The treatment modalities of MDA-MB-231 cells was done to mimic the clinical NAC treatment of breast cancer patients. Briefly, MDA-MB-231 cells were treated in vitro with four cycles combination of 200 nM doxorubicin (Brand name Adriamycin, Pharmacia, Italy) and 600 nM cyclophosphamide (4xAC) (Brand name Cytoxan, Baxter, Germany) followed by four cycles of 50 nM paclitaxel (4xAC+4xPAC) (Brand name Taxol, EBEWE Pharma, Austria). Each treatment cycle was 72 h long. After each cycle, the cells which remained attached were left to proliferate until confluency and the following cycle of treatment was initiated right after.
Cytoxan
Manufactured by Baxter
Sourced in Germany
Cytoxan is a pharmaceutical product used in medical laboratories. It is a sterile powder for injection, intended for intravenous administration. The active ingredient in Cytoxan is cyclophosphamide, a chemotherapeutic agent.
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using cytoxan
1
Neoadjuvant Chemotherapy Simulation in MDA-MB-231
2
Phage Therapy in Murine ExPEC Infection
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This model system is described in detail in reference36 (link). Briefly, mice were gavaged with 109 CFU of ExPEC strain JJ2528 and animals administered cyclophosphamide (Cytoxan, Baxter Healthcare Corporation, Deerfield, IL, USA) IP for a total dose 450 mg/kg body weight on days 1, 3 and 5 as indicated in Fig. 3 . Purified ϕHP3 was administered IP on the days 2, 4 and 6 as indicated in Fig. 3 and the mice necropsied on day 7 post infection.
3
Neutropenic Lung Infection Model for Acinetobacter baumannii
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Neutropenic lung infection model for A. baumannii was used for mouse infection studies47 (link). 6–8 weeks old Female BALB/c mice (Charles River/Hollister) were rendered neutropenic by 2 intraperitoneal injections of Cytoxan (Baxter Health Care Corporation) at 150 mg/kg on Day-4 and 100 mg/kg on Day-1. On day 0, mice were infected intranasally with 50 ul log-phase grown A. baumannii at 107 CFU/mouse. At 2- and 24-h post infection, bacterial load (CFUs) was determined in the lung, spleen, liver and kidneys through serial dilutions.
4
Generating Chemoresistant Breast Cancer Cell Lines
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Chemoresistant BT-474 and BT-474 NRP-1 variant cell lines were generated in a similar protocol to that described previously [13 (link)]. Briefly, the cells were treated with four cycles of a combination of 0.5 uM of Doxorubicin (Brand name Adriamycin, Pharmacia, Italy) + 300 nM Cyclophosphamide (Brand name Cytoxan, Baxter, Germany) (cells will be referred to as 4xAC) followed by four cycles of 20 nM Paclitaxel (Brand name Taxol, EBEWE Pharma, Austria) (cells will be referred to as 4xAC + 4xPAC). Each cycle was for a duration of 72 h followed by a recovery period until confluency was achieved prior to commencement of the next cycle. Protein lysate and RNA was extracted from the 4xAC and 4xAC + 4xPAC resistant cell lines and stored at − 80 °C.
5
Imiquimod-Induced Lupus Nephritis Model
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Age-matched female FVB mice were purchased from Jackson Laboratory and randomized into experimental groups upon receipt. Ten-week-old female FVB mice were treated with 10 mg of 5% Imiquimod cream (Taro Pharmaceuticals, USA, Hawthorne, N.Y.) applied to the left ear (three doses per week for the duration of the study). Mice were then treated with either NIK SMI1 (200 mg/kg, P.O., BID), Cytoxan (Cyclophosphamide, Baxter Healthcare Corporation) (30 mg/kg, I.P. every 10 days), or vehicle control (MCT). Proteinuria and survival were tracked through day 91. Plasma PK measurement was done at day 85 prior to the end of the study.
6
Cyclophosphamide and Sulforaphane in Animal Model
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Cyclophosphamide: Cyclophosphamide (Cytoxan) or Endoxan® 500 mg vial, (Eczacıbaşı Baxter, Turkey) is a nitrogen mustard derivative was supplied as vials from Baxter oncology (Düsseldorf, Germany) (Sigma, St, Louis, Mo, USA).
Sulforaphane: Sulforaphane (Glucosinolate) was purchased as orange-yellow powder from Fluka chemical Co. (Steinheim, Germany) dissolved or diluted in 0.9% sterile saline.
Animals were sacrificed by deep anesthesia using isoflurane inhalation. Urinary bladder was dissected out for the different investigations which described below.
Sulforaphane: Sulforaphane (Glucosinolate) was purchased as orange-yellow powder from Fluka chemical Co. (Steinheim, Germany) dissolved or diluted in 0.9% sterile saline.
Animals were sacrificed by deep anesthesia using isoflurane inhalation. Urinary bladder was dissected out for the different investigations which described below.
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