2 phenylethyl isothiocyanate

Reagents were of analytical purity. Media components (peptone, glucose monohydrate, sodium nitrate, potassium hydrogenphosphate, magnesium sulfate, potassium chloride, ferrous sulfate) were from Reanal (Budapest, Hungary). Allyl isothiocyanate, allyl cyanide, 2-phenylethyl isothiocyanate and phenylpropionitrile and glutathione (reduced form) were from Sigma Aldrich (MO, USA). Pure glucosinolates were from Phytoplan (Germany). LC-MS grade acetonitrile, water and formic acid were purchased from Fisher Scientific (Belgium). Solvents (1-propanol, 2-propanol) and media additives (streptomycin, chloramphenicol and dichloran) were from VWR. Double distilled water was used throughout the study.

Benzonitrile (≥99.9%), 3-butenenitrile (2Prop-CN; ≥98%), 3-(methylthio)propyl ITC (3MTP-ITC; ≥98%), 2-propenyl ITC (2Prop-ITC; ≥99%), 4-pentenenitrile (3But-CN; ≥97%), 3-phenylpropanenitrile (2PE-CN; ≥99%), and 2-phenylethyl isothiocyanate (2PE-ITC; ≥99%) were purchased from Sigma–Aldrich Chemie GmbH (Steinheim, Germany). 1-Cyano-2,3-epithiopropane [CETP; ≥97.6% (by GC-MS)] was purchased from Taros Chemicals GmbH Co. KG (Dortmund, Germany). 3-Indoleacetonitrile (IAN) (≥98%) was acquired from Acros Organics (Fischer Scientific GmbH, Schwerte, Germany). 3-Butenyl ITC (3But-ITC; ≥95%) was obtained from TCI Deutschland GmbH (Eschborn, Germany). 3-(Methylsulfinyl)propyl ITC (3MSOP-ITC) and 4-(methylthio)butyl ITC (4MTB-ITC; ≥98%) were purchased from Santa Cruz Biotechnology (Heidelberg, Germany). 4-(Methylsulfinyl)butyl ITC (4MSOB-ITC) was bought from Enzo Life Sciences GmbH (Lörrach, Germany). (R)-5-Vinyloxazolidine-2-thione (R-OZT) was purchased from Biosynth AG (Staad, Switzerland). 4-Hydroxybenzyl GLS (≥99%) and methylene chloride (GC Ultra grade) was obtained from Carl Roth GmbH (Karlsruhe, Germany). Acetonitrile (Ultra Gradient HPLC grade) was procured from J.T. Baker (Deventer, The Netherlands). NaSO₄ (≥99%) and methanol (>99.9) was purchased from VWR International GmbH (Darmstadt, Germany).
All solvents were of LC-MS or GC-MS grade, water was of Milli-Q quality.

15-LOX (lipoxygenase), α-glucosidase, DPPH (2,2-diphenyl-1-picrylhydrazyl radical), bovine serum albumin (BSA), trichloroacetic acid (TCA), methylglyoxal (MGO) and D-glucose were purchased from Merck, (Dorset, UK), and Oxoid (Hampshire, UK), whereas NaN₃ was purchased from DaeJung (Siheung-si, Korea). Chemical for Gel analysis including Coomassie blue, Tris–HCl, sodium dodecyl sulphate, 2-mercaptoethanol, Glycerine, bromophenol blue were purchased from Sigma Aldrich, St. Louis, MO, USA. The standard compounds included aminoguanidine (≥98.5%, Sigma Aldrich, St. Louis, MO USA), eugenol (≥99%, Fluka, Riedstr, Germany), 2-phenylethylisothiocyanate (>99%, Sigma Aldrich, St. Louis, MO, USA), juglone (≥98.5%, Santa Cruz Biotechnology, Santa Cruz, CA, USA), quercetin (≥99%, Sigma Aldrich, St. Louis, MO, USA), quercitrin (≥85%, Sigma Aldrich, St. Louis, MO, USA), trans-caryophyllene (≥98.5%, Fluka, , Riedstr, Germany), α-humulene (>98%, Extrasynthese, Genay, France), caryophyllene-oxide (≥99%, Fluka Honeywell, Seelze, Germany) and apigenin (≥95.5% Sigma Aldrich, St. Louis, MO, USA) (structures are shown in the Supplementary Materials).