Log In Sign up
The largest database of trusted experimental protocols

Six well ultra low cluster plates

Manufactured by Corning
Visit Supplier
Sourced in United States

Six-well ultra-low cluster plates are a type of laboratory equipment designed for cell culture applications. These plates feature a unique surface treatment that minimizes cell attachment and aggregation, promoting the formation of spherical cell clusters or organoids. The six-well format provides a convenient platform for various experiments and assays requiring the cultivation of cells in a low-attachment environment.

Automatically generated - may contain errors

Lab products found in correlation

Epidermal growth factor (egf), by Thermo Fisher Scientific (4 mentions) Dmem f12 serum free medium, by Thermo Fisher Scientific (3 mentions) Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (3 mentions) Bovine serum albumin (bsa), by Merck Group (2 mentions) Epidermal growth factor (egf), by Beyotime (1 mentions) Basic fibroblast growth factor (bfgf), by Beyotime (1 mentions) Bovine serum albumin (bsa), by Sangon (1 mentions) Insulin, by Beyotime (1 mentions) B27 supplement, by Thermo Fisher Scientific (1 mentions) Basic fgf, by Thermo Fisher Scientific (1 mentions) Dmem f12 medium, by Thermo Fisher Scientific (1 mentions) 96 well plate, by Corning (1 mentions) Murine recombinant epidermal growth factor, by Thermo Fisher Scientific (1 mentions) Insulin, by Merck Group (1 mentions)

Spelling variants of this product

Six-well plates (488 citations) Ultra Low Cluster plates (34 citations) Ultra Low plates (3 citations)

7 protocols using six well ultra low cluster plates

1

Mammosphere Culture of MCF7 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dissociated single MCF7-Notch and MCF7-mCMV cells were plated on six-well ultra-low cluster plates (Corning Inc.) at a density of 1×105 cells/ml and grown for seven–ten days. Subsequent cultures after dissociation of primary spheres were plated on new plates at a density of 1×104 cells/ml. Mammosphere cultures were grown in a serum-free mammary epithelium basal medium as previously described (13 (link))
D’Angelo R.C., Ouzounova M., Davis A., Choi D., Tchuenkam S.M., Kim G., Luther T., Quraishi A.A., Senbabaoglu Y., Conley S.J., Clouthier S.G., Hassan K.A., Wicha M.S, & Korkaya H. (2015). Notch reporter activity in breast cancer cell lines identifies a subset of cells with stem cell activity. Molecular cancer therapeutics, 14(3), 779-787.
+ Open protocol
+ Expand
2

Eca109 Spheroid Formation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Eca109 cells were seeded at 1.5 × 103 cells/well in six-well ultra-low cluster plates (Corning) and cultured in DMEM/F12 serum-free medium (Gibco) supplemented with 2% B27 (Invitrogen), 20 ng/mL EGF (PeproTech), and 20 ng/mL bFGF (PeproTech) for 10 days. Subsequently, tumorspheres with diameter larger than 75 μm were counted.
Wang L., Yu X., Zhang Z., Pang L., Xu J., Jiang J., Liang W., Chai Y., Hou J, & Li F. (2017). Linc-ROR promotes esophageal squamous cell carcinoma progression through the derepression of SOX9. Journal of Experimental & Clinical Cancer Research : CR, 36, 182.
+ Open protocol
+ Expand
3

Seeding and Culturing Spheres

Check if the same lab product or an alternative is used in the 5 most similar protocols
Five hundred cells were seeded in six-well ultralow cluster plates (Corning, NY, USA) for 10 days. Spheres were cultured in Dulbecco’s modified Eagle’s medium/F12 serum-free medium (Invitrogen, Grand Island, NY, USA) supplemented with 2% B27 (Invitrogen, Grand Island, NY, USA), 20 ng/ml of EGF, and 20 ng/ml of bFGF (PeproTech, Offenbach, Germany), 0.4% bovine serum albumin (Sigma) and 5 μg/ml insulin.
Chen S., Huang J., Liu Z., Liang Q., Zhang N, & Jin Y. (2017). FAM83A is amplified and promotes cancer stem cell-like traits and chemoresistance in pancreatic cancer. Oncogenesis, 6(3), e300-.
+ Open protocol
+ Expand
4

