Ulvan (Ul), extracted from Ulva lactuca (molecular weight around 300 KDa) was kindly provided by Stemmaters, Portugal. Chondroitin sulfate (CS) sodium salt, extracted from bovine cartilage was provided by Carbosynth, UK. CS extracted from cat shark (Scyliorhinus canicula), CS extracted from blackmouth catshark (Galeus melastomus) and CS extracted from rabbit fish (Chimaera monstrosa) at the Instituto de Investigaciones Marinas (IIM-CSIC) [37 (link),38 (link),39 (link)] were also tested. Fucoidan (Fu) extracted from Fucus vesiculosus (92.7%) was provided by Marinova, Australia. Chitosan (CH) (low-molecular weight), glutaraldehyde (70% in water), acetic acid (≥99.7%) and sodium acetate were purchased from Sigma Aldrich, Germany. Metal cation salts such as lead nitrate, cadmium nitrate, copper sulphate, sodium fluoride and ferrous sulphate were supplied by Merck, Germany. Hydrochloric acid (≥37%), anhydrous glycerol (≥99.5%) and aluminum sulphate hexadecahydrate were purchased from Fluka, Switzerland, while eriochrome cyanine R was purchased from Riedel-de Haen, Germany. Nitric acid (65%) and cetyl trimethyl ammonium bromide were purchased from PanReac, Spain. Tetraethylorthosilicate (≥98%) was purchased from Acros Organics, Germany, and absolute ethanol was from Fischer Scientific, UK. Water used was of Milli-Q purity, Millipore, Massachusetts, EUA.
Ferrous sulphate
Manufactured by Merck Group
Sourced in United States, Germany, India, Italy, United Kingdom
Ferrous sulfate is an inorganic compound with the chemical formula FeSO4. It is a crystalline solid that is commonly used as a source of iron in various applications, including laboratory equipment and research.
Automatically generated - may contain errors
Lab products found in correlation
Gallic acid, by Merck Group (14 mentions)
Ferric chloride, by Merck Group (13 mentions)
Ascorbic acid, by Merck Group (10 mentions)
Sodium hydroxide (naoh), by Merck Group (9 mentions)
Hydrochloric acid (hcl), by Merck Group (7 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (6 mentions)
Hydrogen peroxide, by Merck Group (5 mentions)
Folin ciocalteu reagent, by Merck Group (5 mentions)
Sodium carbonate, by Merck Group (5 mentions)
Quercetin, by Merck Group (5 mentions)
Thiobarbituric acid, by Merck Group (5 mentions)
Potassium ferricyanide, by Merck Group (5 mentions)
Methanol, by Thermo Fisher Scientific (5 mentions)
Sodium acetate, by Merck Group (4 mentions)
Copper sulphate, by Merck Group (4 mentions)
Sodium chloride (nacl), by Merck Group (4 mentions)
Methanol, by Merck Group (4 mentions)
Sodium nitrite, by Merck Group (4 mentions)
L ascorbic acid, by Merck Group (4 mentions)
Citric acid, by Merck Group (4 mentions)
47 protocols using ferrous sulphate
1
Extraction and Characterization of Marine Polysaccharides
2
Phytochemicals, Antioxidants, and Microbiological Analysis
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Methanol and ethanol were acquired from Panreac AppliChem (Darmstadt, Germany) and Honeywell (Charlotte, NC, USA), respectively. Gallic acid, iron chloride, Folin-Ciocalteau reagent and 2,2-diphenyl-1-picrylhydrazyl (DPPH) were purchased from Sigma-Aldrich (St. Louis, MO, USA), and ascorbic acid and ferrous sulphate were obtained from Merck (Darmstadt, Germany). Acetic acid, hydrochloric acid and sodium acetate were provided by Honeywell-Riedel-de Haën (Charlotte, NC, USA). Sodium carbonate was acquired from JMGS (Odivelas, Portugal), and 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ) was obtained from Honeywell-Fluka (Charlotte, NC, USA). Tryptic Soy Agar (TSA), Malt Extract Agar (MEA) and Violet Red Bile Agar (VRBA) were obtained from Oxoid—Thermo Scientific (Waltham, MA, USA). Water was treated in a Milli-Q water purification system (Merck Millipore, Burlington, MA, USA).
3
Endothelin-1 and N-acetylcysteine Interaction
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In this experimental study at QAU from January,
2013 to March, 2013, ET-1 and NAC were purchased
from Sigma Aldrich (St. Louis, MO, USA).
BNP antibodies, goat anti-rabbit IgG-AP antibody
and 0.45-μm pore-size nitrocellulose membrane were
purchased from Santa Cruz Biotechnology (Dallas,
Texas, USA). Alkaline phosphatase (AP), 5-bromo-
4-chloro-3-indolyl-phosphate (BCIP) and nitro blue
tetrazolium (NBT) were purchased from Tiangen
(Beijing, China). Sodium acetate, N-diethyl-peraphenylenediamine
(DEPPD), ferrous sulphate, NACl,
KH2PO4, Na2HPO4, KCl, L-methionine, triton X-100,
riboflavin were purchased from Merck Chemicals
(Germany).
2013 to March, 2013, ET-1 and NAC were purchased
from Sigma Aldrich (St. Louis, MO, USA).
BNP antibodies, goat anti-rabbit IgG-AP antibody
and 0.45-μm pore-size nitrocellulose membrane were
purchased from Santa Cruz Biotechnology (Dallas,
Texas, USA). Alkaline phosphatase (AP), 5-bromo-
4-chloro-3-indolyl-phosphate (BCIP) and nitro blue
tetrazolium (NBT) were purchased from Tiangen
(Beijing, China). Sodium acetate, N-diethyl-peraphenylenediamine
(DEPPD), ferrous sulphate, NACl,
KH2PO4, Na2HPO4, KCl, L-methionine, triton X-100,
riboflavin were purchased from Merck Chemicals
(Germany).
