The concentration of IL-1β was quantified by Human IL-1β kit (RD, cat# DLB50), mouse IL-1β kit (RD, cat# MLB00C), and rat IL-1β kit (RD, cat# RLB00), respectively. All samples were added in triplicate, the protein concentration was calculated from the standard curve. The serum and cultured cells LDH was determined by Lactate Dehydrogenase (LDH) Assay kit (Solarbio, cat# BC0685). All operations are performed in strict accordance with the operating instructions.
Lactate dehydrogenase ldh assay kit
Manufactured by Solarbio
Sourced in Switzerland
The Lactate Dehydrogenase (LDH) Assay kit is a colorimetric assay used to quantify the activity of the LDH enzyme. LDH catalyzes the conversion of lactate to pyruvate, with the concurrent reduction of NAD+ to NADH. The assay kit measures this NADH production, providing a direct measurement of LDH activity in the sample.
Automatically generated - may contain errors
Lab products found in correlation
Parkin, by Santa Cruz Biotechnology (1 mentions)
α tubulin, by Beyotime (1 mentions)
Mitochondrial membrane potential assay kit with jc 1 c2006, by Beyotime (1 mentions)
Pyruvate kinase assay kit, by Solarbio (1 mentions)
Lcmt 1, by Santa Cruz Biotechnology (1 mentions)
Anti lc3b antibody, by Abcam (1 mentions)
Pme 1, by Santa Cruz Biotechnology (1 mentions)
Atp assay kit, by Beyotime (1 mentions)
Mito tracker green, by Beyotime (1 mentions)
Faststart universal sybr green master mix rox, by Roche (1 mentions)
Lyso tracker red, by Beyotime (1 mentions)
2 protocols using lactate dehydrogenase ldh assay kit
1
Quantification of Cytokine and LDH Levels
2
Methionine Starvation and Mitophagy Modulation
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Phorbol 12-myristate 13-acetate (PMA, P8139, purity > 99%), taurine powder (T0625, purity > 99%), ABL127 (SML0294, purity > 98%), Lipopolysaccharides (LPS, L2630), Methionine-free medium (RPMI-1640 medium, R7513), and methionine powder (L-Methionine, M9625, purity > 98%) were purchased from Sigma-Aldrich Inc. (St. Louis, MO, USA). Cell culture medium (RPMI-1640 medium, 3180022) was purchased from Gibco-Life Technologies (Grand Island, USA). FastStart Universal SYBR Green Master Mix (ROX) (4913914001) was obtained from Roche (Basel, Switzerland); Hexokinase (HK) Assay Kit (BC0680), pyruvate kinase (PK) Assay Kit (BC0545), Lactate dehydrogenase (LDH) Assay Kit (BC0685), Pyruvic acid dehydrogenase (PDH) Assay Kit (BC0385) and Neutral Red solution, 0.33% (G1310) were purchased from Solarbio (Shanghai, China); ATP assay kit (S0026), Mito-Tracker Green (C1048), Mitochondrial membrane potential assay kit with JC-1 (C2006), Lyso-Tracker Red (C1046) and α-Tubulin were obtained from Beyotime (Jiangsu, China). Beclin-1, Cryopyrin (NLRP3), PINK1, Parkin, demethylated PP2Ac, methylated PP2Ac, LCMT-1, PME-1, total PP2Ac antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-LC3B antibodies were purchased from Abcam Co. (Cambridge, UK). SQSTM1 (p62) and Bafilomycin A1 (BAF) (54645) were purchased from Cell Signaling Technology Co. (Danvers, MA).
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