Imdm medium

CD19+ Daudi [31 (link)] (EBV positive Burkitt’s lymphoma) and CD19- K562 (chronic myeloid leukemia (CML) cell line and NK target) was purchased from ATCC (cat no CCL-213 and CCL-243, respectively) and cultured in RPMI medium (cat no 21875–034) supplemented with 10% fetal bovine serum (cat no 10500–064) and 1% Penicillin-Streptavidin (cat no 15140–122). 293T (ATCC, cat no CRL-3216) was cultured in IMDM medium (cat no 12440–053) supplemented with 10% fetal bovine serum, 1% Penicillin-Streptavidin and 0.1% Sodium Pyruvate (cat no 11360–070). All cell culture components were purchased from Life Technologies.

Human cell lines promyelocytic leukemia HL-60, acute lymphocytic leukemia lines Reh and RS4:11, acute myeloid leukemia MV-4-11, myelogenous leukemia lines K562 and KG-1a, epidermoid carcinoma A431, and Chinese hamster ovary (CHO) cell line were purchased from American Tissue Culture Collection (ATCC, Manassas, VA). The acute myeloid leukemia MOLM-14 line was purchased from the German Collection of Microorganisms and Cell Lines (DSMZ, Braunschweig Germany). The cell lines with the exception of A431, MV-4-11, and KG-1a, were cultured in RPMI-1640 Medium (ATCC) supplemented with 10% heat-inactivated fetal bovine serum (FBS). The A431 line was cultured in DMEM Medium (ATCC) supplemented with 10% heat inactivated FBS. The MV-4-11 cell line was cultured in IMDM Medium (ATCC) supplemented with 10% heat-inactivated FBS. The KG-1a line was cultured in IMDM Medium supplemented with 20% FBS. Where applicable, luciferase-expressing subclones were generated by stably transducing wild-type leukemia lines with lentiviral vector encoding firefly luciferase with or without GFP (Lentigen Technology, Inc., Gaithersburg, MD), followed by limiting dilution and selection of luciferase-positive clones.

Human umbilical vein endothelial cells (HUVECs; ATCC^® CRL-1730^TM (Manassas, VA, USA)) were cultured in EndoGRO^TM-VEGF complete medium (Merck Millipore, Burlington, MA, USA) supplemented with 1% penicillin-streptomycin (Invitrogen; Waltham, MA, USA). Acute myeloblastic leukemia cell line (KG-1a cells; ATCC^® CCL-246.1^TM) were cultured in IMDM medium (ATCC, Manassas, VA, USA) supplemented with 20% fetal bovine serum (FBS) and 1% penicillin-streptomycin (Invitrogen; Waltham, MA, USA). Both cells were grown at 37 °C in a humidified 5% CO₂ atmosphere. Poly (lactic-co-glycolic acid) (average MW: 24,000–38,000; lactic to glycolic ratio: 50:50), dichloromethane (DCM), and polyvinyl alcohol (PVA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Most materials and reagents were purchased from Sigma-Aldrich unless specified. The antibodies used in this study were purchased from Abcam (Cambridge, UK) unless specified.