Dulbecco s modified eagle s medium dmem and fetal bovine serum

Dehydrocostus Lactone (C₁₅H₁₈O₂, MW,230.3, purity > 98%) was purchased from Target Molecule Corp. (Target Mol, Shanghai, China). LPS (Escherichia coli, serotype 055:B5), collagenase and DNase were purchased from Sigma Chemical Co. (St Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) was purchased from Gibco (Thermo Fisher Scientific, Waltham, MA, USA). The bicinchoninic acid (BCA) protein assay kit, NO assay kit (Griess reagent), and phenylmethylsulfonyl fluoride (PMSF) were purchased from Beyotime Biotechnology (Shanghai, China). Primary antibodies against p-Akt(S473), Akt, p-p65 (S536), p65, p-p38, p38, p-MK2, MK2 and β-actin were purchased from Cell Signaling Technology (Danvers, MA, USA).

We obtained 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) from A.V.T. Pharmaceutical Co., Ltd. (Shanghai, China), and phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-SS-PEG-2000) from Xi'an ruixi Biological Technology Co., Ltd. (Xi'an, China). Meanwhile, we acquired 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide (DiR), LysoTracker Green, and 3-(4,5)-dimethylthiahiazol(-z-y1)-3,5-di-phenytetrazoliumbromide (MTT) from Sigma-Aldrich. We purchased Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) from Gibco (USA) and Sal from Selleck Chemicals (USA). Moreover, we obtained FITC-EpCAM and PE-CD133 antibodies from BioLegend (Shanghai, China).

Luteolin and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) from Sigma (USA); Dulbecco’s Modified Eagle’s Medium (DMEM) and fetal bovine serum (FBS) from Gibco BRL (USA); methanol and acetic acid (HPLC grade) from Fisher Scientific (UK); and dimethyl sulfoxide (DMSO) and acetone from KeLong Chemicals (China). Antibodies purchased include: rat anti-mouse CD31 polyclonal antibody (BD PharmingenTM, USA), rabbit anti-mouse Ki67 antibody (Abcam, USA), and rhodamine-conjugated secondary antibody (Abcam, USA).
The MPEG (molecular weight = 2000) (Sigma–Aldrich, St. Louis, MO, USA) was dried in a one-necked flask under vacuum and magnetically stirred at 105 °C for 90 min before use.
MPEG (2000)-PCL(2000) diblock copolymer with a molecular weight of 4000 was synthesized by ring opening of ε-caprolactone, which was initiated by MPEG as descripting previously [21 (link)]. Briefly, MPEG and ε-CL were introduced into a dry glass ampoule under a nitrogen atmosphere. Sn(Oct)2 was then added into the reaction vessel under mild agitation, and the reaction system was kept at 130 °C for 6 h. The purified MPEG-PCL copolymer was kept in a desiccator before further use.