4 2 hydroxyethyl 1 piperazine ethanesulfonic acid hepes

All the chemicals used in study were either of analytical or molecular biology grade and procured from well-known standard companies. Fetal Bovine Serum (FBS) was purchased from GIBCO-BRL, 4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid (HEPES) from Himedia Laboratories, Mumbai, India, in situ cell death detection kit, POD from Roche Inc., Switzerland, hexane and methanol from SD Fine-Chem Limited, Mumbai, India, copper sulphate, sulphuric acid, hydrochloric acid, sodium chloride, sodium dihydrogen phosphate, disodium hydrogen phosphate, hydrogen peroxide, citric acid, bovine serum albumin (BSA), Folin’s reagent, ammonium chloride, trypan blue, sodium nitrite, phosphoric acid, sulphanilamide, N-(1-napthyl) ethylenediamine dihyrochloride were procured from Merck. All other chemicals were obtained from Sigma-Aldrich unless mentioned otherwise.

M199 medium, Roswell Park Memorial Institute (RPMI) 1640 medium, penicillin G sodium salt, streptomycin sulphate, propidium iodide (PI), glutamine and pentamidine were purchased from Sigma-Aldrich, fetal bovine serum (FBS) from Biowest, dimethylsulfoxide (DMSO) from SRL, 4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid (HEPES) from Himedia, isopropanol and methanol from Merck. Annexin V–FLUOS staining kit and ApoDirect kits were procured from Roche Inc., Switzerland. All the antibodies and their isotype controls were procured from BD Biosciences. All the other chemicals were obtained from Sigma-Aldrich unless stated otherwise.