Fluorochrome antibodies specific for cd4

Splenocytes from DBA/1 mice were isolated (3 (link)) and the level of expression of LAIR1 was determined following overnight culture with each analog at a concentration of 10⁻⁷ M (or ethanol as vehicle control) by multi-parameter flow cytometry using an LSRII flow cytometer (BD Biosciences, San Jose, CA, USA). Cells were labeled with fluorochrome antibodies specific for CD4 (BD Biosciences) and for LAIR1 (eBioscience, San Diego, CA, USA). Gating was performed on CD4+ cells and the data were displayed as mean fluorescence±standard deviation. A minimum of 10,000 cells were analyzed from each treated sample and the final analysis was performed using Flow software (Tree Star, Ashland, OR, USA). Results are expressed as the mean of duplicate values±SEM.

Splenocytes from DBA/1 mice were isolated,^{33 (link)} and the expression of LAIR-1 was determined following overnight culture with each secosteroid at 10⁻⁷ M (or vehicle control) by multiparameter flow cytometry using an LSRII flow cytometer (BD Biosciences, San Jose, CA). Cells were labeled with fluorochrome antibodies specific for CD4 (BD Biosciences, San Jose, CA) and for LAIR-1 (eBioscience, San Diego, CA). Gating was performed on CD4 cells, and the data were expressed as mean fluorescence ± SD. A minimum of 10 000 cells were analyzed from each sample, and the final analysis was performed using Flow software (Tree Star, Ashland, OR). Values represent the mean fluorescence from two separate analyses.