Mtt and tunel assay kits

U251 human glioma and U-87 MG human glioblastoma cell lines were purchased from the Chinese Type Culture Collection (CTCC, Shanghai, China) and were maintained in Dulbecco’s modified Eagle’s medium low Glucose (DMEM, SH30021.01, Thermo Scientific HyClone, Beijing, China) supplemented with 50 U/mL of a penicillin/streptomycin mixture (Solarbio Biotech, Beijing, China) and 10% fetal bovine serum (Sijiqing Biotech, Hangzhou, China). CD133⁺ cells were maintained in DMEM-F12 medium supplemented with 20 ng/ml of recombinant human epidermal growth factor (EGF) (10605-HNAE) and recombinant human basic fibroblast growth factor (bFGF) (10014-HNAE) and 2% B27 supplement (Gibco, Grand Island, NY, USA) and 1% penicillin/streptomycin solution (Solarbio Biotech). The cells were routinely grown in 60-cm² cell culture plates (Corning Incorporated, Corning, NY, USA) at 37 °C in a humidified atmosphere with 5% carbon dioxide (CO₂). Honokiol and Stattic were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). MTT and TUNEL assay kits were purchased from Beyotime Biotechnology (Haimen, Jiangsu, China).

Normal human astroglia HEB cell line, SHG44 and U251 human glioma, and U-87 MG human glioblastoma cell lines were purchased from the Chinese Type Culture Collection (CTCC, Shanghai, China) and were maintained in Dulbecco’s modified Eagle’s medium low Glucose (DMEM, Thermo Scientific HyClone, Beijing, China) supplemented with 50 U/mL of a penicillin/streptomycin mixture (Solarbio Biotech Corp., Beijing, China) and 10% fetal bovine serum (Sijiqing Biotech Corp., Hangzhou, China). The cells were routinely grown in 60-cm² cell culture plates (Corning Inc., Corning, NY, United States) at 37°C in a humidified atmosphere with 5% carbon dioxide. Trimebutine (#K1313, sc-204928) was obtained from Santa Cruz Biotechnology, Dallas, TX, United States. MTT and TUNEL assay kits were purchased from Beyotime Biotechnology, Jiangsu, China.