HCC cells were irradiated by different radioactive rays Gy (0.5, 1.0, 1.5, 2.0, 2.5 and 3.0) from 6Mv-X-ray produced by a linear accelerator (Varian 600, Varian Medical Systems). The following day after irradiation, cells were used as MTT assy. Cells treated with 2 Gy radioactive rays were used as colony formation assay and TUNEL assay.
Varian 600
Manufactured by Agilent Technologies
Sourced in United States
The Varian 600 is a high-performance nuclear magnetic resonance (NMR) spectrometer designed for analytical and research applications. It is capable of operating at a frequency of 600 MHz, providing high-resolution data for the analysis of chemical structures and molecular compositions.
Automatically generated - may contain errors
4 protocols using varian 600
1
Radiotherapy Effects on HCC Cells
2
Modulating circRNA and miRNA in Glioblastoma
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Irradiation was performed in a linear accelerator (Varian 600, Varian, USA) at a dose rate of 3.2 Gy/min. circ_0012381 overexpression, circ_0012381 inhibitor, circRNA control, miR-340-5p overexpression, miR-340-5p inhibitor, and miRNA control lentiviruses were synthesized by Genechem (Shanghai, China) (Additional file 1 : Table S1). To block exosome generation, U251 or U87 cells were incubated with 10 µM GW4869 (Sigma-Aldrich), which was initially dissolved in DMSO into a stock solution of 5 mM and diluted in culture medium, for 24 h.
3
Dose-dependent X-ray Irradiation Effects
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4
Spectroscopic Characterization of Compounds
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1D and 2D NMR spectra were recorded on Bruker DRX 400 (Bruker BioSpin GmbH, Rheinstetten, Germany) and Varian 300 and Varian 600 (Varian, Inc., Palo Alto, CA, USA) spectrometers, using standard Bruker or Varian pulse sequences. Chemical shifts internally referenced to residual solvent signals are given on a δ (ppm) scale. High-resolution ESI and APCI mass spectra were measured on a Thermo Scientific LTQ Orbitrap Velos mass spectrometer (ThermoFisher Scientific, Bremen, Germany). Column chromatography separations were performed with Kieselgel Si 60 (Merck, Darmstadt, Germany). HPLC separations were conducted on a CECIL 1100 Series liquid chromatography pump (Cecil Instruments Ltd., Cambridge, UK) equipped with a GBC LC-1240 refractive index detector (GBC Scientific Equipment, Braeside, VIC, Australia), using a 250 mm × 22 mm i.d. Techsil 10 ODS column (Wellington House, Cheshire, UK) for reversed-phase HPLC or a 250 mm × 10 mm i.d. Kromasil 100-10-SIL (Akzonobel, Eka Chemicals AB, Separation Products, Bohus, Sweden) for normal-phase HPLC. TLC was performed on Kieselgel 60 F254 (0.2 mm) precoated aluminum or glass plates (Merck, Darmstadt, Germany), and spots were visualized after spraying with H2SO4 in MeOH (20% v/v) reagent and heating at 100 °C for 1 min.
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