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Anti cd45 pe cy5 clone

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The Anti-CD45 PE-Cy5 (clone) is a laboratory reagent used for the detection and enumeration of CD45-positive cells in flow cytometry. It is a fluorescently-labeled monoclonal antibody that binds specifically to the CD45 antigen, which is expressed on the surface of most leukocytes. The PE-Cy5 fluorochrome allows for the identification and quantification of CD45-positive cells in a sample.

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Lab products found in correlation

Blue viability dye, by Thermo Fisher Scientific (2 mentions) Anti cd3 alexa fluor 700 clone, by Agilent Technologies (2 mentions) Anti cd56 pe cy7, by Agilent Technologies (2 mentions) Anti cd64 fitc clone 10, by Agilent Technologies (2 mentions) Anti cd89 pe clone a, by Agilent Technologies (2 mentions) Anti cd15 pacificblue clone, by Agilent Technologies (2 mentions) Anti cd16 bv510 clone, by Agilent Technologies (2 mentions) Anti cd32 apc clone fun 2, by Agilent Technologies (2 mentions) Anti cd64 r pe clone 10, by Agilent Technologies (2 mentions) Perm a buffer, by Thermo Fisher Scientific (2 mentions) Anti cd68 fitc clone kp1, by Agilent Technologies (2 mentions) Perm b, by Thermo Fisher Scientific (2 mentions) Lsrii flow cytometer, by BD (2 mentions)

2 protocols using anti cd45 pe cy5 clone

1

Comprehensive Fc Receptor Profiling

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Flow cytometric evaluation of Fc receptor expression was performed on freshly isolated cells from enzymatically digested colon and cervical tissues. 1x106 colon or 1x105 cervical cells were stained in two panels with anti-CD3 Alexa Fluor 700 (clone UCHT1), anti-CD56 PE-Cy7 (clone NCAM16.2), anti-CD64 FITC (clone 10.1), anti-CD89 PE (clone A59), anti-CD45 PE-Cy5 (clone HI30), anti-CD15 PacificBlue (clone W6D3), anti-CD16 BV510 (clone 3G8), anti-CD32 APC (clone FUN-2), anti-CD64 R-PE (clone 10.1, Dako), blue viability dye (Life Technologies). Cells were washed and fixed with Perm A buffer (Life Technologies). Intracellular staining with anti-CD68 FITC (clone KP1, Dako) was performed in the presence of permeabilization buffer (Perm B, Life Technologies). The data was acquired using an LSRII flow cytometer (BD Biosciences) and analyzed using FlowJo and SPICE version 5.1.
Sips M., Krykbaeva M., Diefenbach T.J., Ghebremichael M., Bowman B.A., Dugast A.S., Boesch A.W., Streeck H., Kwon D.S., Ackerman M.E., Suscovich T.J., Brouckaert P., Schacker T.W, & Alter G. (2016). Fc Receptor-Mediated Phagocytosis in Tissues as a Potent Mechanism for Preventive and Therapeutic HIV Vaccine Strategies. Mucosal immunology, 9(6), 1584-1595.
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2

Comprehensive Fc Receptor Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Flow cytometric evaluation of Fc receptor expression was performed on freshly isolated cells from enzymatically digested colon and cervical tissues. 1x106 colon or 1x105 cervical cells were stained in two panels with anti-CD3 Alexa Fluor 700 (clone UCHT1), anti-CD56 PE-Cy7 (clone NCAM16.2), anti-CD64 FITC (clone 10.1), anti-CD89 PE (clone A59), anti-CD45 PE-Cy5 (clone HI30), anti-CD15 PacificBlue (clone W6D3), anti-CD16 BV510 (clone 3G8), anti-CD32 APC (clone FUN-2), anti-CD64 R-PE (clone 10.1, Dako), blue viability dye (Life Technologies). Cells were washed and fixed with Perm A buffer (Life Technologies). Intracellular staining with anti-CD68 FITC (clone KP1, Dako) was performed in the presence of permeabilization buffer (Perm B, Life Technologies). The data was acquired using an LSRII flow cytometer (BD Biosciences) and analyzed using FlowJo and SPICE version 5.1.
Sips M., Krykbaeva M., Diefenbach T.J., Ghebremichael M., Bowman B.A., Dugast A.S., Boesch A.W., Streeck H., Kwon D.S., Ackerman M.E., Suscovich T.J., Brouckaert P., Schacker T.W, & Alter G. (2016). Fc Receptor-Mediated Phagocytosis in Tissues as a Potent Mechanism for Preventive and Therapeutic HIV Vaccine Strategies. Mucosal immunology, 9(6), 1584-1595.
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