Ab81872

Manufactured by Abcam

Ab81872 is a laboratory equipment product. It is used for a specific scientific purpose. The core function of this product is to perform a particular task in a laboratory setting.

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Lab products found in correlation

Facscalibur, by BD (1 mentions) Anti human cd3, by BD (1 mentions) Anti human cd107a, by BD (1 mentions) Anti human cd137, by BD (1 mentions) Accuri c6 cytometer, by BD (1 mentions) Anti human cd8, by BD (1 mentions) Phycoerythrin labeled streptavidin, by Thermo Fisher Scientific (1 mentions) Anti mouse f ab 2 antibody, by Jackson ImmunoResearch (1 mentions)

2 protocols using ab81872

Multiparametric Flow Cytometry Characterization

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Antibodies were obtained from the following suppliers: anti-human CD3 (BD Biosciences, 555335), anti-human CD8 (BD Biosciences 555366), anti-human CD107a (BD Biosciences 555801), anti-human CD137 (BD Biosciences 555956). Cell surface expression of ErbB2 was detected by biotylated anti-ErbB2 Affibody (Abcam, ab31890), and EGFR by FITC conjugated anti-EGFR affibody (Abcam, ab81872). EGFR, ErbB2 and CD19 specific CAR expression were detected by biotin-labeled polyclonal anti-human F(ab)2 antibody for (EGFR CAR) or anti-mouse F(ab)2 antibody (for ErbB2 and CD19 CARs)(Jackson Immunoresearch). Samples were then stained with PE-conjugated anti-human IgG Fc Ab (eBioscience, 12-4998-82) or phycoerythrin-labeled streptavidin (eBioscience, 17-4317-82). Flow cytometry acquisition was performed on either a BD FacsCalibur or Accuri C6 Cytometer (BD Biosciences). Analysis was performed using FlowJo software (Treestar).

Liu X., Jiang S., Fang C., Yang S., Olalere D., Pequignot E.C., Cogdill A.P., Li N., Ramones M., Granda B., Zhou L., Loew A., Young R.M., June C.H, & Zhao Y. (2015). Affinity-tuned ErbB2 or EGFR chimeric antigen receptor T cells exhibit an increased therapeutic index against tumors in mice. Cancer research, 75(17), 3596-3607.

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EGFR Affibody Internalization Assay

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Cells were detached using Ca²⁺/Mg²⁺-free PBS, resuspended in PBS and incubated with EGFR affibody conjugated with FITC (Abcam#ab81872; 1:200) for 30 min. FITC intensity was measured in an Accuri BD FACS Flow Cytometer. To evaluate recycling, a monensin^{64 (link)} (10 μM) 1 h pretreatment was performed. Fluorescence intensity was measured using ImageJ Software and was expressed as integrated density (ID): intensity mean value of the image multiplied by the positive area percentage.

Manzano S., Gutierrez-Uzquiza A., Bragado P., Sequera C., Herranz Ó., Rodrigo-Faus M., Jauregui P., Morgner S., Rubio I., Guerrero C, & Porras A. (2021). C3G downregulation induces the acquisition of a mesenchymal phenotype that enhances aggressiveness of glioblastoma cells. Cell Death & Disease, 12(4), 348.

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