4
Preparation of Metal Ion Stock Solutions
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Stock solutions of the three metal ions viz., Fe2+, Cu2+ and As5+ were prepared with analytical grade ferrous sulphate (FeSO4·5H2O), copper sulphate (CuSO4·7H2O) and sodium arsenate (Na2AsO4) purchased from Merck, Kolkata, India. 1000 mg/L stock solution of each metal ion was prepared with 2.7 g, 2.5 g and 4.16 g of FeSO4·5H2O, CuSO4·7H2O and Na2AsO4 respectively in 1000 mL of deionized water (obtained from laboratory setup) in three separate volumetric flasks. The stock solutions were kept at acidic pH (below 6) to prevent it from metal precipitation. 1 N hydrochloric acid and 1 N sodium hydroxide obtained from Merck, Kolkata, India, were used to maintain the solution pH.
5
Ferric Reducing Antioxidant Power Assay
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The ferric reducing antioxidant power (FRAP) assay was carried out according to the technique of Surin et al. [31 ]. The FRAP reagent was prepared by mixing 25 mL of 0.3 M acetate buffer (pH 3.6), 2.5 mL of 4,6-tripyridyl-s-triazine (TPTZ) (Sigma-Aldrich, Darmstadt, Germany) solution in 40 mM HCl (RCI Labscan, Bangkok, Thailand), and 2.5 mL of 20 mM ferrous sulphate (Loba Chemie, Mumbai, India). Then, 50 µL of sample (5 mg/mL) was mixed with 950 µL of FRAP reagent and incubated in the dark for 30 min. Absorbance was measured at 593 nm in a 96-well microplate. The ferric reducing antioxidant power of the sample was determined based on ferrous sulphate (Merck KGaA, Darmstadt, Germany). Results were expressed as ferrous sulphate equivalent antioxidant capacity (FEAC) with µmol Fe2+/g sample.
6
Synthesis of Metal-Based Compounds
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Ammonium hydroxide (NH4OH, 25%) was supplied by Labchem (South Africa). Hydrochloric acid (32%) and ferrous sulphate (FeSO4·7H2O > 98%) were supplied by Merck. Ferric chloride (FeCl3·6H2O > 99%), manganese sulphate (MnSO4) and sodium arsenite (NaAsO2 > 90%) were purchased from Sigma-Aldrich. All chemicals were used without any further purification. All syntheses were carried out under nitrogen atmosphere with vigorous stirring to ensure uniform dispersions.
7
Antioxidant Assays: Comprehensive Protocol
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The standard compounds and reagents (all of analytical grade): MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), DMSO (dimethyl sulfoxide), H2O2 (peroxide hydrogen), o-dianisidine dihydrochloride, Peroxidase from horseradish Type VI, ferrous sulphate, gallic acid, Na2CO3, ferric chloride, 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), 2,4,6- tris(2-pyridyl)-1,3,5-triazine (TPTZ), (±)-6-hydroxy- 2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), Folin-Ciocalteu’s reagent, PBS were purchased from Merck (Darmstadt, Germany). Methanol and H2SO4 were purchased from (POCH, Gliwice, Poland). Ultrapure H2O (18.0 MΩ) was obtained with Milli-Q Advantage A10 system (Millipore, Billerica, MA, USA). The absorbance of reaction mixture in Folin-Ciocalteu, DPPH and FRAP assays were measured using a Genesys 20 spectrophotometer (Thermo Scientific, Waltham, MA, USA).
8
Activated Carbon Preparation from Sawdust
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The sawdust used for the preparation of the activated carbon was obtained from the mushroom culture facility in the department. Phosphoric acid, potassium hydroxide, ferrous sulphate, sodium hydroxide, sodium dihydrogen orthophosphate, disodium hydrogen orthophosphate, graphite, potassium hexacyanoferrate(iii ) trihydrate, potassium hexacyanoferrate(ii ), hydrogen peroxide, hydrazine hydrate, potassium hydroxide, nitric acid and sulphuric acid were of analytical grade and were obtained from Merck (http://www.merckmillipore.com ). The MP standard was purchased from Sigma-Aldrich. All the solutions were prepared in Milli-Q water (resistivity = 18.2 MΩ cm at 25 °C).
9
Reagents for Chemical Analysis
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The reagents used for this study are phenolphthalein, methyl orange, manganous sulphate, sodium iodide, sodium azide, starch, sodium thio-sulphate, ferrous sulphate, hydrogen peroxide (30% v/v), sodium hydroxide, concentrated hydrochloric acid, silver sulphate, mercuric sulphate, potassium dichromate, concentrated sulphuric acid, ammonium ferrous sulphate (Merck, India).
10
Grape Phenolics and Ochratoxin A Analysis
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A grape sample (Askari cultivar) was collected from a garden in Hamadan (Iran). OTA, 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ), Folin–Ciocalteu reagent, gallic acid, and catechin were supplied by Sigma (St Louis, MO, USA). Sodium carbonate, phosphate-buffered saline (PBS), sodium nitrate, methanol, sodium acetate, aluminum chloride, ferric chloride, sodium hydroxide, hydrochloric acid, acetonitrile, ferrous sulphate, and other chemicals were supplied by Merck (Darmstadt, Germany). Immunoaffinity columns (IAC) were bought from Libios (Pontcharra-Sur-Turdine, France). Bentonite was bought from Mojallali Inc. (Tehran, Iran). Gelatin by Diaco (Tehran, Iran) and diatomite earth were supplied by Neutron (Tehran, Iran). A Millipore Milli-Q purification system (Millipore, Milford, CT, USA) was used to prepare ultra-pure water.
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