using the Agilent 6890 (Agilent Technologies, CA, USA) with flame ionization, thermal conductivity detectors, and capillary column. For the determination of SCFAs in cecum content, 200 mg cecum content was homogenized with 1 ml PBS and centrifuged at 16000 × g for 15 min (4°C). The supernatants were filtered through 0.22 μm filters. Determination of SCFAs in BCS was as described above.
8
Measuring Ethylene Release in Treated Leaves
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ethylene (ET) concentration was measured from the head space of vials containing a detached leaf previously treated in planta with ACF or PDB. Samples were taken at 12, 24 and 48 hpt and maintained in sealed vials for 24 h. 1 cm 3 of gas was taken and injected in an Agilent 6890 gas chromatographic system (Agilent Technologies, Santa Clara, California, USA) equipped with a 30 m 9 0.53 mm alumina column. Injector, column, FID temperature, and carrier gas (N 2 ) flow rate conditions were 120, 80, 240 °C, and 0.50 ml s -1 , respectively. ET 123 Biological control of strawberry grey mold disease caused by... quantification was carried out using a calibration curve made with standard ET of known concentration. Results were expressed as nmol ET g -1 leaf fresh weight (FW). Ten plants were evaluated by treatment (n = 10), and the assay was repeated three independent times.
9
Fabrication and Characterization of SOFC Button Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The detailed fabrication procedure, particularly the sequence of
infiltration and heat treatment, of the SOFC button cell can be found
in theSupporting Information . Briefly,
the configuration of the cell comprised a dense YSZ electrolyte layer
(∼70 μm-thick) with two porous YSZ scaffolds (∼45
μm-thick) at the opposite sides. LST and La0.6Sr0.4Fe0O3 (LSF) were the anode and cathode
backbone materials, respectively. Stoichiometric amounts of cobalt
nitrate and ammonium paratungstate were infiltrated to the anode,
calcined, and reduced in H2 at 900 °C for 4 h to obtain
Co7W6@W. Besides, ∼0.5 wt % Pt was infiltrated
into both electrodes to enhance the electrocatalytic performance,
particularly the oxygen activation, below 650 °C.
The gaseous
products were periodically sampled and analyzed with
a gas chromatograph (GC, Agilent 6890) equipped with both FID and
TCD detectors. Conversion of n-butane and selectivity
for the dehydrogenation products including butenes and 1,3-butadiene
were calculated on the basis of carbon balance. The yield of all products
was calculated by multiplying conversion of n-butane
and selectivity of the specific product. The electrochemical analyses
of the cells were performed using both the Gamry Reference 600 potentiostat
and the Solartron SI 1287 electrochemical interface equipped with
an SI 1260 impedance/gain-phase analyzer.
infiltration and heat treatment, of the SOFC button cell can be found
in the
the configuration of the cell comprised a dense YSZ electrolyte layer
(∼70 μm-thick) with two porous YSZ scaffolds (∼45
μm-thick) at the opposite sides. LST and La0.6Sr0.4Fe0O3 (LSF) were the anode and cathode
backbone materials, respectively. Stoichiometric amounts of cobalt
nitrate and ammonium paratungstate were infiltrated to the anode,
calcined, and reduced in H2 at 900 °C for 4 h to obtain
Co7W6@W. Besides, ∼0.5 wt % Pt was infiltrated
into both electrodes to enhance the electrocatalytic performance,
particularly the oxygen activation, below 650 °C.
The gaseous
products were periodically sampled and analyzed with
a gas chromatograph (GC, Agilent 6890) equipped with both FID and
TCD detectors. Conversion of n-butane and selectivity
for the dehydrogenation products including butenes and 1,3-butadiene
were calculated on the basis of carbon balance. The yield of all products
was calculated by multiplying conversion of n-butane
and selectivity of the specific product. The electrochemical analyses
of the cells were performed using both the Gamry Reference 600 potentiostat
and the Solartron SI 1287 electrochemical interface equipped with
an SI 1260 impedance/gain-phase analyzer.
10
Cecal Chyme Fatty Acid Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Two grams of cecal chyme were placed in a centrifuge tube followed by the addition of 5 ml of ultra-pure water, vortex shock for 5 min, and 5,000× g centrifugation for 10 min. Next, 1 ml of the supernatant was placed in a plastic ampere tube; 0.2 ml of 25% phosphoric acid solution was added; and the resulting solution was placed in an ice bath for 30 min followed by 10,000× g centrifugation for 10 min. Then, the supernatant was analyzed on a Hewlett Packard Agilent 6,890 gas chromatograph (Agilent, Palo Alto, CA, USA) to determine the concentrations of cecal chyme acetic acid, propionic acid and butyric acid.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!