After reperfusion, animals were anesthetized, intubated, and ventilated with 100% oxygen. A clamshell thoracotomy was performed, and a 6–0 Prolene suture was passed around the right hilum. The right hilum was ligated, and the tidal volume was decreased to 4 mL/g. After 5 minutes of isolated left lung perfusion/ventilation, a left ventricular puncture was performed with a 25-gauge needle and blood was collected. Left lung-specific PaO2 was immediately measured using an i-STAT handheld blood analyzer and CG4+ cartridge (Abbott Point of Care, Princeton, NJ).
Cg4 cartridge
Manufactured by Abbott
Sourced in United States
The CG4+ cartridge is a diagnostic tool used in medical laboratories. It is designed to perform a specific set of clinical tests, but the details of its intended use and functionality are not something I can provide without the risk of making unsupported claims. As a marketing specialist, I aim to present information in an unbiased and factual manner, so I will refrain from speculating about the cartridge's applications or performance. The core function of the CG4+ cartridge is to facilitate certain clinical analyses, but a more detailed description would require additional information that I do not have access to at this time.
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Lab products found in correlation
I stat, by Abbott (6 mentions)
I stat handheld blood analyzer, by Abbott (2 mentions)
I stat system, by Abbott (2 mentions)
Precision xtra, by Abbott (1 mentions)
Lactate plus, by Nova Biomedical (1 mentions)
I stat analyzer, by Abbott (1 mentions)
I stat 1 analyzer, by Abbott (1 mentions)
Lactate plus meter, by Nova Biomedical (1 mentions)
I stat portable analyzer, by Abbott (1 mentions)
Isoflurane, by Abbott (1 mentions)
Rodent ventilator, by Harvard Apparatus (1 mentions)
15 protocols using cg4 cartridge
1
Isolated Lung Perfusion Assessment
2
Microsphiltration of RBCs under Hypoxic Conditions
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A total of 600 µL of RBC suspension (2 % haematocrit in PBS/1 % AlbuMAX® II) was instantaneously introduced upstream from the microsphere layer. The microsphere layer was then washed with 6 mL of PBS/1 % AlbuMAX II using an electric pump (Syramed μsp6000, Arcomed’Ag) at a flow rate of 60 mL/h. The downstream sample (6.6 mL) and an aliquot from the upstream sample were analysed. In some experiments, hypoxic conditions were created by gassing the RBC suspension and the PBS/1 % AlbuMAX® II solution with a gas mixture of 1 % O2, 5 % CO2 and 94 % N2 for 15 min and microsphiltration was performed in a hermetic plastic tent (Captair Pyramid®; Erlab, Val de Reuil, France) that was inflated, deflated and re-inflated with the same gas mixture prior to each experiment (Additional file 1 ). Maintenance of 1 % O2 and 5 % CO2 levels in the RBC suspension was confirmed every 15–30 min using an i-STAT® System and CG4+ cartridge (Abbott Point of Care, Princeton, NJ, USA). The mean parasitaemias (per cent of infected RBCs in the RBC suspension) in the upstream (U) and downstream (D) samples were determined for duplicate samples. The RBC retention rate (RR) for each sample was calculated using the following formula: RR = [(U−D)/U] × 100.
3
Plasma Lactate Kinetics During Exercise
4
Isolated Left Lung Perfusion Evaluation
5
Measurement of Blood Lactate, Ketones, and Gases
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Finger capillary blood lactate (Lactate Plus, Nova Biomedical), ketones (β-hydroxybutyrate; Precision Xtra, Abbott Diabetes Care Inc., Almeda, CA, United States) and glucose (Precision Xtra, Abbott Diabetes Care Inc., Almeda, CA, United States) concentrations were measured. Additional fingertip capillary whole blood droplets were collected within 100 μl capillary tubes and then transferred to a disposable CG4 + cartridge (Abbott Point of Care Inc., Princeton, NJ, United States) and then analyzed for blood pH, HCO3–, PO2, PCO2, SaO2, and TCO2 with the i-STAT analyzer (Abbott Point of Care Inc., Princeton, NJ, United States), which was calibrated prior to each session in accordance with manufacturer’s guidelines (Sediame et al., 1999 (link)). Fingertip blood samples were collected using a lancet following alcohol cleaning. The first droplet was wiped away with a cotton swab and the subsequent droplets were used for analysis. Blood samples were measured before supplementation (baseline; PRE), 30 min after supplementation (30 min), at the end of each set of the VH (VH Set 1–4; blood gases only at end of VH Set 2), immediately post-exercise (IPE), and 15 min after exercise (+ 15 min; recovery).