Sphere Formation Assay for Cancer Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
PC3 and DU145 cells were seeded into six-well ultra-low cluster plates (Corning, NY, USA) according to the number of 1000 cells per well, respectively. Then, these cells were cultured with DMEM/F12 serum-free medium (Invitrogen) supplemented with 20 ng/mL bFGF (Beyotime, Nantong, China), 2% B27 (Thermo Fisher, Waltham, MA, USA), 20 ng/mL EGF (Beyotime, Nantong, China), 5 μg/mL insulin (Beyotime, Nantong, China) and 0.4% BSA (Sangon, Shanghai, China). A count and photograph of spheres were taken after two weeks. (Cell clusters larger than 50 mm in diameter were considered to be CSC)
Liu J., Niraj M., Wang H., Zhang W., Wang R., Kadier A., Li W, & Yao X. (2022). Down-Regulation of lncRNA MBNL1-AS1 Promotes Tumor Stem Cell-like Characteristics and Prostate Cancer Progression through miR-221-3p/CDKN1B/C-myc Axis. Cancers, 14(23), 5783.
+ Open protocol
+ Expand
5

Sphere Formation Assay for Cancer Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Similar to previous studies [7 (link)], a sphere formation assay was performed. Isolated CD133−CD44− and CD133+CD44+ cells (1 × 104 cells/well) were plated on to six-well ultralow cluster plates (Corning, U.S.A.). Cells were cultured in serum-free DMEM-F12 medium (Gibco, U.S.A.) with 20 ng/ml EGF (Peprotech, U.S.A.), B27 supplement (1:50, Gibco, U.S.A.), and 20 ng/ml basic FGF (Peprotech, U.S.A.). The number of cell spheres was counted after 3 weeks of culture.
Peng L., Xiong Y., Wang R., Xiang L., Zhou H, & Gu H. (2020). Identification of a subpopulation of long-term tumor-initiating cells in colon cancer. Bioscience Reports, 40(8), BSR20200437.
+ Open protocol
+ Expand
6

Evaluating RIF1 Knockdown and Overexpression on Cell Viability and Sphere Formation

Check if the same lab product or an alternative is used in the 5 most similar protocols
In total, 5 × 103 RIF1 knockdown or overexpressed cells were cultured in 96-well plates (Corning). Cell Titer 96 Aqueous-One-Solution Cell Proliferation Assay kit (MTS) was used to detect the cell viability.
For sphere formation assay, 1 × 104 cells were cultured in six-well ultra-low cluster plates (Corning) for 7 days. Spheres were cultured in Dulbecco’s modified Eagle’s medium/F12 serum-free medium (Hyclone) added with 40 ng/μl Epidermal growth factor (EGF) (Gibco), B27 (Gibco), 10 ng/μl basic fibroblast growth factor (bFGF) (Peprotech).
Mei Y., Liu Y.B., Cao S., Tian Z.W, & Zhou H.H. (2018). RIF1 promotes tumor growth and cancer stem cell-like traits in NSCLC by protein phosphatase 1-mediated activation of Wnt/β-catenin signaling. Cell Death & Disease, 9(10), 942.
+ Open protocol
+ Expand
7

Serum-Free Culture of LLCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
LLCs were seeded at 300 cells per well in six-well ultralow cluster plates (Corning, 07-200-601) and cultured for 7 days in DMEM/F12 serum-free medium (Gibco, 11320033) supplemented with 20 ng/ml recombinant murine epidermal growth factor (EGF) (PeproTech, 315-09), 10 ng/ml recombinant murine basic fibroblast growth factor (bFGF) (PeproTech, 450-33), 5 μg/ml insulin (Sigma), and 0.4% bovine serum albumin (Sigma).
Zhang L., Du J., Song Q., Zhang C, & Wu X. (2022). A Novel In Situ Dendritic Cell Vaccine Triggered by Rose Bengal Enhances Adaptive Antitumour Immunity. Journal of Immunology Research, 2022, 1178874.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!