Alpha glucosidase enzyme from saccharomyces cerevisiae

Alpha-glucosidase enzyme from Saccharomyces cerevisiae, α-amylase from porcine pancreas and 2-chloro-4-nitrophenyl-α-D-maltotrioside (CNPG3) were purchased from Sigma Aldrich. All other chemicals were purchased from UFC Biotechnology. A microplate reader (Anthos Zenyth-200RT, Cambridge, UK) was used for the spectrophotometric measurements. A Bruker NMR Spectrometer (Bruker, Yokohama, Japan) used for ¹H-NMR (400 MH_Z) and ¹³C-NMR (100 MHz) analysis. Spectra were recorded in CD₃OD or DMSO-d₆ using tetramethylsilane (Sigma-Aldrich, St. Louis, MO, USA) as an internal standard, and chemical shift values were expressed in δ ppm.

The chemicals used for the experiments were all fresh and of analytical grade. Yeast extract (YE, Oxoid), magnesium sulfate (MgSO₄, Bendosen), dipotassium phosphate (K₂HPO₄, Bendosen), mono-potassium phosphate (KH₂PO₄, Bendosen), ammonium chloride (NH₄Cl, Bendosen), and D (+)- Glucose (R&M Chemicals). Alpha-glucosidase enzyme from Saccharomyces cerevisiae (Sigma-Aldrich), 4-Nitrophenyl glucopyranoside (pNPG, Sigma-Aldrich), heparin sodium salt (Solarbio), and Streptozotocin (Santa Cruz) were purchased in lyophilized powder form.

Analytical grade chemicals such as acetic acid, chloroform, ethanol, methanol, sulfuric acid, powdered glucose, mercuric iodide, nitric acid, potassium sodium tartrate, anhydrous sodium carbonate, sodium hydroxide, iodine, potassium dichromate, bismuth nitrate and ammonia solution were all obtained from Merck, Germany. Tartaric acid, picric acid, lead acetate, potassium dihydrogen phosphate and ferric chloride were supplied by Loba, India. Other reagents include distilled water, castor oil, potassium iodide (Sigma-Aldrich, Germany), DPPH (Sigma-Aldrich, USA), alpha-naphthol (Unichem, China), cupric sulfate (Qualikems, India), sodium bicarbonate (Guangdong Guanghua chemical factory, China), polysorbate-80 (Research-Lab Fine Chem Industries, India), alpha-glucosidase enzyme from Saccharomyces cerevisiae, para-nitrophenyl-α-D-glucopyranoside (pNPG) (Sigma-Aldrich, Switzerland). Standard drugs diclofenac sodium, glibenclamide and voglibose used in this study were procured from Beximco Pharmaceuticals Ltd., Bangladesh.