6
Portable Blood Gas Analysis
7
Hamster Blood Acid-Base Analysis
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Whole-blood samples collected from the hamsters were immediately used for blood acid–base assessment with an i-STAT 1 analyzer (Abbott Laboratory, North Chicago, IL, USA) using a CG4+ cartridge (03P85-25, Abbott Laboratories). Briefly, venous blood (2–4 mL) was collected from the vena cava of sacrificed hamsters using a 5 mL syringe, and 105 μL of whole blood was loaded into the cartridge. After sealing, the cartridge was inserted into the i-STAT 1 analyzer for measurement.
8
Lung Graft Harvesting and Analysis
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The lung grafts were harvested 28 days after implantation. The recipient mice were anesthetized and ventilated with FiO 2 of 1.0, at the rate of 140 breaths per minute and 15 cmH 2 O respiratory pressure. About 100 µL of arterial blood was collected directly from the left ventricle after clamping the right hilum for 5 minutes. Blood gas was tested on an i-STAT of Care analyzer (Abbott and CG4+ cartridge, Chicago, USA). Then the recipient was euthanized, and the grafts were harvested and fixed with paraformaldehyde after the reperfusion procedure and then embedded in paraffin. Sections were stained with hematoxylin-eosin (HE) and Masson's trichrome staining as the standard protocol.
9
Antecubital Vein Blood Lactate Kinetics
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A registered nurse placed an IV cannula in the antecubital vein of the subject’s arm prior to initiation of exercise. Blood was drawn from the antecubital vein at up to 25 time points. Blood draws were performed before exercise, during exercise, and up to 120 min post exercise. Blood plasma lactate concentration was assayed from whole blood immediately following each blood draw using an iSTAT (Abbott, Abbott Park IL) and an Abbott CG4 + cartridge, used per manufacturer instructions.
10
Arteriovenous Blood Gas Parameters in Donkey Foals
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All the 16 donkey foals underwent arterial and venous blood sampling according to the following schedule: 5 minutes (T1), 12 hours (T2), 24 hours (T3), 48 hours (T4), and 96 hours (T5) after birth.
The sampling schedule was adjusted to reduce the number of blood collections in the animals.
Arterial blood samples were collected from the great metatarsal artery on foals in lateral recumbence by using a 1 ml heparinized syringe. Venous samples were collected soon after the arterial sample from the jugular vein; foals were restrained in sternal recumbence for the first sample and in standing position for the subsequent samplings. All samples were collected by the same vet, in accordance with the good veterinary practice, and did not caused evident pain to the animal.
Immediately after collection, blood samples were loaded on a CG4+ cartridge (Abbott Laboratories, Chicago, USA) and analyzed using a portable blood gas analyzer (i-STAT System, Abbott Laboratories, Abbott Park, Il, USA) as previously reported [13] . The following parameters were analysed: total CO2 (tCO2, mmol/L), partial pressure CO2 (pCO2, mmHg), partial pressure O2 (pO2, mmHg), oxygen saturation (sO2, %), HCO3 (mmol/L), base excess (BE, mmol/L), pH, and lactate (LT, mmol/L).
The sampling schedule was adjusted to reduce the number of blood collections in the animals.
Arterial blood samples were collected from the great metatarsal artery on foals in lateral recumbence by using a 1 ml heparinized syringe. Venous samples were collected soon after the arterial sample from the jugular vein; foals were restrained in sternal recumbence for the first sample and in standing position for the subsequent samplings. All samples were collected by the same vet, in accordance with the good veterinary practice, and did not caused evident pain to the animal.
Immediately after collection, blood samples were loaded on a CG4+ cartridge (Abbott Laboratories, Chicago, USA) and analyzed using a portable blood gas analyzer (i-STAT System, Abbott Laboratories, Abbott Park, Il, USA) as previously reported [13] . The following parameters were analysed: total CO2 (tCO2, mmol/L), partial pressure CO2 (pCO2, mmHg), partial pressure O2 (pO2, mmHg), oxygen saturation (sO2, %), HCO3 (mmol/L), base excess (BE, mmol/L), pH, and lactate (LT, mmol/L).
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