To evaluate the fluorescein leakage and retinal morphology after intravitreal injection of Hcy into wild-type and NMDAR−/−R mice, OCT and FA were performed according to our published methods with some modifications [9 (link),11 (link),16 (link),17 (link),37 (link)]. Briefly, the mice were injected intravitreally with Hcy (200 μM) and, after 72 h, OCT and FA were conducted. The mice were subjected to anesthesia using 2% isoflurane and the eye pupils were dilated by 1% tropicamide eye drop. Then, each mouse was placed and imaged on the imaging platform of the Phoenix Micron III retinal imaging microscope supplemented with an OCT imaging device (Phoenix Research Laboratories, Pleasanton, CA, USA). Lubricant gel was added to the eye to keep it moist during imaging. For FA, 10% fluorescein sodium (Apollo Ophthalmic, Newport Beach, CA, USA) was injected into the mice (10 to 20 μL, IP) followed by rapid acquisition of fluorescent images succeeded for ~5 min. Fluorescein leakage establishes as indistinct vascular borders gradually progressing to diffusely hazy green fluorescence.
Oct imaging device
Manufactured by Phoenix Pharmaceuticals
Sourced in United States
The OCT imaging device is a non-invasive, high-resolution imaging tool that uses light to capture cross-sectional images of biological structures, such as the retina and other tissues. It provides detailed information about the morphology and structural changes within the imaged area.
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4 protocols using oct imaging device
1
Retinal Imaging to Evaluate Hcy-Induced Effects
2
In Vivo Retinal Morphology Evaluation
3
Mouse Fundus and OCT Imaging
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Fundus imaging and SD-OCT were done as previously described [58 (link),59 (link)]. Briefly, 2% isoflurane was used to anesthetize the mice, and their pupils were dilated using 1% tropicamide eye drops. Mice were placed on the imaging platform of the Phoenix Micron III retinal imaging microscope, supplemented with an OCT imaging device (Phoenix Research Laboratories, Pleasanton, CA). Genteal gel was applied during imaging to keep the eye moist. Four images were taken for each mouse eye.
4
Multimodal Imaging of Retinal RPE
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To evaluate retinal RPE structure and function in vivo, OCT and FA were performed simultaneously as described previously with some modifications [41 (link)]. Briefly, mice were anesthetized using 2% isoflurane and their pupils were dilated using 1% tropicamide eye drop. Each mouse was then placed on the imaging platform of the Phoenix Micron III retinal imaging microscope supplemented with OCT imaging device (Phoenix Research Laboratories, Pleasanton, CA). Genteal gel was applied liberally to keep the eye moist during imaging. Mice were administered 10 to 20 μL 10% fluorescein sodium (Apollo Ophthalmics, Newport Beach, CA), and rapid acquisition of fluorescent images ensued for ∼5 minutes. Fluorescein leakage manifests as indistinct vascular borders progressing to diffusely hazy fluorescence.
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